EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.61 | expressed in Escherichia coli BL21 cells | Porphyromonas gingivalis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.61 | 0.15 | - |
succinate semialdehyde | with NADH as cosubstrate, at pH 8.0 and 37°C | Porphyromonas gingivalis | |
1.1.1.61 | 0.41 | - |
NADH | with NADH as cosubstrate, at pH 8.0 and 37°C | Porphyromonas gingivalis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.61 | Mn2+ | most favorable metal ion for enzymatic activity | Porphyromonas gingivalis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.61 | 37000 | - |
1 * 37000, SDS-PAGE | Porphyromonas gingivalis |
1.1.1.61 | 42000 | - |
gel filtration | Porphyromonas gingivalis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.61 | succinate semialdehyde + NADH + H+ | Porphyromonas gingivalis | - |
4-hydroxybutanoate + NAD+ | - |
? | |
1.1.1.61 | succinate semialdehyde + NADH + H+ | Porphyromonas gingivalis ATCC 33277 | - |
4-hydroxybutanoate + NAD+ | - |
? | |
1.1.1.61 | succinate semialdehyde + NADPH + H+ | Porphyromonas gingivalis | - |
4-hydroxybutanoate + NADP+ | - |
? | |
1.1.1.61 | succinate semialdehyde + NADPH + H+ | Porphyromonas gingivalis ATCC 33277 | - |
4-hydroxybutanoate + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.61 | Porphyromonas gingivalis | - |
- |
- |
1.1.1.61 | Porphyromonas gingivalis ATCC 33277 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.61 | glutathione Sepharose column chromatography | Porphyromonas gingivalis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.61 | succinate semialdehyde + NADH + H+ | - |
Porphyromonas gingivalis | 4-hydroxybutanoate + NAD+ | - |
? | |
1.1.1.61 | succinate semialdehyde + NADH + H+ | - |
Porphyromonas gingivalis ATCC 33277 | 4-hydroxybutanoate + NAD+ | - |
? | |
1.1.1.61 | succinate semialdehyde + NADPH + H+ | - |
Porphyromonas gingivalis | 4-hydroxybutanoate + NADP+ | - |
? | |
1.1.1.61 | succinate semialdehyde + NADPH + H+ | - |
Porphyromonas gingivalis ATCC 33277 | 4-hydroxybutanoate + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.61 | monomer | 1 * 37000, SDS-PAGE | Porphyromonas gingivalis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.61 | PGN 0724 protein | - |
Porphyromonas gingivalis |
1.1.1.61 | succinate semialdehyde reductase | - |
Porphyromonas gingivalis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.61 | 1329 | - |
succinate semialdehyde | with NADH as cosubstrate, at pH 8.0 and 37°C | Porphyromonas gingivalis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.61 | 8 | - |
- |
Porphyromonas gingivalis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.61 | 7 | 9 | more than 80% activity between pH 7.0 and 9.0 | Porphyromonas gingivalis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.61 | NADH | NADH is a more favorable cofactor for the enzyme than NADPH | Porphyromonas gingivalis | |
1.1.1.61 | NADPH | NADH is a more favorable cofactor for the enzyme than NADPH | Porphyromonas gingivalis |