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Literature summary extracted from

  • Ozawa, K.; Iwasa, H.; Sasaki, N.; Kinoshita, N.; Hiratsuka, A.; Yokoyama, K.
    Identification and characterization of thermostable glucose dehydrogenases from thermophilic filamentous fungi (2017), Appl. Microbiol. Biotechnol., 101, 173-183.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.9 expressed in Escherichia coli and Pichia pastoris Rasamsonia emersonii
1.1.5.9 expressed in Escherichia coli and Pichia pastoris Thermoascus crustaceus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.9 58000
-
 x * 58000, SDS-PAGE Thermoascus crustaceus
1.1.5.9 60000
-
 x * 60000, SDS-PAGE Rasamsonia emersonii

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.9 Rasamsonia emersonii A0A1E1GL61
-
-
1.1.5.9 Rasamsonia emersonii NBRC 31232 A0A1E1GL61
-
-
1.1.5.9 Thermoascus crustaceus A0A1E1GL87
-
-
1.1.5.9 Thermoascus crustaceus NBRC 9129 A0A1E1GL87
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.5.9 glycoprotein
-
 Rasamsonia emersonii
1.1.5.9 glycoprotein
-
 Thermoascus crustaceus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.9 Ni-NTA agarose column chromatography Rasamsonia emersonii
1.1.5.9 Ni-NTA agarose column chromatography Thermoascus crustaceus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.9 2-deoxy-D-glucose + 2,6-dichlorophenolindophenol 11.5% activity compared to D-glucose Rasamsonia emersonii 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 2-deoxy-D-glucose + 2,6-dichlorophenolindophenol 60.1% activity compared to D-glucose Thermoascus crustaceus 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 2-deoxy-D-glucose + 2,6-dichlorophenolindophenol 60.1% activity compared to D-glucose Thermoascus crustaceus NBRC 9129 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 2-deoxy-D-glucose + 2,6-dichlorophenolindophenol 11.5% activity compared to D-glucose Rasamsonia emersonii NBRC 31232 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-glucose + 2,6-dichlorophenolindophenol high substrate specificity for D-glucose Rasamsonia emersonii D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-glucose + 2,6-dichlorophenolindophenol high substrate specificity for D-glucose Thermoascus crustaceus D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-glucose + 2,6-dichlorophenolindophenol high substrate specificity for D-glucose Thermoascus crustaceus NBRC 9129 D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-glucose + 2,6-dichlorophenolindophenol high substrate specificity for D-glucose Rasamsonia emersonii NBRC 31232 D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-xylose + 2,6-dichloroindophenol 24.8% activity compared to D-glucose Rasamsonia emersonii D-xylono-1,5-lactone + 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-xylose + 2,6-dichloroindophenol 24.8% activity compared to D-glucose Rasamsonia emersonii NBRC 31232 D-xylono-1,5-lactone + 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-xylose + 2,6-dichlorophenolindophenol 27.6% activity compared to D-glucose Thermoascus crustaceus D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-xylose + 2,6-dichlorophenolindophenol 27.6% activity compared to D-glucose Thermoascus crustaceus NBRC 9129 D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 maltotriose + 2,6-dichlorophenolindophenol 3.4% activity compared to D-glucose Rasamsonia emersonii maltotriono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 maltotriose + 2,6-dichlorophenolindophenol 6.8% activity compared to D-glucose Thermoascus crustaceus maltotriono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 maltotriose + 2,6-dichlorophenolindophenol 6.8% activity compared to D-glucose Thermoascus crustaceus NBRC 9129 maltotriono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 maltotriose + 2,6-dichlorophenolindophenol 3.4% activity compared to D-glucose Rasamsonia emersonii NBRC 31232 maltotriono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 additional information no activity in the presence of maltose, D-arabinose, D-fructose, D-galactose, maltotetraose, D-mannitol, D-mannose, D-raffinose, D-sorbitol, sucrose, and D-trehalose Rasamsonia emersonii ?
-
 ?
1.1.5.9 additional information no activity in the presence of maltose, D-arabinose, D-fructose, D-galactose, maltotetraose, D-mannitol, D-mannose, D-raffinose, D-sorbitol, sucrose, and D-trehalose Thermoascus crustaceus ?
-
 ?
1.1.5.9 additional information no activity in the presence of maltose, D-arabinose, D-fructose, D-galactose, maltotetraose, D-mannitol, D-mannose, D-raffinose, D-sorbitol, sucrose, and D-trehalose Thermoascus crustaceus NBRC 9129 ?
-
 ?
1.1.5.9 additional information no activity in the presence of maltose, D-arabinose, D-fructose, D-galactose, maltotetraose, D-mannitol, D-mannose, D-raffinose, D-sorbitol, sucrose, and D-trehalose Rasamsonia emersonii NBRC 31232 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.9 ? x * 60000, SDS-PAGE Rasamsonia emersonii
1.1.5.9 ? x * 58000, SDS-PAGE Thermoascus crustaceus

Synonyms

EC Number Synonyms Comment Organism
1.1.5.9 FAD-dependent glucose dehydrogenase
-
 Rasamsonia emersonii
1.1.5.9 FAD-dependent glucose dehydrogenase
-
 Thermoascus crustaceus
1.1.5.9 FAD-GDH
-
 Rasamsonia emersonii
1.1.5.9 FAD-GDH
-
 Thermoascus crustaceus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.9 60 65
-
 Thermoascus crustaceus
1.1.5.9 65 70
-
 Rasamsonia emersonii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.5.9 40 70 after heat treatment at 40-70°C for 15 min, unglycosylated enzyme maintains nearly 100% activity at temperatures up to 45°C, but completely loses activity at 60°C, while glycosylated enzyme displays high thermostability, maintaining 89% activity at 60°C. The melting temperature of the glycosylated enzyme is 66.4°C Rasamsonia emersonii
1.1.5.9 40 62 unglycosylated enzym e is stable at temperatures up to 45°C , while glycosylatedenzyme maintains 75% activity at 60°C. The melting temperature of the glycosylated enzyme is 62.5°C Thermoascus crustaceus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.9 5
-
-
 Rasamsonia emersonii
1.1.5.9 5
-
-
 Thermoascus crustaceus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.5.9 2 9 the enzyme remains stable between pH 2.0 and 9.0 Rasamsonia emersonii
1.1.5.9 2 9 the enzyme remains stable between pH 2.0 and 9.0 Thermoascus crustaceus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.9 FAD
-
 Rasamsonia emersonii
1.1.5.9 FAD
-
 Thermoascus crustaceus