EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.5.2 | expressed in Escherichia coli DH5alpha cells | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.5.2 | 0.0001 | - |
ubiquinone | Km below 0.0002 mM, native enzyme, at pH 7.5 and 28°C | Pseudomonas putida | |
1.1.5.2 | 0.00113 | - |
ubiquinone | recombinant enzyme, at pH 7.5 and 28°C | Pseudomonas putida | |
1.1.5.2 | 2.71 | - |
D-glucose | recombinant enzyme, at pH 7.5 and 28°C | Pseudomonas putida | |
1.1.5.2 | 4.91 | - |
D-glucose | native enzyme, at pH 7.5 and 28°C | Pseudomonas putida | |
1.1.99.35 | 4.91 | - |
D-glucose | at pH 7.0 and 25°C | Pseudomonas putida |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.5.2 | periplasm | - |
Pseudomonas putida | - |
- |
1.1.99.35 | periplasm | - |
Pseudomonas putida | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.35 | Ca2+ | 0.5 mM used in assay conditions | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.5.2 | D-glucose + ubiquinone | Pseudomonas putida | - |
D-glucono-1,5-lactone + ubiquinol | - |
? | |
1.1.5.2 | D-glucose + ubiquinone | Pseudomonas putida KT 2240 | - |
D-glucono-1,5-lactone + ubiquinol | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.5.2 | Pseudomonas putida | - |
- |
- |
1.1.5.2 | Pseudomonas putida KT 2240 | - |
- |
- |
1.1.99.35 | Pseudomonas putida | - |
- |
- |
1.1.99.35 | Pseudomonas putida KT 2240 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.5.2 | D-glucose + oxidized 2,6-dichlorophenolindophenol | - |
Pseudomonas putida | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.5.2 | D-glucose + oxidized 2,6-dichlorophenolindophenol | - |
Pseudomonas putida KT 2240 | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.5.2 | D-glucose + ubiquinone | - |
Pseudomonas putida | D-glucono-1,5-lactone + ubiquinol | - |
? | |
1.1.5.2 | D-glucose + ubiquinone | - |
Pseudomonas putida KT 2240 | D-glucono-1,5-lactone + ubiquinol | - |
? | |
1.1.99.35 | D-glucose + 2,6-dichlorophenolindophenol | - |
Pseudomonas putida | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.35 | D-glucose + 2,6-dichlorophenolindophenol | - |
Pseudomonas putida KT 2240 | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.35 | D-glucose + phenazine methosulfate | - |
Pseudomonas putida | D-glucono-1,5-lactone + reduced phenazine methosulfate | - |
? | |
1.1.99.35 | D-glucose + phenazine methosulfate | - |
Pseudomonas putida KT 2240 | D-glucono-1,5-lactone + reduced phenazine methosulfate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.5.2 | GCD | - |
Pseudomonas putida |
1.1.5.2 | GDH | - |
Pseudomonas putida |
1.1.99.35 | GCD | - |
Pseudomonas putida |
1.1.99.35 | GDH | - |
Pseudomonas putida |
1.1.99.35 | pyrroloquinoline quinone-dependent glucose dehydrogenase | - |
Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.35 | pyrroloquinoline quinone | - |
Pseudomonas putida |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.1.99.35 | Pseudomonas putida | the gene expression level is approximately 1.5-3fold higher in the zero-soluble-phosphate condition than in high-soluble-phosphate condition. Enzyme expression is highest with D-glucose in the growth medium | up |