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Literature summary extracted from

  • Patta, P.; Martinelli, L.; Rotta, M.; Abbadi, B.; Santos, D.; Basso, L.
    Mode of action of recombinant hypoxanthine-guanine phosphoribosyltransferase from Mycobacterium tuberculosis (2015), RSC Adv., 5, 74671-74683.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.8 0.0065
-
 5-phospho-alpha-D-ribose 1-diphosphate with guanine as cosubstrate, at pH 7.5 and 25°C Mycobacterium tuberculosis
2.4.2.8 0.01
-
 guanine at pH 7.5 and 25°C Mycobacterium tuberculosis
2.4.2.8 0.0141
-
 5-phospho-alpha-D-ribose 1-diphosphate with hypoxanthine as cosubstrate, at pH 7.5 and 25°C Mycobacterium tuberculosis
2.4.2.8 0.026
-
 hypoxanthine at pH 7.5 and 25°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.8 Mg2+ required for activity Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.8 22251
-
 2 * 22251, calculated from amino acid sequence Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate Mycobacterium tuberculosis
-
 GMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Mycobacterium tuberculosis
-
 IMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.8 Mycobacterium tuberculosis P9WHQ9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.8
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Mycobacterium tuberculosis GMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Mycobacterium tuberculosis IMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.8 homodimer 2 * 22251, calculated from amino acid sequence Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.4.2.8 HGPRT
-
 Mycobacterium tuberculosis
2.4.2.8 hypoxanthine-guanine phosphoribosyltransferase
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.8 0.193
-
 guanine at pH 7.5 and 25°C Mycobacterium tuberculosis
2.4.2.8 0.89
-
 hypoxanthine at pH 7.5 and 25°C Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.8 0.29
-
 5-phospho-alpha-D-ribose 1-diphosphate with guanine as cosubstrate, at pH 7.5 and 25°C Mycobacterium tuberculosis
2.4.2.8 0.63
-
 5-phospho-alpha-D-ribose 1-diphosphate with hypoxanthine as cosubstrate, at pH 7.5 and 25°C Mycobacterium tuberculosis
2.4.2.8 19
-
 guanine at pH 7.5 and 25°C Mycobacterium tuberculosis
2.4.2.8 34
-
 hypoxanthine at pH 7.5 and 25°C Mycobacterium tuberculosis