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Literature summary extracted from

  • Gharbia, S.; Shah, H.
    Isolation, purification and characterisation of 2-oxoglutarate reductase from Fusobacterium nucleatum (1991), FEMS Microbiol. Lett., 80, 283-288.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.399 2,2'-dipyridyl disulfide
-
 Fusobacterium nucleatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.399 0.000004
-
 NADH at pH 7.5 and 25°C Fusobacterium nucleatum
1.1.1.399 0.000064
-
 2-oxoglutarate at pH 7.5 and 25°C Fusobacterium nucleatum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.399 68000
-
 x * 68000, SDS-PAGE Fusobacterium nucleatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.399 (R)-2-hydroxyglutarate + NAD+ Fusobacterium nucleatum
-
 2-oxoglutarate + NADH + H+
-
 r
1.1.1.399 2-oxoglutarate + NADH + H+ Fusobacterium nucleatum
-
 (R)-2-hydroxyglutarate + NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.399 Fusobacterium nucleatum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.399 Sepharose-4B-(glutathione-2-pyridyl disulfide) column chromatography Fusobacterium nucleatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.399 (R)-2-hydroxyglutarate + NAD+
-
 Fusobacterium nucleatum 2-oxoglutarate + NADH + H+
-
 r
1.1.1.399 2-oxoglutarate + NADH + H+
-
 Fusobacterium nucleatum (R)-2-hydroxyglutarate + NAD+
-
 r
1.1.1.399 additional information no activity with oxaloacetate, pyruvate, 2-oxobutyrate and glyoxylate Fusobacterium nucleatum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.399 ? x * 68000, SDS-PAGE Fusobacterium nucleatum

Synonyms

EC Number Synonyms Comment Organism
1.1.1.399 serA
-
 Fusobacterium nucleatum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.399 45 60 the enzyme is stable at 45°C for 10 min, 70% of its activity is lost at 50°C, 5% of the original activity is retained at 55°C, while incubation at 60°C abolishes all activity Fusobacterium nucleatum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.399 NADH the enzyme is almost entirely specific towards NADH Fusobacterium nucleatum