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Literature summary extracted from

  • Barbar, A.; Couture, M.; Sen, S.E.; Beliveau, C.; Nisole, A.; Bipfubusa, M.; Cusson, M.
    Cloning, expression and characterization of an insect geranylgeranyl diphosphate synthase from Choristoneura fumiferana (2013), Insect Biochem. Mol. Biol., 43, 947-958.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.29 DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3) Choristoneura fumiferana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.29 (2E,6E)-farnesyl diphosphate the enzyme shows substrate inhibition by its FPP substrate at low IPP concentration, suggesting the existence of a mechanism that may regulate intracellular FPP pools Choristoneura fumiferana
2.5.1.29 geranylgeranyl diphosphate the enzyme shows product inhibition Choristoneura fumiferana
2.5.1.29 Mn2+ required, activates up to 5 mM, inhibitory above 5 mM Choristoneura fumiferana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.29 additional information
-
 additional information Michaelis-Menten kinetics Choristoneura fumiferana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.29 Mg2+ required, activates Choristoneura fumiferana
2.5.1.29 Mn2+ required, activates up to 5 mM, inhibitory above 5 mM Choristoneura fumiferana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.29 (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate Choristoneura fumiferana
-
 diphosphate + geranylgeranyl diphosphate
-
 ?
2.5.1.29 additional information Choristoneura fumiferana enzyme geranylgeranyl diphosphate synthase catalyzes the condensation of the non-allylic diphosphate, isopentenyl diphosphate, with allylic diphosphates to generate a C20 prenyl chain ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.29 Choristoneura fumiferana U3RD44
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.29 recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by metal affinity chromatography Choristoneura fumiferana

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.29 (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate the condensation of isopentenyl diphosphate to (2E,6E)-farnesyl diphosphate by GGPPS is a bi-substrate/biproduct (bi-bi) reaction, where the two products are geranylgeranyl diphosphate and diphosphate, which is cleaved from (2E,6E)-farnesyl diphosphate. The inhibitory pattern observed by geranylgeranyl diphosphate indicates a compulsory ordered mechanism for isopentenyl diphosphate and (2E,6E)-farnesyl diphosphate binding the enzyme from Choristoneura fumiferana Choristoneura fumiferana
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.29 (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Choristoneura fumiferana diphosphate + geranylgeranyl diphosphate
-
 ?
2.5.1.29 (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate preferred reaction Choristoneura fumiferana diphosphate + geranylgeranyl diphosphate
-
 ?
2.5.1.29 dimethylallyl diphosphate + 2 isopentenyl diphosphate the enzyme also performs the synthesis of farnesyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate, reaction of EC 2.5.1.92 Choristoneura fumiferana 2 diphosphate + (2Z,6Z)-farnesyl diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + isopentenyl diphosphate the enzyme also performs the synthesis of farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate, reaction of EC 2.5.1.10. The enzyme prefers farnesyl diphosphate over other allylic substrates for coupling with IPP, it behaves like a type-III geranylgeranyl diphosphate synthase. Ligand binding strutcure, overview Choristoneura fumiferana ?
-
 ?
2.5.1.29 additional information enzyme geranylgeranyl diphosphate synthase catalyzes the condensation of the non-allylic diphosphate, isopentenyl diphosphate, with allylic diphosphates to generate a C20 prenyl chain Choristoneura fumiferana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.29 GGPPS
-
 Choristoneura fumiferana
2.5.1.29 type-III GGPPS
-
 Choristoneura fumiferana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.29 37
-
-
 Choristoneura fumiferana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.29 7
-
-
 Choristoneura fumiferana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.29 0.0165
-
 (2E,6E)-farnesyl diphosphate pH 7.0, 37°C, recombinant enzyme Choristoneura fumiferana

General Information

EC Number General Information Comment Organism
2.5.1.29 additional information molecular homology modeling with CfGGPPS as model sequence and PDB entries 2E8V and 2Q80 (yeast and human GGPPSs) as templates, ligand binding analysis, overview Choristoneura fumiferana
2.5.1.29 physiological function enzyme geranylgeranyl diphosphate synthase catalyzes the condensation of the non-allylic diphosphate, isopentenyl diphosphate, with allylic diphosphates to generate the C20 prenyl chain used for protein prenylation and diterpenoid biosynthesis Choristoneura fumiferana