EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.1.2.1 | purified enzyme in a binary complex with L-serine and in a ternary complex with D-serine and (6R)-5-formyltetrahydrofolate, microbatch method, mixing of 0.001 ml of 0.38 mM protein in 0.86 mM PLP, 62 mM 2-mercaptoethanol, 87 mM Gly, L-Ser, or D-Ser, and 43 mM tetrahydrofolate, with 0.001 ml of well solution containing 20-21% w/v PEG 4000, 70-90 mM NaCl, 100 mM Tris-HCl, pH 8.5, 15% v/v trifluoroethanol, 20°C, 1-4 days, X-ray diffraction structure determination and analysis at 2.4-2.5 A resolution, molecular replacement | Plasmodium vivax |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.1 | additional information | Plasmodium vivax | the enzyme catalyzes the conversion of L- and D-serine to glycine in a THFdependent reaction | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.2.1 | Plasmodium vivax | A5K8L9 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.1 | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
Plasmodium vivax | tetrahydrofolate + L-serine | - |
r | |
2.1.2.1 | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
Plasmodium vivax | tetrahydrofolate + D-serine | - |
r | |
2.1.2.1 | additional information | the enzyme catalyzes the conversion of L- and D-serine to glycine in a THFdependent reaction | Plasmodium vivax | ? | - |
? | |
2.1.2.1 | additional information | L- and D-serine substrate binding structure analysis, overview | Plasmodium vivax | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.2.1 | serine hydroxymethyltransferase | - |
Plasmodium vivax |
2.1.2.1 | SHMT | - |
Plasmodium vivax |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.2.1 | 25 | - |
assay at | Plasmodium vivax |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.2.1 | 8 | - |
assay at | Plasmodium vivax |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.2.1 | 5,10-methylenetetrahydrofolate | - |
Plasmodium vivax | |
2.1.2.1 | pyridoxal 5'-phosphate | - |
Plasmodium vivax | |
2.1.2.1 | tetrahydrofolate | binding structure analysis | Plasmodium vivax |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.1.2.1 | metabolism | the enzyme is involved in folate recycling and dTMP synthesis | Plasmodium vivax |