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Literature summary extracted from

  • Futai, M.
    Membrane D-lactate dehydrogenase from Escherichia coli. Purification and properties (1973), Biochemistry, 12, 2468-2474.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.5.12 cyanide stimulates D-lactate dehydrogenase activity at neutral pH, though it inhibits the activity at alkaline pH Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.5.12 cyanide stimulates D-lactate dehydrogenase activity at neutral pH, though it inhibits the activity at alkaline pH. At 1.5 mM, 50% inhibition at pH 9.3 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.12 0.6
-
 (R)-lactate pH 8.0, 23°C Escherichia coli
1.1.5.12 1.2
-
 (R,S)-2-hydroxybutanoate pH 8.0, 23°C Escherichia coli
1.1.5.12 1.4
-
 (R)-lactate presence of cyanide, pH 8.0, 23°C Escherichia coli
1.1.5.12 18
-
 (S)-lactate pH 8.0, 23°C Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.12 membrane
-
 Escherichia coli 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.12 71000
-
 sucrose density gradient centrifugation Escherichia coli
1.1.5.12 72000
-
 1 * 72000, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.12 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.12
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.1.5.12 -90°C, 20% ethylene glycol, stable for at least 2 weeks Escherichia coli
1.1.5.12 0°C, 5 days, 80% residual activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.12 (R)-lactate + phenazine methosulfate
-
 Escherichia coli pyruvate + reduced phenazine methosulfate
-
 ?
1.1.5.12 (R,S)-2-hydroxybutanoate + a quinone 12% of the activity with (R)-lactate Escherichia coli 2-oxobutanoate + a quinol
-
 ?
1.1.5.12 (S)-lactate + oxidized dichlorophenolindophenol 14% of the activity with (R)-lactate Escherichia coli pyruvate + reduced dichlorophenolindophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.12 monomer 1 * 72000, SDS-PAGE Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.12 8 9
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.12 FAD
-
 Escherichia coli