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Literature summary extracted from

  • Slot Christiansen, L.; Munch-Petersen, B.; Knecht, W.
    Non-viral deoxyribonucleoside kinases - diversity and practical use (2015), J. Genet. Genomics, 42, 235-248.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.1.145 medicine suicide gene dmdnk in several cancer cell lines, overview. In vivo testing in a xenograft model for breast cancer, both wild-type DmdNK and mutants of DmdNK are combined with gemcitabine (dFdC, 2',2'-difluoro-deoxycytidine) as the NA prodrug and a positive effect of decreasing tumor volume and increased survival time Drosophila melanogaster
2.7.1.145 additional information DmdNK broad substrate specificity and fast turnover rate make it an interesting candidate for suicide gene therapy, as well as in numerous biotechnological applications Drosophila melanogaster

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.145 crystal structure determination, PDB IDs 1OE0, 1OT3, 1J90, 1ZM7, 1ZMX, 2JCS, 2VP0, 2VP2, 2VP4, 2VP, 2VP6, 2VP9, 2VPP, 2VQ, and 2JJ8 Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.145 cytosol
-
 Bombyx mori 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.145 Mg2+ required Anopheles gambiae
2.7.1.145 Mg2+ required Drosophila melanogaster
2.7.1.145 Mg2+ required Bombyx mori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.145 ATP + 2'-deoxyadenosine Anopheles gambiae
-
 ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyadenosine Drosophila melanogaster
-
 ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyadenosine Bombyx mori
-
 ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxycytidine Anopheles gambiae
-
 ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxycytidine Drosophila melanogaster
-
 ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxycytidine Bombyx mori
-
 ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyguanosine Anopheles gambiae
-
 ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyguanosine Drosophila melanogaster
-
 ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyguanosine Bombyx mori
-
 ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyribonucleoside Anopheles gambiae
-
 ADP + 2'-deoxyribonucleoside 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyribonucleoside Drosophila melanogaster
-
 ADP + 2'-deoxyribonucleoside 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyribonucleoside Bombyx mori
-
 ADP + 2'-deoxyribonucleoside 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxythymidine Anopheles gambiae
-
 ADP + 2'-deoxythymidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxythymidine Drosophila melanogaster
-
 ADP + 2'-deoxythymidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxythymidine Bombyx mori
-
 ADP + 2'-deoxythymidine 5'-phosphate
-
 ?
2.7.1.145 additional information Bombyx mori the multisubstrate kinase from Bombyx mori (BmdNK) prefers pyrimidines ?
-
 ?
2.7.1.145 additional information Drosophila melanogaster the multisubstrate kinase from Drosophila prefers pyrimidines ?
-
 ?
2.7.1.145 additional information Anopheles gambiae the multisubstrate kinase from from Anopheles gambiae (AgdNK) prefers purines ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.145 Anopheles gambiae Q86LB8 gene dnk
-
2.7.1.145 Bombyx mori Q9BKL3 gene dnk
-
2.7.1.145 Drosophila melanogaster Q9XZT6 gene dnk
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.145 ATP + 2'-deoxyadenosine
-
 Anopheles gambiae ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyadenosine
-
 Drosophila melanogaster ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyadenosine
-
 Bombyx mori ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyadenosine 1% of the activity with 2'-deoxythymidine Drosophila melanogaster ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyadenosine 2.7% of the activity with 2'-deoxycytidine Bombyx mori ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyadenosine 48% of the activity with 2'-deoxythymidine Anopheles gambiae ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxycytidine
-
 Anopheles gambiae ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxycytidine
-
 Drosophila melanogaster ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxycytidine
-
 Bombyx mori ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxycytidine best substrate Bombyx mori ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxycytidine 88% of the activity with 2'-deoxythymidine Drosophila melanogaster ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxycytidine 99% of the activity with 2'-deoxythymidine Anopheles gambiae ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyguanosine
-
 Anopheles gambiae ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyguanosine
-
 Drosophila melanogaster ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyguanosine
-
 Bombyx mori ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyguanosine 0.2% of the activity with 2'-deoxythymidine Drosophila melanogaster ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyguanosine 0.5% of the activity with 2'-deoxycytidine Bombyx mori ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyguanosine 83% of the activity with 2'-deoxythymidine Anopheles gambiae ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyribonucleoside
-
 Anopheles gambiae ADP + 2'-deoxyribonucleoside 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyribonucleoside
-
 Drosophila melanogaster ADP + 2'-deoxyribonucleoside 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyribonucleoside
-
 Bombyx mori ADP + 2'-deoxyribonucleoside 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxythymidine
-
 Anopheles gambiae ADP + 2'-deoxythymidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxythymidine
-
 Drosophila melanogaster ADP + 2'-deoxythymidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxythymidine
-
 Bombyx mori ADP + 2'-deoxythymidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxythymidine best substrate Anopheles gambiae ADP + 2'-deoxythymidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxythymidine best substrate Drosophila melanogaster ADP + 2'-deoxythymidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxythymidine 65% of the activity with 2'-deoxycytidine Bombyx mori ADP + 2'-deoxythymidine 5'-phosphate
-
 ?
2.7.1.145 additional information the multisubstrate kinase from Bombyx mori (BmdNK) prefers pyrimidines Bombyx mori ?
-
 ?
2.7.1.145 additional information the multisubstrate kinase from Drosophila prefers pyrimidines Drosophila melanogaster ?
-
 ?
2.7.1.145 additional information the multisubstrate kinase from from Anopheles gambiae (AgdNK) prefers purines Anopheles gambiae ?
-
 ?
2.7.1.145 additional information substrate binding of non-TK1-like dNKs, overview Anopheles gambiae ?
-
 ?
2.7.1.145 additional information substrate binding of non-TK1-like dNKs, overview Drosophila melanogaster ?
-
 ?
2.7.1.145 additional information substrate binding of non-TK1-like dNKs, overview Bombyx mori ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.145 AgdNK
-
 Anopheles gambiae
2.7.1.145 BmdNK
-
 Bombyx mori
2.7.1.145 DmdNK
-
 Drosophila melanogaster
2.7.1.145 dNK
-
 Anopheles gambiae
2.7.1.145 dNK
-
 Drosophila melanogaster
2.7.1.145 dNK
-
 Bombyx mori

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.145 ATP
-
 Anopheles gambiae
2.7.1.145 ATP
-
 Drosophila melanogaster
2.7.1.145 ATP
-
 Bombyx mori

General Information

EC Number General Information Comment Organism
2.7.1.145 evolution evolution of dNKs, overview. The two families of dNKs, the TK1-like kinases and the non-TK1-like kinases, have overall unique monomer structures and only share significant protein sequence similarity at the Ploop, a glycine rich ATP binding domain close to the N-terminus. This suggests that the two families have different evolutionary origins. The insect dNK is a non-TK1 like kinase Anopheles gambiae
2.7.1.145 evolution evolution of dNKs, overview. The two families of dNKs, the TK1-like kinases and the non-TK1-like kinases, have overall unique monomer structures and only share significant protein sequence similarity at the Ploop, a glycine rich ATP binding domain close to the N-terminus. This suggests that the two families have different evolutionary origins. The insect dNK is a non-TK1 like kinase Drosophila melanogaster
2.7.1.145 evolution evolution of dNKs, overview. The two families of dNKs, the TK1-like kinases and the non-TK1-like kinases, have overall unique monomer structures and only share significant protein sequence similarity at the Ploop, a glycine rich ATP binding domain close to the N-terminus. This suggests that the two families have different evolutionary origins. The insect dNK is a non-TK1 like kinase Bombyx mori
2.7.1.145 additional information structure of non-TK1 like kinases, overview Anopheles gambiae
2.7.1.145 additional information structure of non-TK1 like kinases, overview Drosophila melanogaster
2.7.1.145 additional information structure of non-TK1 like kinases, overview Bombyx mori
2.7.1.145 physiological function DmdNK is cell cycle regulated and under the control of cyclin E and the dE2F1 transcription factor, involved in G1/S phase transition Drosophila melanogaster