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Literature summary extracted from

  • Vick, J.E.; Clomburg, J.M.; Blankschien, M.D.; Chou, A.; Kim, S.; Gonzalez, R.
    Escherichia coli enoyl-acyl carrier protein reductase (FabI) supports efficient operation of a functional reversal of ?-oxidation cycle (2015), Appl. Environ. Microbiol., 81, 1406-1416.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.9 acetoacetyl-CoA about 20% inhibition at 0.2 mM Escherichia coli
1.3.1.9 decanoyl-CoA about 15% inhibition at 0.2 mM Escherichia coli
1.3.1.9 lauroyl-CoA about 50% inhibition at 0.2 mM Escherichia coli
1.3.1.9 additional information not inhibited by 3-hydroxybutyryl-CoA, butyryl-CoA, hexanoyl-CoA, octanoyl-CoA Escherichia coli
1.3.1.9 myristoyl-CoA about 80% inhibition at 0.2 mM Escherichia coli
1.3.1.9 palmitic acid
-
 Escherichia coli
1.3.1.9 palmitoyl-CoA nearly 50% inhibition at 0.02 mM Escherichia coli
1.3.1.9 stearoyl-CoA about 40% inhibition at 0.2 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.9 4.6
-
 crotonyl-CoA at pH 7.5 and 30°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.9 additional information Mg2+ has marginal effects on activity level Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.9 an acyl-[acyl-carrier protein] + NAD+ Escherichia coli
-
 a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.9 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.9 Talon metal affinity resin column chromatography, and Superdex 200 gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.9 an acyl-[acyl-carrier protein] + NAD+
-
 Escherichia coli a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+
-
 ?
1.3.1.9 crotonyl-CoA + NADH + H+
-
 Escherichia coli butyryl-CoA + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.9 enoyl-acyl carrier protein reductase
-
 Escherichia coli
1.3.1.9 ENR
-
 Escherichia coli
1.3.1.9 FabI
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.9 5.3
-
 crotonyl-CoA at pH 7.5 and 30°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.9 8.5
-
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.9 NAD+
-
 Escherichia coli
1.3.1.9 NADH
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.1.9 0.0054
-
 palmitic acid at pH 7.5 and 30°C Escherichia coli
1.3.1.9 0.9
-
 decanoyl-CoA at pH 7.5 and 30°C Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3.1.9 1.15
-
 crotonyl-CoA at pH 7.5 and 30°C Escherichia coli