Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lv, Y.; Kandale, A.; Wun, S.J.; McGeary, R.P.; Williams, S.J.; Kobe, B.; Sieber, V.; Schembri, M.A.; Schenk, G.; Guddat, L.W.
    Crystal structure of Mycobacterium tuberculosis ketol-acid reductoisomerase at 1.0 A resolution - a potential target for anti-tuberculosis drug discovery (2016), FEBS J., 283, 1184-1196.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.86 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.86 native enzyme with two magnesium ions bound in the active site, hanging drop vapor diffusion method, using 250 mM MgCl2, 20% (w/v) PEG 3350, 100 mM Tris/HCl, pH 8.0 Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.86 N-Hydroxy-N-isopropyloxamate
-
 Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.86 0.11
-
 (S)-2-acetolactate at pH 8.5 and 37°C Mycobacterium tuberculosis
1.1.1.86 2.1
-
 3-hydroxypyruvate at pH 8.5 and 37°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.86 Mg2+ dependent on Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.86 72000
-
 multi-angle light scattering Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.86 (S)-2-acetolactate + NADPH + H+ Mycobacterium tuberculosis
-
 (R)-2,3-dihydroxyisovalerate + NADP+
-
 r
1.1.1.86 (S)-2-acetolactate + NADPH + H+ Mycobacterium tuberculosis ATCC 25618
-
 (R)-2,3-dihydroxyisovalerate + NADP+
-
 r
1.1.1.86 2-aceto-2-hydroxybutyrate + NADPH + H+ Mycobacterium tuberculosis
-
 ? + NADP+
-
 r
1.1.1.86 2-aceto-2-hydroxybutyrate + NADPH + H+ Mycobacterium tuberculosis ATCC 25618
-
 ? + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.86 Mycobacterium tuberculosis P9WKJ7
-
-
1.1.1.86 Mycobacterium tuberculosis ATCC 25618 P9WKJ7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.86 Ni-NTA-agarose resin column chromatography and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.86 (S)-2-acetolactate + NADPH + H+
-
 Mycobacterium tuberculosis (R)-2,3-dihydroxyisovalerate + NADP+
-
 r
1.1.1.86 (S)-2-acetolactate + NADPH + H+
-
 Mycobacterium tuberculosis ATCC 25618 (R)-2,3-dihydroxyisovalerate + NADP+
-
 r
1.1.1.86 2-aceto-2-hydroxybutyrate + NADPH + H+
-
 Mycobacterium tuberculosis ? + NADP+
-
 r
1.1.1.86 2-aceto-2-hydroxybutyrate + NADPH + H+
-
 Mycobacterium tuberculosis ATCC 25618 ? + NADP+
-
 r
1.1.1.86 3-hydroxypyruvate + NADPH + H+
-
 Mycobacterium tuberculosis ? + NADP+
-
 r
1.1.1.86 3-hydroxypyruvate + NADPH + H+
-
 Mycobacterium tuberculosis ATCC 25618 ? + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.86 homodimer 2 * 37155, mass spectrometry Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.86 KARI
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.86 1.4
-
 (S)-2-acetolactate at pH 8.5 and 37°C Mycobacterium tuberculosis
1.1.1.86 12.5
-
 3-hydroxypyruvate at pH 8.5 and 37°C Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.86 NADPH
-
 Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.86 0.0000974
-
 N-Hydroxy-N-isopropyloxamate at pH 8.5 and 37°C Mycobacterium tuberculosis