EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.70 | salicylate | interacts with the isozyme and inhibits its peptidase activity to various degrees both in vitro and in plant extracts; interacts with the isozyme and inhibits its peptidase activity to various degrees both in vitro and in plant extracts. Specific isozyme TOP2salicylate interaction may be dependent on plant-specific modifications of isozyme TOP2 | Arabidopsis thaliana |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.24.70 | chloroplast | - |
Arabidopsis thaliana | 9507 | - |
3.4.24.70 | cytosol | - |
Arabidopsis thaliana | 5829 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.70 | Zn2+ | a zinc-dependent metallopeptidase | Arabidopsis thaliana |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.70 | Arabidopsis thaliana | Q949P2 | isozyme TOP2 | - |
3.4.24.70 | Arabidopsis thaliana | Q94AM1 | isozyme TOP1 | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.24.70 | leaf | - |
Arabidopsis thaliana | - |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.24.70 | Top1 | - |
Arabidopsis thaliana |
3.4.24.70 | Top2 | - |
Arabidopsis thaliana |
EC Number | Organism | Comment | Expression |
---|---|---|---|
3.4.24.70 | Arabidopsis thaliana | expression of isozyme TOP2 is augmented by the flagellin peptide flg22 in an isozyme TOP1-dependent manner | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.4.24.70 | evolution | the TOP isozymes contain the conserved His-Glu-XX-His (HEXXH) sequence of active-site residues and belong to the M3 family of metallopeptidases. TOP1 and TOP2 are located on opposite ends of chromosome 5 and share high homology, indicating the occurrence of a past intra-chromosomal duplication event | Arabidopsis thaliana |
3.4.24.70 | malfunction | plants with defective enzyme expression exhibit heightened resistance to exogenous salicylate treatment | Arabidopsis thaliana |
3.4.24.70 | metabolism | isozymes TOP1 and TOP2 are necessary for effector-triggered plant immunity via RPS2 and RPS4 resistance genes, and regulation of pathogen-triggered programmed cell death. TOP1 and TOP2 expression are coordinately regulated during pathogen-associated molecular pattern-triggered plant immunity | Arabidopsis thaliana |
3.4.24.70 | physiological function | the enzyme is a target for salicylic acid binding and participate in SA-mediated plant innate immunity. Both isozymes TOP1 and TOP2 seem to play a role in salicylic acid-dependent innate immunity, they are necessary for the immune response to avirulent pathogens. Isozyme TOP1 induces the augmentation of expression of isozyme TOP2 by the flagellin peptide flg22. Isozymes TOP1 and TOP2 are necessary for effector-triggered plant immunity via RPS2 and RPS4 resistance genes, and regulation of pathogen-triggered programmed cell death | Arabidopsis thaliana |
3.4.24.70 | physiological function | the enzyme is a target for salicylic acid binding and participate in SA-mediated plant innate immunity. Both isozymes TOP1 and TOP2 seem to play a role in salicylic acid-dependent innate immunity, they are necessary for the immune response to avirulent pathogens. Isozymes TOP1 and TOP2 are necessary for effector-triggered plant immunity via RPS2 and RPS4 resistance genes, and regulation of pathogen-triggered programmed cell death | Arabidopsis thaliana |