EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.99.54 | expressed and produced in a protease/(hemi-) cellulase-free Myceliophthora thermophila C1 strain | Thermothelomyces thermophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.99.54 | 22770 | - |
mass spectrometry | Thermothelomyces thermophilus |
1.14.99.54 | 23000 | - |
SDS-PAGE | Thermothelomyces thermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.99.54 | additional information | Thermothelomyces thermophilus | The enzyme also oxidizes wheat arabinoxylan, birchwood glucuronoxylan and oat spelt xylan if assayed in the presence of amorphous cellulose. The enzyme uses cellulose to bind while oxidizing neighboring xylan chains. No activity is observed with wheat arabinoxylan, birchwood glucuronoxylan and oat spelt xylan alone | ? | - |
? | |
1.14.99.54 | additional information | Thermothelomyces thermophilus C1 | The enzyme also oxidizes wheat arabinoxylan, birchwood glucuronoxylan and oat spelt xylan if assayed in the presence of amorphous cellulose. The enzyme uses cellulose to bind while oxidizing neighboring xylan chains. No activity is observed with wheat arabinoxylan, birchwood glucuronoxylan and oat spelt xylan alone | ? | - |
? | |
1.14.99.54 | [(1->4)-beta-D-glucosyl]n+m + AH2 + O2 | Thermothelomyces thermophilus | - |
[(1->4)-beta-D-glucosyl]m-1-(1->4)-D-glucono-1,5-lactone + [(1->4)-beta-D-glucosyl]n + A + H2O | - |
? | |
1.14.99.54 | [(1->4)-beta-D-glucosyl]n+m + AH2 + O2 | Thermothelomyces thermophilus C1 | - |
[(1->4)-beta-D-glucosyl]m-1-(1->4)-D-glucono-1,5-lactone + [(1->4)-beta-D-glucosyl]n + A + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.99.54 | Thermothelomyces thermophilus | A0A0H4K9X4 | Thielavia heterothallica | - |
1.14.99.54 | Thermothelomyces thermophilus C1 | A0A0H4K9X4 | Thielavia heterothallica | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.14.99.54 | methylation | the enzyme contains an N-methylated histidine at the N-terminus | Thermothelomyces thermophilus |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.99.54 | - |
Thermothelomyces thermophilus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.99.54 | [(1->4)-beta-D-glucosyl]n+m + reduced acceptor + O2 = [(1->4)-beta-D-glucosyl]m-1-(1->4)-D-glucono-1,5-lactone + [(1->4)-beta-D-glucosyl]n + acceptor + H2O | (1) the D-glucono-1,5-lactone at the reducing end of the cellulose chain quickly hydrolyzes spontaneuosly to the aldonic acid | Thermothelomyces thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.99.54 | additional information | The enzyme also oxidizes wheat arabinoxylan, birchwood glucuronoxylan and oat spelt xylan if assayed in the presence of amorphous cellulose. The enzyme uses cellulose to bind while oxidizing neighboring xylan chains. No activity is observed with wheat arabinoxylan, birchwood glucuronoxylan and oat spelt xylan alone | Thermothelomyces thermophilus | ? | - |
? | |
1.14.99.54 | additional information | The enzyme also oxidizes wheat arabinoxylan, birchwood glucuronoxylan and oat spelt xylan if assayed in the presence of amorphous cellulose. The enzyme uses cellulose to bind while oxidizing neighboring xylan chains. No activity is observed with wheat arabinoxylan, birchwood glucuronoxylan and oat spelt xylan alone | Thermothelomyces thermophilus C1 | ? | - |
? | |
1.14.99.54 | [(1->4)-beta-D-glucosyl]n+m + AH2 + O2 | - |
Thermothelomyces thermophilus | [(1->4)-beta-D-glucosyl]m-1-(1->4)-D-glucono-1,5-lactone + [(1->4)-beta-D-glucosyl]n + A + H2O | - |
? | |
1.14.99.54 | [(1->4)-beta-D-glucosyl]n+m + AH2 + O2 | - |
Thermothelomyces thermophilus C1 | [(1->4)-beta-D-glucosyl]m-1-(1->4)-D-glucono-1,5-lactone + [(1->4)-beta-D-glucosyl]n + A + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.99.54 | LPMO | ambiguous | Thermothelomyces thermophilus |
1.14.99.54 | LPMO9A | - |
Thermothelomyces thermophilus |
1.14.99.54 | lytic polysaccharide monooxygenase | ambiguous | Thermothelomyces thermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.99.54 | 50 | - |
assay temperature | Thermothelomyces thermophilus |