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Literature summary extracted from
- Monitoring of PQQ-dependent glucose dehydrogenase substrate specificity for its potential use in biocatalysis and bioanalysis (2013), Appl. Biochem. Biotechnol., 171, 1032-1041.
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.5.2 | soluble | - |
Acinetobacter calcoaceticus | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.5.2 | D-glucose + ubiquinone | Acinetobacter calcoaceticus | - |
D-glucono-1,5-lactone + ubiquinol | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.5.2 | Acinetobacter calcoaceticus | - |
- |
- |
| 1.1.99.35 | Acinetobacter calcoaceticus | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.5.2 | cellobiose + ubiquinone | high activity | Acinetobacter calcoaceticus | ? | - |
? | |
| 1.1.5.2 | D-allose + ubiquinone | high activity | Acinetobacter calcoaceticus | ? | - |
? | |
| 1.1.5.2 | D-galactose + ubiquinone | low activity | Acinetobacter calcoaceticus | ? | - |
? | |
| 1.1.5.2 | D-glucose + ubiquinone | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ubiquinol | - |
? | |
| 1.1.5.2 | D-glucose + ubiquinone | highest activity | Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ubiquinol | - |
? | |
| 1.1.5.2 | D-lyxose + ubiquinone | very low activity | Acinetobacter calcoaceticus | ? | - |
? | |
| 1.1.5.2 | D-mannose + ubiquinone | low activity | Acinetobacter calcoaceticus | ? | - |
? | |
| 1.1.5.2 | D-ribose + ubiquinone | low activity | Acinetobacter calcoaceticus | ? | - |
? | |
| 1.1.5.2 | D-xylose + ubiquinone | low activity | Acinetobacter calcoaceticus | ? | - |
? | |
| 1.1.5.2 | L-arabinose + ubiquinone | high activity | Acinetobacter calcoaceticus | ? | - |
? | |
| 1.1.5.2 | lactose + ubiquinone | - |
Acinetobacter calcoaceticus | ? | - |
? | |
| 1.1.5.2 | maltose + ubiquinone | - |
Acinetobacter calcoaceticus | ? | - |
? | |
| 1.1.5.2 | melibiose + ubiquinone | low activity | Acinetobacter calcoaceticus | ? | - |
? | |
| 1.1.5.2 | additional information | no activity with D-glucosamine, L-altrose, L-lyxose, L-talose, L-mannose, D-altrose, D-arabinose, L-xylose, L-glucose, L-galactose, D-talose, and L-allose | Acinetobacter calcoaceticus | ? | - |
? | |
| 1.1.99.35 | cellobiose + N-methylphenazonium methylsulfate | - |
Acinetobacter calcoaceticus | cellobiono-1,5-lactone + reduced N-methylphenazonium methylsulfate | - |
? | |
| 1.1.99.35 | D-allose + N-methylphenazonium methylsulfate | - |
Acinetobacter calcoaceticus | D-allono-1,5-lactone + reduced N-methylphenazonium methylsulfate | - |
? | |
| 1.1.99.35 | D-galactose + N-methylphenazonium methylsulfate | 30% activity compared to D-glucose | Acinetobacter calcoaceticus | D-galactono-1,5-lactone + reduced N-methylphenazonium methylsulfate | - |
? | |
| 1.1.99.35 | D-glucose + N-methylphenazonium methylsulfate | 100% activity | Acinetobacter calcoaceticus | D-glucono-1,5-lactone + reduced N-methylphenazonium methylsulfate | - |
? | |
| 1.1.99.35 | D-lyxose + N-methylphenazonium methylsulfate | - |
Acinetobacter calcoaceticus | D-lyxono-1,5-lactone + reduced N-methylphenazonium methylsulfate | - |
? | |
| 1.1.99.35 | D-mannose + N-methylphenazonium methylsulfate | - |
Acinetobacter calcoaceticus | D-mannono-1,5-lactone + reduced N-methylphenazonium methylsulfate | - |
? | |
| 1.1.99.35 | D-ribose + N-methylphenazonium methylsulfate | 5% activity compared to D-glucose | Acinetobacter calcoaceticus | D-ribono-1,5-lactone + reduced N-methylphenazonium methylsulfate | - |
? | |
| 1.1.99.35 | D-xylose + N-methylphenazonium methylsulfate | 14% activity compared to D-glucose | Acinetobacter calcoaceticus | D-xylono-1,5-lactone + reduced N-methylphenazonium methylsulfate | - |
? | |
| 1.1.99.35 | L-arabinose + N-methylphenazonium methylsulfate | - |
Acinetobacter calcoaceticus | L-arabinono-1,5-lactone + reduced N-methylphenazonium methylsulfate | - |
? | |
| 1.1.99.35 | lactose + N-methylphenazonium methylsulfate | 4% activity compared to D-glucose | Acinetobacter calcoaceticus | lactono-1,5-lactone + reduced N-methylphenazonium methylsulfate | - |
? | |
| 1.1.99.35 | maltose + N-methylphenazonium methylsulfate | 12% activity compared to D-glucose | Acinetobacter calcoaceticus | maltono-1,5-lactone + reduced N-methylphenazonium methylsulfate | - |
? | |
| 1.1.99.35 | melibiose + N-methylphenazonium methylsulfate | 5% activity compared to D-glucose | Acinetobacter calcoaceticus | melibiono-1,5-lactone + reduced N-methylphenazonium methylsulfate | - |
? | |
| 1.1.99.35 | additional information | no activity with D-glucosamine, L-altrose, L-lyxose, L-talose, L-mannose, D-altrose, D-arabinose, D-talose, L-xylose, L-glucose, L-galactose, L-allose, sucrose, trehalose, and raffinose | Acinetobacter calcoaceticus | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.5.2 | sGDH-PQQ | - |
Acinetobacter calcoaceticus |
| 1.1.5.2 | soluble PQQ-dependent glucose dehydrogenase | - |
Acinetobacter calcoaceticus |
| 1.1.99.35 | 2,7,9-tricarboxypyrroloquinoline quinone-dependent glucose dehydrogenase | - |
Acinetobacter calcoaceticus |
| 1.1.99.35 | sGDH-PQQ | - |
Acinetobacter calcoaceticus |
| 1.1.99.35 | soluble PQQ-dependent glucose dehydrogenase | - |
Acinetobacter calcoaceticus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.35 | pyrroloquinoline quinone | - |
Acinetobacter calcoaceticus | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.5.2 | 0.24 | - |
D-Lyxose | pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1.1.5.2 | 11 | - |
D-ribose | pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1.1.5.2 | 11 | - |
melibiose | pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1.1.5.2 | 46 | - |
D-xylose | pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1.1.5.2 | 50 | - |
D-mannose | pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1.1.5.2 | 150 | - |
D-galactose | pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1.1.5.2 | 790 | - |
lactose | pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1.1.5.2 | 800 | - |
maltose | pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1.1.5.2 | 1080 | - |
cellobiose | pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1.1.5.2 | 1450 | - |
L-arabinose | pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1.1.5.2 | 1470 | - |
D-allose | pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1.1.5.2 | 1700 | - |
D-glucose | pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1.1.99.35 | 0.24 | - |
D-Lyxose | free enzyme, pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1.1.99.35 | 11 | - |
D-ribose | free enzyme, pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1.1.99.35 | 11 | - |
melibiose | free enzyme, pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1.1.99.35 | 46 | - |
D-xylose | free enzyme, pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1.1.99.35 | 50 | - |
D-mannose | free enzyme, pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1.1.99.35 | 150 | - |
D-galactose | free enzyme, pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1.1.99.35 | 790 | - |
lactose | free enzyme, pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1.1.99.35 | 800 | - |
maltose | free enzyme, pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1.1.99.35 | 1080 | - |
cellobiose | free enzyme, pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1.1.99.35 | 1450 | - |
L-arabinose | free enzyme, pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1.1.99.35 | 1470 | - |
D-allose | free enzyme, pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1.1.99.35 | 1700 | - |
D-glucose | free enzyme, pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
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