EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.4 | expression in Escherichia coli | Clostridium autoethanogenum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.4 | G198D/S199 V/P201E/Y218A | mutant displays no detectable activity with NADPH as cofactor, but uses NADH with kcat/Km value of 12 per s and mM | Clostridium autoethanogenum |
1.1.1.4 | additional information | variation of residue S199 does not yield any NADH-dependent enzymes | Clostridium autoethanogenum |
1.1.1.4 | S199A | increase in catalytic efficiencies | Clostridium autoethanogenum |
1.1.1.4 | S199C | decrease in catalytic efficiencies, with no significant changes in substrate preference | Clostridium autoethanogenum |
1.1.1.4 | S199G | decrease in catalytic efficiencies, with no significant changes in substrate preference | Clostridium autoethanogenum |
1.1.1.4 | S199R | increase in catalytic efficiencies | Clostridium autoethanogenum |
1.1.1.4 | S199W | decrease in catalytic efficiencies, with no significant changes in substrate preference | Clostridium autoethanogenum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.4 | 0.0017 | - |
NADPH | wild-type, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 0.0056 | - |
NADPH | mutant S199A, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 0.036 | - |
NADPH | mutant S199R, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 0.8 | - |
2-butanone | mutant S199A, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 0.9 | - |
acetone | mutant S199A, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 1 | - |
acetone | wild-type, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 1.1 | - |
acetone | mutant S199R, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 1.2 | - |
acetone | mutant S199C, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 1.5 | - |
2-butanone | mutant S199C, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 1.6 | - |
acetone | mutant S199G, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 1.8 | - |
2-butanone | wild-type, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 1.9 | - |
2-butanone | mutant S199R, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 2 | - |
acetone | mutant S199W, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 2.4 | - |
2-butanone | mutant S199G, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 3.6 | - |
2-butanone | mutant S199W, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 34 | - |
(R)-acetoin | mutant S199R, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 45 | - |
(R)-acetoin | mutant S199C, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 98 | - |
(R)-acetoin | wild-type, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 120 | - |
(R)-acetoin | mutant S199A, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 190 | - |
(R)-acetoin | mutant S199W, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 230 | - |
(R)-acetoin | mutant S199G, pH 7.5, 25°C | Clostridium autoethanogenum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.4 | Clostridium autoethanogenum | F8TEL7 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.4 | (R)-acetoin + NADPH + H+ | - |
Clostridium autoethanogenum | (2R,3R)-2,3-butanediol + NAD+ | - |
? | |
1.1.1.4 | 2-butanone + NADP+ | - |
Clostridium autoethanogenum | 2-butanol + NADPH + H+ | - |
? | |
1.1.1.4 | acetone + NADPH + H+ | - |
Clostridium autoethanogenum | propan-2-ol + NADP+ | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.4 | 2 | 8 | 2-butanone | mutant S199W, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 3 | 6 | acetone | mutant S199C, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 23 | - |
2-butanone | mutant S199C, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 32 | - |
NADPH | mutant S199R, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 37 | - |
2-butanone | wild-type, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 40 | - |
2-butanone | mutant S199G, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 41 | - |
(R)-acetoin | mutant S199C, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 46 | - |
2-butanone | mutant S199A, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 48 | - |
acetone | mutant S199W, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 50 | - |
NADPH | mutant S199A, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 50 | - |
2-butanone | mutant S199R, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 55 | - |
acetone | wild-type, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 60 | - |
acetone | mutant S199A, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 62 | - |
acetone | mutant S199G, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 66 | - |
(R)-acetoin | mutant S199W, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 68 | - |
NADPH | wild-type, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 89 | - |
acetone | mutant S199R, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 95 | - |
(R)-acetoin | mutant S199R, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 110 | - |
(R)-acetoin | mutant S199G, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 150 | - |
(R)-acetoin | wild-type, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 230 | - |
(R)-acetoin | mutant S199A, pH 7.5, 25°C | Clostridium autoethanogenum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.4 | NADH | cofoactor of mutant G198D/S199 V/P201E/Y218A | Clostridium autoethanogenum | |
1.1.1.4 | NADPH | strictly dependent on NADPH | Clostridium autoethanogenum |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.4 | 0.35 | - |
(R)-acetoin | mutant S199W, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 0.48 | - |
(R)-acetoin | mutant S199G, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 0.9 | - |
(R)-acetoin | mutant S199C, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 1.6 | - |
(R)-acetoin | wild-type, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 1.9 | - |
(R)-acetoin | mutant S199A, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 2.8 | - |
(R)-acetoin | mutant S199R, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 7.8 | - |
2-butanone | mutant S199W, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 15 | - |
2-butanone | mutant S199C, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 17 | - |
2-butanone | mutant S199G, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 21 | - |
2-butanone | wild-type, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 24 | - |
acetone | mutant S199W, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 26 | - |
2-butanone | mutant S199R, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 30 | - |
acetone | mutant S199C, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 39 | - |
acetone | mutant S199G, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 55 | - |
acetone | wild-type, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 61 | - |
2-butanone | mutant S199A, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 67 | - |
acetone | mutant S199A, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 81 | - |
acetone | mutant S199R, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 890 | - |
NADPH | mutant S199R, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 8900 | - |
NADPH | mutant S199A, pH 7.5, 25°C | Clostridium autoethanogenum | |
1.1.1.4 | 39000 | - |
NADPH | wild-type, pH 7.5, 25°C | Clostridium autoethanogenum |