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Literature summary extracted from

  • Nyati, P.; Rivera-Perez, C.; Noriega, F.G.
    Negative feedbacks by isoprenoids on a mevalonate kinase expressed in the corpora allata of mosquitoes (2015), PLoS ONE, 10, e0143107.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.36 expressed in Escherichia coli Aedes aegypti

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.36 farnesyl diphosphate
-
 Aedes aegypti
2.7.1.36 geranyl diphosphate
-
 Aedes aegypti
2.7.1.36 geranylgeranyl diphosphate
-
 Aedes aegypti
2.7.1.36 additional information not inhibited by by isopentenyl diphosphate and dimethylallyl diphosphate Aedes aegypti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.36 0.09
-
 (R)-mevalonate at pH 7.5, temperature not specified in the publication Aedes aegypti
2.7.1.36 0.14
-
 ATP at pH 7.5, temperature not specified in the publication Aedes aegypti

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.36 Co2+ Co2+ enhances enzyme to a lesser degree than Mg2+ Aedes aegypti
2.7.1.36 Mg2+ in the presence of Mg2+, the enzyme has higher affinity for (R)-mevalonate than ATP Aedes aegypti
2.7.1.36 Mn2+ Mn2+ enhances enzyme to a lesser degree than Mg2+ Aedes aegypti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.36 43270
-
 x * 43270, calculated from amino acid sequence Aedes aegypti

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.36 ATP + (R)-mevalonate Aedes aegypti 100% activity with ATP ADP + (R)-5-phosphomevalonate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.36 Aedes aegypti
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.36 HiTrap affinity column chromatography Aedes aegypti

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.36 corpus allatum
-
 Aedes aegypti
-
2.7.1.36 thorax
-
 Aedes aegypti
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.36 ATP + (R)-mevalonate 100% activity with ATP Aedes aegypti ADP + (R)-5-phosphomevalonate
-
 ?
2.7.1.36 CTP + (R)-mevalonate 5% activity with GTP compared to ATP Aedes aegypti CDP + (R)-5-phosphomevalonate
-
 ?
2.7.1.36 GTP + (R)-mevalonate 15% activity with GTP compared to ATP Aedes aegypti GDP + (R)-5-phosphomevalonate
-
 ?
2.7.1.36 TTP + (R)-mevalonate 11% activity with GTP compared to ATP Aedes aegypti TDP + (R)-5-phosphomevalonate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.36 ? x * 43270, calculated from amino acid sequence Aedes aegypti

Synonyms

EC Number Synonyms Comment Organism
2.7.1.36 MVK
-
 Aedes aegypti

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.36 10.1
-
 ATP at pH 7.5, temperature not specified in the publication Aedes aegypti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.36 7.5 8
-
 Aedes aegypti

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.36 0.00044
-
 farnesyl diphosphate at pH 7.5, temperature not specified in the publication Aedes aegypti
2.7.1.36 0.00055
-
 geranyl diphosphate at pH 7.5, temperature not specified in the publication Aedes aegypti
2.7.1.36 0.00093
-
 geranylgeranyl diphosphate at pH 7.5, temperature not specified in the publication Aedes aegypti

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.36 Aedes aegypti calculated from amino acid sequence
-
 5.8