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Literature summary extracted from

  • An, R.; Moe, L.A.
    Regulation of pyrroloquinoline quinone-dependent glucose dehydrogenase activity in the model rhizosphere-dwelling bacterium Pseudomonas putida KT2440 (2016), Appl. Environ. Microbiol., 82, 4955-4964.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.2 expressed in Escherichia coli DH5alpha cells Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.2 0.0001
-
ubiquinone Km below 0.0002 mM, native enzyme, at pH 7.5 and 28°C Pseudomonas putida
1.1.5.2 0.00113
-
ubiquinone recombinant enzyme, at pH 7.5 and 28°C Pseudomonas putida
1.1.5.2 2.71
-
D-glucose recombinant enzyme, at pH 7.5 and 28°C Pseudomonas putida
1.1.5.2 4.91
-
D-glucose native enzyme, at pH 7.5 and 28°C Pseudomonas putida
1.1.99.35 4.91
-
D-glucose at pH 7.0 and 25°C Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.2 periplasm
-
Pseudomonas putida
-
-
1.1.99.35 periplasm
-
Pseudomonas putida
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.99.35 Ca2+ 0.5 mM used in assay conditions Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.5.2 D-glucose + ubiquinone Pseudomonas putida
-
D-glucono-1,5-lactone + ubiquinol
-
?
1.1.5.2 D-glucose + ubiquinone Pseudomonas putida KT 2240
-
D-glucono-1,5-lactone + ubiquinol
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.2 Pseudomonas putida
-
-
-
1.1.5.2 Pseudomonas putida KT 2240
-
-
-
1.1.99.35 Pseudomonas putida
-
-
-
1.1.99.35 Pseudomonas putida KT 2240
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.2 D-glucose + oxidized 2,6-dichlorophenolindophenol
-
Pseudomonas putida D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
1.1.5.2 D-glucose + oxidized 2,6-dichlorophenolindophenol
-
Pseudomonas putida KT 2240 D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
1.1.5.2 D-glucose + ubiquinone
-
Pseudomonas putida D-glucono-1,5-lactone + ubiquinol
-
?
1.1.5.2 D-glucose + ubiquinone
-
Pseudomonas putida KT 2240 D-glucono-1,5-lactone + ubiquinol
-
?
1.1.99.35 D-glucose + 2,6-dichlorophenolindophenol
-
Pseudomonas putida D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
1.1.99.35 D-glucose + 2,6-dichlorophenolindophenol
-
Pseudomonas putida KT 2240 D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
1.1.99.35 D-glucose + phenazine methosulfate
-
Pseudomonas putida D-glucono-1,5-lactone + reduced phenazine methosulfate
-
?
1.1.99.35 D-glucose + phenazine methosulfate
-
Pseudomonas putida KT 2240 D-glucono-1,5-lactone + reduced phenazine methosulfate
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.5.2 GCD
-
Pseudomonas putida
1.1.5.2 GDH
-
Pseudomonas putida
1.1.99.35 GCD
-
Pseudomonas putida
1.1.99.35 GDH
-
Pseudomonas putida
1.1.99.35 pyrroloquinoline quinone-dependent glucose dehydrogenase
-
Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.35 pyrroloquinoline quinone
-
Pseudomonas putida

Expression

EC Number Organism Comment Expression
1.1.99.35 Pseudomonas putida the gene expression level is approximately 1.5-3fold higher in the zero-soluble-phosphate condition than in high-soluble-phosphate condition. Enzyme expression is highest with D-glucose in the growth medium up