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Literature summary extracted from

  • Moglia, A.; Lanteri, S.; Comino, C.; Hill, L.; Knevitt, D.; Cagliero, C.; Rubiolo, P.; Bornemann, S.; Martin, C.
    Dual catalytic activity of hydroxycinnamoyl-coenzyme A quinate transferase from tomato allows it to moonlight in the synthesis of both mono- and dicaffeoylquinic acids (2014), Plant Physiol., 166, 1777-1787.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.B33 dithiothreitol slight increase in activity Solanum lycopersicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.B33 expression in Escherichia coli Solanum lycopersicum
2.3.1.99 expression in Escherichia coli Solanum lycopersicum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.B33 homology modeling based on structure 4KEC of Sorghum bicolor hydroxycinnamoyl-coenzyme A transferase Solanum lycopersicum

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.B33 H276Y 58% of wild-type activity for production of dicaffeoylquinic acids, 92% of wild-type hydroxycinnamoyltranferase activity Solanum lycopersicum
2.3.1.99 H276Y 58% of wild-type activity for production of dicaffeoylquinic acids, 92% of wild-type hydroxycinnamoyltranferase activity Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.B33 19
-
 chlorogenic acid pH 4.0, 30°C Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.B33 cytoplasm enzyme catalyzes different reactions in different compartments Solanum lycopersicum 5737
-
2.3.1.B33 vacuole enzyme catalyzes different reactions in different compartments Solanum lycopersicum 5773
-
2.3.1.99 cytoplasm enzyme catalyzes different reactions in different compartments Solanum lycopersicum 5737
-
2.3.1.99 vacuole enzyme catalyzes different reactions in different compartments Solanum lycopersicum 5773
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.B33 Mg2+ slight increase in activity Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.B33 2 chlorogenic acid Solanum lycopersicum reaction is irreversible 1,5-dicaffeoylquinic acid + quinate products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid ir
2.3.1.B33 2 chlorogenic acid Solanum lycopersicum reaction is irreversible 3,5-dicaffeoylquinic acid + quinate products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid ir
2.3.1.B33 2 chlorogenic acid Solanum lycopersicum reaction is irreversible 4,5-dicaffeoylquinic acid + quinate products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid ir

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.B33 Solanum lycopersicum Q70G32 bifunctional enzyme, displays hydroxycinnamoyl-Coenzyme A:quinate hydroxycinnamoyl transferase activity and chlorogenate:chlorogenate transferase activity
-
2.3.1.99 Solanum lycopersicum Q70G32 bifunctional enzyme, displays hydroxycinnamoyl-Coenzyme A:quinate hydroxycinnamoyl transferase activity and chlorogenate:chlorogenate transferase activity
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.B33 fruit increase in CCT activity is observed during ripening, reaching a maximum in the peel of red fruit Solanum lycopersicum
-
2.3.1.99 fruit ripe ftuit Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.B33 22.3
-
 pH 4.0, 30°C Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.B33 2 chlorogenic acid reaction is irreversible Solanum lycopersicum 1,5-dicaffeoylquinic acid + quinate products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid ir
2.3.1.B33 2 chlorogenic acid reaction is irreversible Solanum lycopersicum 3,5-dicaffeoylquinic acid + quinate products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid ir
2.3.1.B33 2 chlorogenic acid reaction is irreversible Solanum lycopersicum 4,5-dicaffeoylquinic acid + quinate products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid ir

Synonyms

EC Number Synonyms Comment Organism
2.3.1.B33 CCT
-
 Solanum lycopersicum
2.3.1.B33 chlorogenate:chlorogenate transferase
-
 Solanum lycopersicum
2.3.1.99 HQT
-
 Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.B33 4
-
-
 Solanum lycopersicum

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.B33 additional information no cofactor required Solanum lycopersicum