EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.1 | synthesis | construction of a synthetic pathway for bioalcohol production at 70°C by insertion of the gene for alcohol dehydrogenase AdhA into the archaeon Pyrococcus furiosus. The engineered strain converts glucose to ethanol via acetate and acetaldehyde, catalyzed by the host-encoded aldehyde ferredoxin oxidoreductase AOR and heterologously expressed AdhA, in an energy-conserving, redox-balanced pathway. The AOR/AdhA pathway also converts exogenously added aliphatic and aromatic carboxylic acids to the corresponding alcohol using glucose, pyruvate, and/or hydrogen as the source of reductant. By heterologous coexpression of a membrane-bound carbon monoxide dehydrogenase, CO is used as a reductant for converting carboxylic acids to alcohols | Thermoanaerobacter sp. |
1.1.1.2 | synthesis | synthetic pathway for bioalcohol production at 70°C by insertion of the gene for bacterial alcohol dehydrogenase AdhA into the archaeon Pyrococcus furiosus. The engineered strain converts glucose to ethanol via acetate and acetaldehyde, catalyzed by the host-encoded aldehyde ferredoxin oxidoreductase AOR and heterologously expressed AdhA, in an energy-conserving, redox-balanced pathway. The AOR/AdhA pathway also converts exogenously added aliphatic and aromatic carboxylic acids to the corresponding alcohol using glucose, pyruvate, and/or hydrogen as the source of reductant. By heterologous coexpression of a membrane-bound carbon monoxide dehydrogenase, CO is used as a reductant for converting carboxylic acids to alcoholsThe AOR/AdhA pathway is a potentially game-changing strategy for syngas fermentation, especially in combination with carbon chain elongation pathways | Thermoanaerobacter sp. |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.1 | - |
Thermoanaerobacter sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.5 | acetate + H+ + reduced ferredoxin | Pyrococcus furiosus | - |
acetaldehyde + H2O + oxidized ferredoxin | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.1 | Thermoanaerobacter sp. | B0K3U7 | - |
- |
1.1.1.2 | Thermoanaerobacter sp. | B0K3U7 | - |
- |
1.2.7.5 | Pyrococcus furiosus | Q51739 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.5 | acetate + H+ + reduced ferredoxin | - |
Pyrococcus furiosus | acetaldehyde + H2O + oxidized ferredoxin | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.1 | iron-containing alcohol dehydrogenase | - |
Thermoanaerobacter sp. |
1.1.1.2 | AdhA | - |
Thermoanaerobacter sp. |
1.1.1.2 | Teth514_0564 | - |
Thermoanaerobacter sp. |
1.2.7.5 | aldehyde Fd oxidoreductase | - |
Pyrococcus furiosus |
1.2.7.5 | AOR | - |
Pyrococcus furiosus |