EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.B34 | expression of N-terminally truncated variant, in Escherichia coli | Limosilactobacillus reuteri |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.B34 | 150 | - |
maltose | relaese of glucose, pH 4.7, 37°C | Limosilactobacillus reuteri |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.B34 | 154000 | - |
x * 154000, calculated | Limosilactobacillus reuteri |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.B34 | Limosilactobacillus reuteri | A5VL73 | - |
- |
2.4.1.B34 | Limosilactobacillus reuteri | Q5SBN1 | - |
- |
2.4.1.B34 | Limosilactobacillus reuteri DSM 20016 | A5VL73 | - |
- |
2.4.1.B34 | Limosilactobacillus reuteri ML1 | Q5SBN1 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.B34 | Maltoheptaose | - |
Limosilactobacillus reuteri | ? | - |
? | |
2.4.1.B34 | Maltoheptaose | - |
Limosilactobacillus reuteri ML1 | ? | - |
? | |
2.4.1.B34 | Maltoheptaose | - |
Limosilactobacillus reuteri DSM 20016 | ? | - |
? | |
2.4.1.B34 | Maltohexaose | - |
Limosilactobacillus reuteri | ? | - |
? | |
2.4.1.B34 | Maltohexaose | - |
Limosilactobacillus reuteri ML1 | ? | - |
? | |
2.4.1.B34 | Maltohexaose | - |
Limosilactobacillus reuteri DSM 20016 | ? | - |
? | |
2.4.1.B34 | maltopentaose | - |
Limosilactobacillus reuteri | ? | - |
? | |
2.4.1.B34 | maltopentaose | - |
Limosilactobacillus reuteri ML1 | ? | - |
? | |
2.4.1.B34 | maltopentaose | - |
Limosilactobacillus reuteri DSM 20016 | ? | - |
? | |
2.4.1.B34 | maltose | - |
Limosilactobacillus reuteri | ? | - |
? | |
2.4.1.B34 | maltotetraose + H2O | - |
Limosilactobacillus reuteri | D-glucose + maltotetraose | first clear products of the reaction. The enzyme is rather hydrolytic at the start of the reaction. When reaction products start to accumulate, transglycosylation becomes more efficient | ? | |
2.4.1.B34 | maltotetraose + H2O | - |
Limosilactobacillus reuteri ML1 | D-glucose + maltotetraose | first clear products of the reaction. The enzyme is rather hydrolytic at the start of the reaction. When reaction products start to accumulate, transglycosylation becomes more efficient | ? | |
2.4.1.B34 | maltotriose | - |
Limosilactobacillus reuteri | ? | - |
? | |
2.4.1.B34 | maltotriose | - |
Limosilactobacillus reuteri DSM 20016 | ? | - |
? | |
2.4.1.B34 | additional information | enzyme cleaves alpha1->4 glycosidic linkages and adds the released glucose moieties one by one to the non-reducing end of growing linear alpha-glucan chains via alpha1->6 glycosidic linkages (alpha1->4 to alpha1->6 transfer activity). It converts pure maltooligosaccharide substrates into linear alpha-glucan product mixtures with about 50% alpha1->6 glycosidic bonds (isomalto/maltooligosaccharides). Largest products synthesized from maltoheptaose have a degree of polymerizatition bleow 50 | Limosilactobacillus reuteri | ? | - |
? | |
2.4.1.B34 | additional information | enzyme cleaves alpha1->4 glycosidic linkages and adds the released glucose moieties one by one to the non-reducing end of growing linear alpha-glucan chains via alpha1->6 glycosidic linkages (alpha1->4 to alpha1->6 transfer activity). It converts pure maltooligosaccharide substrates into linear alpha-glucan product mixtures with about 50% alpha1->6 glycosidic bonds (isomalto/maltooligosaccharides). Largest products synthesized from maltoheptaose have a degree of polymerizatition bleow 50 | Limosilactobacillus reuteri ML1 | ? | - |
? | |
2.4.1.B34 | additional information | enzyme cleaves alpha1->4 glycosidic linkages and adds the released glucose moieties one by one to the non-reducing end of growing linear alpha-glucan chains via alpha1->6 glycosidic linkages (alpha1->4 to alpha1->6 transfer activity). It converts pure maltooligosaccharide substrates into linear alpha-glucan product mixtures with about 50% alpha1->6 glycosidic bonds (isomalto/maltooligosaccharides). Largest products synthesized from maltoheptaose have a degree of polymerizatition bleow 50 | Limosilactobacillus reuteri DSM 20016 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.B34 | ? | x * 154000, calculated | Limosilactobacillus reuteri |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.B34 | GflML4 | - |
Limosilactobacillus reuteri |
2.4.1.B34 | GtfW | - |
Limosilactobacillus reuteri |
2.4.1.B34 | Lreu_1346 | - |
Limosilactobacillus reuteri |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.B34 | 37 | - |
assay at | Limosilactobacillus reuteri |
2.4.1.B34 | 40 | 45 | - |
Limosilactobacillus reuteri |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.B34 | 37 | - |
maltose | relaese of glucose, pH 4.7, 37°C | Limosilactobacillus reuteri |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.B34 | 4.5 | - |
- |
Limosilactobacillus reuteri |
2.4.1.B34 | 4.7 | - |
assay at | Limosilactobacillus reuteri |