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Literature summary extracted from

  • Cuesta-Seijo, J.A.; Nielsen, M.M.; Marri, L.; Tanaka, H.; Beeren, S.R.; Palcic, M.M.
    Structure of starch synthase I from barley: insight into regulatory mechanisms of starch synthase activity (2013), Acta Crystallogr. Sect. D, 69, 1013-1025.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.21 expressed in Escherichia coli Tuner(DE3) cells Hordeum vulgare

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.21 sitting drop vapor diffusion method, using 100 mM sodium cacodylate buffer pH 6.5, 17% (w/v)v PEG 8K, 0.2 M ammonium sulfate Hordeum vulgare

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.21 C126S the mutant shows strongly reduced activity compared to the wild type enzyme Hordeum vulgare
2.4.1.21 C506S the mutant shows strongly reduced activity compared to the wild type enzyme Hordeum vulgare
2.4.1.21 F538A the mutant shows strongly reduced activity compared to the wild type enzyme Hordeum vulgare

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.21 soluble
-
 Hordeum vulgare
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.21 Hordeum vulgare Q9M5A3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.21 nickel-affinity column chromatography and gel filtration Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.21 ADP-alpha-D-glucose + glycogen
-
 Hordeum vulgare ADP + ?
-
 ?
2.4.1.21 ADP-alpha-D-glucose + maltopentaose
-
 Hordeum vulgare ADP + maltohexaose
-
 ?
2.4.1.21 ADP-alpha-D-glucose + maltotriose
-
 Hordeum vulgare ADP + maltotetraose
-
 ?
2.4.1.21 ADP-alpha-D-glucose + soluble starch
-
 Hordeum vulgare ADP + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.21 ADP-glucose:1,4-alpha-D-glucan 4-alpha-D-glucosyltransferase
-
 Hordeum vulgare
2.4.1.21 SSI
-
 Hordeum vulgare
2.4.1.21 starch synthase I
-
 Hordeum vulgare