Literature summary extracted from

Liu, S.P.; Liu, R.X.; El-Rotail, A.A.; Ding, Z.Y.; Gu, Z.H.; Zhang, L.; Shi, G.Y.
Heterologous pathway for the production of L-phenylglycine from glucose by E. coli (2014), J. Biotechnol., 186, 91-97.

Application

EC Number	Application	Comment	Organism
2.6.1.B17	synthesis	engineering of Escherichia coli for biosynthesis of L-phenylglycine from glucose. The enzymes HmaS (L-4-hydroxymandelate synthase), Hmo (L-4-hydroxymandelate oxidase) and HpgT (L-4-hydroxyphenylglycine transaminase) are heterologously expressed in Escherichia coli. HpgT conversing phenylglyoxylate to L-phenylglycine uses an unusual aminodonor L-phenylalanine, which releases another phenylpyruvate as the substrate of HmaS. A recycle-reaction maximizes the utilization of precursor phenylpyruvate. After deletionof tyrB and aspC, L-phenylglycine yield increases by 12.6fold. The L-phenylglycine yield is further improved by 14.9fold after enhancing hmaS expression	Amycolatopsis orientalis

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.6.1.B17	Amycolatopsis orientalis	O52815	cf. EC 2.6.1.103	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.6.1.B17	phenylglyoxylate + L-phenylalanine	-	Amycolatopsis orientalis	L-phenylglycine + phenylpyruvate	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
2.6.1.B17	HpgT	-	Amycolatopsis orientalis
2.6.1.B17	L-4-hydroxyphenylglycine transaminase	-	Amycolatopsis orientalis

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Release 2023.1 (January 2023)