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Literature summary extracted from

  • Ludwig-Mueller, J.; Juelke, S.; Geiss, K.; Richter, F.; Mithoefer, A.; ?ola, I.; Rusak, G.; Keenan, S.; Bulman, S.
    A novel methyltransferase from the intracellular pathogen Plasmodiophora brassicae methylates salicylic acid (2015), Mol. Plant Pathol., 16, 349-364.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.273 gene PbBSMT, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) codon plus Plasmodiophora brassicae
2.1.1.274 gene PbBSMT, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) codon plus Plasmodiophora brassicae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.273 additional information Plasmodiophora brassicae the enzyme also catalyzes the reaction of salicylate 1-O-methyltransferase, EC 2.1.1.274 ?
-
 ?
2.1.1.273 S-adenosyl-L-methionine + benzoate Plasmodiophora brassicae
-
 S-adenosyl-L-homocysteine + methyl benzoate
-
 ?
2.1.1.274 additional information Plasmodiophora brassicae the enzyme also catalyzes the reaction of benzoic acid carboxyl methyltransferase, EC 2.1.1.273 ?
-
 ?
2.1.1.274 S-adenosyl-L-methionine + salicylate Plasmodiophora brassicae
-
 S-adenosyl-L-homocysteine + methyl salicylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.273 Plasmodiophora brassicae R4I7S9 gene PbBSMT
-
2.1.1.274 Plasmodiophora brassicae R4I7S9 gene PbBSMT
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.273 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) Codon Plus by metal affinity chromatography Plasmodiophora brassicae
2.1.1.274 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) Codon Plus by metal affinity chromatography Plasmodiophora brassicae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.273 0.0072
-
 purified recombinant enzyme, pH and temperature not specified in the publication Plasmodiophora brassicae
2.1.1.274 0.009
-
 purified recombinant enzyme, pH and temperature not specified in the publication Plasmodiophora brassicae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.273 additional information the enzyme also catalyzes the reaction of salicylate 1-O-methyltransferase, EC 2.1.1.274 Plasmodiophora brassicae ?
-
 ?
2.1.1.273 S-adenosyl-L-methionine + benzoate
-
 Plasmodiophora brassicae S-adenosyl-L-homocysteine + methyl benzoate
-
 ?
2.1.1.274 additional information the enzyme also catalyzes the reaction of benzoic acid carboxyl methyltransferase, EC 2.1.1.273 Plasmodiophora brassicae ?
-
 ?
2.1.1.274 S-adenosyl-L-methionine + salicylate
-
 Plasmodiophora brassicae S-adenosyl-L-homocysteine + methyl salicylate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.273 benzoic acid/salicylic acid methyltransferase
-
 Plasmodiophora brassicae
2.1.1.273 BSMT
-
 Plasmodiophora brassicae
2.1.1.274 benzoic acid/salicylic acid methyltransferase
-
 Plasmodiophora brassicae
2.1.1.274 BSMT
-
 Plasmodiophora brassicae
2.1.1.274 SA methyltransferase
-
 Plasmodiophora brassicae

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.273 S-adenosyl-L-methionine
-
 Plasmodiophora brassicae
2.1.1.274 S-adenosyl-L-methionine
-
 Plasmodiophora brassicae

General Information

EC Number General Information Comment Organism
2.1.1.273 physiological function the obligate biotrophic pathogen Plasmodiophora brassicae causes clubroot disease in Arabidopsis thaliana, which is characterized by large root galls. Salicylic acid production is a defence response in plants, and its methyl ester is involved in systemic signalling. Plasmodiophora brassicae suppresses the plant defence reactions via its methyltransferase, PbBSMT with homology to plant methyltransferases. The PbBSMT gene is maximally transcribed when salicylic acid production is highest, and enzyme PbBSMT can methylate salicylic acid, benzoic and anthranilic acids. Plasmodiophora brassicae secretes enzyme PbBSMT into the host cell, where it methylates the defence signal salicylate. The resulting methyl salicylate fails to upregulate plant defence reactions and is transmitted to leaves, where it is emitted or converted back to salicylate Plasmodiophora brassicae
2.1.1.274 physiological function the obligate biotrophic pathogen Plasmodiophora brassicae causes clubroot disease in Arabidopsis thaliana, which is characterized by large root galls. Salicylic acid production is a defence response in plants, and its methyl ester is involved in systemic signalling. Plasmodiophora brassicae suppresses the plant defence reactions via its methyltransferase, PbBSMT with homology to plant methyltransferases. The PbBSMT gene is maximally transcribed when salicylic acid production is highest, and enzyme PbBSMT can methylate salicylic acid, benzoic and anthranilic acids. Plasmodiophora brassicae secretes enzyme PbBSMT into the host cell, where it methylates the defence signal salicylate. The resulting methyl salicylate fails to upregulate plant defence reactions and is transmitted to leaves, where it is emitted or converted back to salicylate Plasmodiophora brassicae