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Literature summary extracted from

  • Steffler, F.; Guterl, J.K.; Sieber, V.
    Improvement of thermostable aldehyde dehydrogenase by directed evolution for application in Synthetic Cascade Biomanufacturing (2013), Enzyme Microb. Technol., 53, 307-314.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.89
-
 Thermoplasma acidophilum
1.2.1.89 expressed in Escherichia coli BL21(DE3) cells Thermoplasma acidophilum

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.89 34M/Y399C/S405N the mutations enhance enzyme activity with the cofactor NAD+ by a factor of 55.7 Thermoplasma acidophilum
1.2.1.89 F34L the mutation enhances enzyme activity with the cofactor NAD+ by a factor of 3.2 Thermoplasma acidophilum
1.2.1.89 F34M/D372N/S405N the mutations enhance enzyme activity with the cofactor NAD+ by a factor of 7.2 Thermoplasma acidophilum
1.2.1.89 F34M/D372N/Y399C/S405N the mutations enhance enzyme activity with the cofactor NAD+ by a factor of 35.1 Thermoplasma acidophilum
1.2.1.89 F34M/S405N enhances enzyme activity with the cofactor NAD+ by a factor of eight Thermoplasma acidophilum
1.2.1.89 F34M/S405N the mutations enhance enzyme activity with the cofactor NAD+ by a factor of 8.5 while impacts on enzyme stability and substrate specificity are negligible Thermoplasma acidophilum
1.2.1.89 F34M/W271R/S405N the mutations enhance enzyme activity with the cofactor NAD+ by a factor of 22.6 Thermoplasma acidophilum
1.2.1.89 F34M/W271S/S405N the mutations enhance enzyme activity with the cofactor NAD+ by a factor of 42.5 Thermoplasma acidophilum
1.2.1.89 F34M/W271S/Y399C/S405N the mutations enhance enzyme activity with the cofactor NAD+ by a factor of 1.1 Thermoplasma acidophilum
1.2.1.89 F34M/Y399C/S405N enhances enzyme activity with the cofactor NAD+ by a factor of eight and enhances solubilty Thermoplasma acidophilum
1.2.1.89 S405C the mutation enhances enzyme activity with the cofactor NAD+ by a factor of 6 Thermoplasma acidophilum
1.2.1.89 S405N the mutation enhances enzyme activity with the cofactor NAD+ by a factor of 7.4 Thermoplasma acidophilum
1.2.1.89 W271S the mutations enhance enzyme activity with the cofactor NAD+ by a factor of 17.1 Thermoplasma acidophilum
1.2.1.89 Y399C enhances the protein solubility after recombinant expression in Escherichia coli 6fold Thermoplasma acidophilum
1.2.1.89 Y399C the mutation enhances the protein solubility after recombinant expression in Escherichia coli 6fold and enhances enzyme activity with the cofactor NAD+ by a factor of 6.4 Thermoplasma acidophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.89 0.021
-
 NADP+ wild-type, pH 7.0, 50°C Thermoplasma acidophilum
1.2.1.89 0.031
-
 NADP+ mutant F34M/Y399C/S405N, pH 7.0, 50°C Thermoplasma acidophilum
1.2.1.89 0.036
-
 NADP+ mutant F34M/S405N, pH 7.0, 50°C Thermoplasma acidophilum
1.2.1.89 16.7
-
 NAD+ mutant F34M/Y399C/S405N, pH 7.0, 50°C Thermoplasma acidophilum
1.2.1.89 16.7
-
 NAD+ mutant enzyme F34M/Y399C/S405N, at pH 7.0 and 50°C Thermoplasma acidophilum
1.2.1.89 17.6
-
 NAD+ mutant F34M/S405N, pH 7.0, 50°C Thermoplasma acidophilum
1.2.1.89 17.6
-
 NAD+ mutant enzyme F34M/S405N, at pH 7.0 and 50°C Thermoplasma acidophilum
1.2.1.89 22.7
-
 NAD+ wild-type, pH 7.0, 50°C Thermoplasma acidophilum
1.2.1.89 22.7
-
 NAD+ wild type enzyme, at pH 7.0 and 50°C Thermoplasma acidophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.89 55000
-
 x * 55000, SDS-PAGE Thermoplasma acidophilum

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.2.1.89 isobutanol wild-type, 50% residual activity at 3.1%, mutant F34M/S405N at 3.6%, mutant F34M/Y399C/S405N at 3.5%, respectively Thermoplasma acidophilum

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.89 Thermoplasma acidophilum Q9HK01
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.89 HiTrap column chromatography Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.89 D-glyceraldehyde + NAD+ NAD+ is a very poor cofactor for wild-type Thermoplasma acidophilum D-glycerate + NADH + H+
-
 ?
1.2.1.89 D-glyceraldehyde + NADP+ + H2O
-
 Thermoplasma acidophilum D-glycerate + NADPH + H+
-
 ?
1.2.1.89 glyceraldehyde + NAD+ + H2O
-
 Thermoplasma acidophilum glycerate + NADH + H+
-
 ?
1.2.1.89 additional information no activity with acetaldehyde and isobutyraldehyde Thermoplasma acidophilum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.89 ? x * 55000, SDS-PAGE Thermoplasma acidophilum

Synonyms

EC Number Synonyms Comment Organism
1.2.1.89 ALDH
-
 Thermoplasma acidophilum
1.2.1.89 Ta0809
-
 Thermoplasma acidophilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.89 1.64
-
 NAD+ wild-type, pH 7.0, 50°C Thermoplasma acidophilum
1.2.1.89 1.64
-
 NAD+ wild type enzyme, at pH 7.0 and 50°C Thermoplasma acidophilum
1.2.1.89 10.54
-
 NAD+ mutant F34M/Y399C/S405N, pH 7.0, 50°C Thermoplasma acidophilum
1.2.1.89 10.54
-
 NAD+ mutant enzyme F34M/Y399C/S405N, at pH 7.0 and 50°C Thermoplasma acidophilum
1.2.1.89 10.99
-
 NAD+ mutant F34M/S405N, pH 7.0, 50°C Thermoplasma acidophilum
1.2.1.89 10.99
-
 NAD+ mutant enzyme F34M/S405N, at pH 7.0 and 50°C Thermoplasma acidophilum

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.89 NAD+
-
 Thermoplasma acidophilum
1.2.1.89 NADH poor cofoactor Thermoplasma acidophilum
1.2.1.89 NADPH NADPH is preferred over NADH Thermoplasma acidophilum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.89 0.07
-
 NAD+ wild-type, pH 7.0, 50°C Thermoplasma acidophilum
1.2.1.89 0.07
-
 NAD+ wild type enzyme, at pH 7.0 and 50°C Thermoplasma acidophilum
1.2.1.89 0.62
-
 NAD+ mutant F34M/S405N, pH 7.0, 50°C Thermoplasma acidophilum
1.2.1.89 0.62
-
 NAD+ mutant enzyme F34M/S405N, at pH 7.0 and 50°C Thermoplasma acidophilum
1.2.1.89 0.63
-
 NAD+ mutant F34M/Y399C/S405N, pH 7.0, 50°C Thermoplasma acidophilum
1.2.1.89 0.63
-
 NAD+ mutant enzyme F34M/Y399C/S405N, at pH 7.0 and 50°C Thermoplasma acidophilum