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Literature summary extracted from

  • Endo, S.; Noda, M.; Ikari, A.; Tatematsu, K.; El-Kabbani, O.; Hara, A.; Kitade, Y.; Matsunaga, T.
    Characterization of hamster NAD+-dependent 3(17)beta-hydroxysteroid dehydrogenase belonging to the aldo-keto reductase 1C subfamily (2015), J. Biochem., 158, 425-434.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.51 gene AKR1C35, cloned from liver, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS Mesocricetus auratus
1.1.1.218 gene AKR1C34, cloned from liver, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS Mesocricetus auratus
1.1.1.239 gene AKR1C35, cloned from liver, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS Mesocricetus auratus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.51 C310F site-directed mutagenesis, the mutation, in analogy to enzyme AKR1C34 sequence, does not affect the enzyme activity of AKR1C35 Mesocricetus auratus
1.1.1.51 V54A site-directed mutagenesis, the mutation, in analogy to enzyme AKR1C34 sequence, impairs the 3beta-hydroxysteroid dehydrogenase activity of AKR1C35 Mesocricetus auratus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.51 7-hydroxyflavone
-
 Mesocricetus auratus
1.1.1.51 chrysin
-
 Mesocricetus auratus
1.1.1.51 galangin
-
 Mesocricetus auratus
1.1.1.51 genistein
-
 Mesocricetus auratus
1.1.1.51 Hexestrol
-
 Mesocricetus auratus
1.1.1.51 hinokitiol
-
 Mesocricetus auratus
1.1.1.51 lithocholic acid
-
 Mesocricetus auratus
1.1.1.51 Phenolphthalein
-
 Mesocricetus auratus
1.1.1.51 quercetin
-
 Mesocricetus auratus
1.1.1.51 zearalenone
-
 Mesocricetus auratus
1.1.1.218 Hexestrol
-
 Mesocricetus auratus
1.1.1.218 hinokitiol
-
 Mesocricetus auratus
1.1.1.218 additional information no or poor inhibition by 7-hydroxyflavone, chrysin, quercetin, genistein, galangin, lithocholic acid, and zearalenone Mesocricetus auratus
1.1.1.218 Phenolphthalein
-
 Mesocricetus auratus
1.1.1.239 Hexestrol
-
 Mesocricetus auratus
1.1.1.239 hinokitiol
-
 Mesocricetus auratus
1.1.1.239 additional information no or poor inhibition by 7-hydroxyflavone, chrysin, quercetin, genistein, galangin, lithocholic acid, and zearalenone Mesocricetus auratus
1.1.1.239 Phenolphthalein
-
 Mesocricetus auratus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.51 additional information
-
 additional information Michaelis-Menten kinetics Mesocricetus auratus
1.1.1.51 0.0004
-
 17beta-estradiol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.0009
-
 3beta-hydroxyprogesterone pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.001
-
 geranylgeraniol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.001
-
 beta-ionol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.0012
-
 farnesol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.0012
-
 (S)-1-indanol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.0015
-
 (S)-1,2,3,4-tetrahydro-1-naphthol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.0025
-
 5alpha-Androstan-3beta-ol-17-one pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.0033
-
 5-androstene-3beta,17beta-diol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.0039
-
 5beta-androstane-3alpha,17beta-diol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.0039
-
 5alpha-pregnan-3beta-ol-20-one pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.0041
-
 geraniol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.0043
-
 dehydroepiandrosterone pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.0068
-
 5beta-Pregnan-3beta-ol-20-one pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.0076
-
 5alpha-dihydrotestosterone pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.0081
-
 testosterone pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.0081
-
 4-androstene-3alpha,17beta-diol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.0097
-
 cis-benzene dihydrodiol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.011
-
 nerol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.011
-
 trans-benzene dihydrodiol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.013
-
 5beta-dihydrotestosterone pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.014
-
 alpha-3-hydroxyhexobarbital pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.015
-
 5beta-androstane-3beta,17beta-diol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.015
-
 5beta-pregnan-3beta,21-diol-20-one pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.017
-
 NADH pH 7.4, 25°C, with substrate S-camphorquinone Mesocricetus auratus
1.1.1.51 0.023
-
 1-Acenaphthenol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.023
-
 NAD+ pH 7.4, 25°C, with substrate S-tetralol Mesocricetus auratus
1.1.1.51 0.026
-
 2-Cyclohexen-1-ol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.028
-
 (R)-1,2,3,4-tetrahydro-1-naphthol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.04
-
 beta-3-hydroxyhexobarbital pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.046
-
 5beta-androstan-3beta-ol-17-one pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.074
-
 Cyclohexanol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 2.4
-
 NADP+ pH 7.4, 25°C, with substrate S-camphorquinone Mesocricetus auratus
1.1.1.239 additional information
-
 additional information Michaelis-Menten kinetics Mesocricetus auratus
1.1.1.239 0.0012
-
 geranylgeraniol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.0097
-
 beta-ionol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.011
-
 farnesol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.013
-
 beta-3-hydroxyhexobarbital pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.013
-
 NADH pH 7.4, 25°C, with substrate S-camphorquinone Mesocricetus auratus
1.1.1.239 0.013
-
 NAD+ pH 7.4, 25°C, with substrate S-tetralol Mesocricetus auratus
1.1.1.239 0.03
-
 alpha-3-hydroxyhexobarbital pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.061
-
 (S)-1,2,3,4-tetrahydro-1-naphthol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.076
-
 geraniol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.095
-
 (R)-1,2,3,4-tetrahydro-1-naphthol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.148
-
 1-Acenaphthenol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.162
-
 nerol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.181
-
 (S)-1-indanol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.569
-
 2-Cyclohexen-1-ol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.63
-
 cis-benzene dihydrodiol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 1
-
 trans-benzene dihydrodiol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 1.38
-
 NADP+ pH 7.4, 25°C, with substrate S-camphorquinone Mesocricetus auratus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.51 cytosol
-
 Mesocricetus auratus 5829
-
1.1.1.239 cytosol
-
 Mesocricetus auratus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.51 37000
-
 x * 37000, recombinant His-tagged enzyme, SDS-PAGE Mesocricetus auratus
1.1.1.239 37000
-
 recombinant His-tagged enzyme, SDS-PAGE Mesocricetus auratus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.218 morphine + NAD+ Mesocricetus auratus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.51 Mesocricetus auratus A0A097ZMZ1
-
-
1.1.1.218 Mesocricetus auratus A0A097ZMY7
-
-
1.1.1.239 Mesocricetus auratus A0A097ZMY7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.51 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, ultrafiltration, and dialysis Mesocricetus auratus
1.1.1.218 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, ultrafiltration, and dialysis Mesocricetus auratus
1.1.1.239 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, ultrafiltration, and dialysis Mesocricetus auratus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.51 adrenal gland in female hamsters Mesocricetus auratus
-
1.1.1.51 brain in female hamsters Mesocricetus auratus
-
1.1.1.51 colon in female and male hamsters Mesocricetus auratus
-
1.1.1.51 heart in female hamsters Mesocricetus auratus
-
1.1.1.51 kidney in female hamsters Mesocricetus auratus
-
1.1.1.51 liver
-
 Mesocricetus auratus
-
1.1.1.51 liver in female and male hamsters Mesocricetus auratus
-
1.1.1.51 lung in female hamsters Mesocricetus auratus
-
1.1.1.51 additional information mRNA for AKR1C34 is expressed liver-specific in male hamsters and ubiquitously in female hamsters Mesocricetus auratus
-
1.1.1.51 ovary
-
 Mesocricetus auratus
-
1.1.1.51 small intestine in female hamsters Mesocricetus auratus
-
1.1.1.51 stomach in female hamsters Mesocricetus auratus
-
1.1.1.51 testis in female hamsters Mesocricetus auratus
-
1.1.1.218 adrenal gland in male hamsters Mesocricetus auratus
-
1.1.1.218 colon in male hamsters Mesocricetus auratus
-
1.1.1.218 heart in female hamsters Mesocricetus auratus
-
1.1.1.218 kidney in male hamsters Mesocricetus auratus
-
1.1.1.218 liver
-
 Mesocricetus auratus
-
1.1.1.218 additional information mRNA for AKR1C34 is expressed liver-specific in female hamsters and ubiquitously in male hamsters Mesocricetus auratus
-
1.1.1.218 small intestine in male hamsters Mesocricetus auratus
-
1.1.1.218 testis in male hamsters Mesocricetus auratus
-
1.1.1.239 adrenal gland in male hamsters Mesocricetus auratus
-
1.1.1.239 colon in male hamsters Mesocricetus auratus
-
1.1.1.239 heart in female hamsters Mesocricetus auratus
-
1.1.1.239 kidney in male hamsters Mesocricetus auratus
-
1.1.1.239 liver
-
 Mesocricetus auratus
-
1.1.1.239 additional information mRNA for AKR1C34 is expressed liver-specific in female hamsters and ubiquitously in male hamsters Mesocricetus auratus
-
1.1.1.239 small intestine in male hamsters Mesocricetus auratus
-
1.1.1.239 testis in male hamsters Mesocricetus auratus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.51 (R)-1,2,3,4-tetrahydro-1-naphthol + NAD+ i.e. R-tetralol Mesocricetus auratus 3,4-dihydronaphthalen-1(2H)-one + NADH + H+
-
 ?
1.1.1.51 (S)-1,2,3,4-tetrahydro-1-naphthol + NAD+ i.e. S-tetralol Mesocricetus auratus 3,4-dihydronaphthalen-1(2H)-one + NADH + H+
-
 ?
1.1.1.51 (S)-1,2,3,4-tetrahydro-1-naphthol + NADP+ i.e. S-tetralol Mesocricetus auratus 3,4-dihydronaphthalen-1(2H)-one + NADPH + H+
-
 ?
1.1.1.51 (S)-1-indanol + NAD+
-
 Mesocricetus auratus 2,3-dihydro-1H-inden-1-one + NADH + H+
-
 ?
1.1.1.51 (S)-camphorquinone + NADH + H+
-
 Mesocricetus auratus ? + NAD+
-
 ?
1.1.1.51 (S)-camphorquinone + NADPH + H+
-
 Mesocricetus auratus ? + NADP+
-
 ?
1.1.1.51 1-acenaphthenol + NAD+
-
 Mesocricetus auratus acenaphthylen-1(2H)-one + NADH + H+
-
 ?
1.1.1.51 17beta-estradiol + NAD+
-
 Mesocricetus auratus estrone + NADH + H+
-
 ?
1.1.1.51 2-cyclohexen-1-ol + NAD+
-
 Mesocricetus auratus 2-cyclohexen-1-one + NADH + H+
-
 ?
1.1.1.51 3beta-hydroxyprogesterone + NADH + H+
-
 Mesocricetus auratus ? + NAD+
-
 ?
1.1.1.51 4-androstene-3alpha,17beta-diol + NAD+
-
 Mesocricetus auratus ? + NADH + H+
-
 ?
1.1.1.51 5-androstene-3beta,17beta-diol + NAD+
-
 Mesocricetus auratus ? + NADH + H+
-
 ?
1.1.1.51 5alpha-androstan-3alpha-ol-17-one + NAD+
-
 Mesocricetus auratus 5alpha-androstan-3,17-dione + NADH + H+
-
 ?
1.1.1.51 5alpha-androstan-3beta-ol-17-one + NAD+
-
 Mesocricetus auratus 5alpha-androstan-3,17-dione + NADH + H+
-
 ?
1.1.1.51 5alpha-androstane-3alpha,17beta-diol + NAD+
-
 Mesocricetus auratus ?
-
 ?
1.1.1.51 5alpha-dihydrotestosterone + NADH + H+
-
 Mesocricetus auratus ? + NAD+
-
 ?
1.1.1.51 5alpha-pregnan-3alpha-ol-20-one + NAD+
-
 Mesocricetus auratus 5alpha-pregnan-3,20-dione + NADH + H+
-
 ?
1.1.1.51 5alpha-pregnan-3beta-ol-20-one + NAD+
-
 Mesocricetus auratus 5alpha-pregnan-3,20-dione + NADH + H+
-
 ?
1.1.1.51 5beta-androstan-17beta-ol-3-one + NAD+
-
 Mesocricetus auratus 5beta-androstan-3,17-dione + NADH + H+
-
 ?
1.1.1.51 5beta-androstan-3alpha-ol-17-one + NAD+
-
 Mesocricetus auratus 5beta-androstan-3,17-dione + NADH + H+
-
 ?
1.1.1.51 5beta-androstan-3beta-ol-17-one + NADH + H+
-
 Mesocricetus auratus ? + NAD+
-
 ?
1.1.1.51 5beta-androstane-3alpha,17beta-diol + NAD+
-
 Mesocricetus auratus ?
-
 ?
1.1.1.51 5beta-androstane-3beta,17beta-diol + NAD+
-
 Mesocricetus auratus ?
-
 ?
1.1.1.51 5beta-dihydrotestosterone + NADH + H+
-
 Mesocricetus auratus ? + NAD+
-
 ?
1.1.1.51 5beta-pregnan-20alpha-ol-3-one + NAD+
-
 Mesocricetus auratus 5beta-pregnan-3,20-dione + NADH + H+
-
 ?
1.1.1.51 5beta-pregnan-3beta,21-diol-20-one + NAD+
-
 Mesocricetus auratus ?
-
 ?
1.1.1.51 5beta-pregnan-3beta,21-diol-20-one + NADH + H+
-
 Mesocricetus auratus ? + NAD+
-
 ?
1.1.1.51 5beta-pregnan-3beta-ol-20-one + NAD+
-
 Mesocricetus auratus 5beta-pregnan-3,20-dione + NADH + H+
-
 ?
1.1.1.51 alpha-3-hydroxyhexobarbital + NAD+
-
 Mesocricetus auratus ?
-
 ?
1.1.1.51 beta-3-hydroxyhexobarbital + NAD+
-
 Mesocricetus auratus ?
-
 ?
1.1.1.51 beta-ionol + NAD+
-
 Mesocricetus auratus (3E)-4-(2,6,6-trimethylcyclohex-1-en-1-yl)but-3-en-2-one + NADH + H+
-
 ?
1.1.1.51 cis-benzene dihydrodiol + NAD+
-
 Mesocricetus auratus ?
-
 ?
1.1.1.51 cyclohexanol + NAD+
-
 Mesocricetus auratus ?
-
 ?
1.1.1.51 dehydroepiandrosterone + NADH + H+
-
 Mesocricetus auratus ? + NAD+
-
 ?
1.1.1.51 farnesol + NAD+
-
 Mesocricetus auratus farnesal + NADH + H+
-
 ?
1.1.1.51 geraniol + NAD+
-
 Mesocricetus auratus geranial + NADH + H+
-
 ?
1.1.1.51 geranylgeraniol + NAD+
-
 Mesocricetus auratus geranylgeranial + NADH + H+
-
 ?
1.1.1.51 additional information the enzyme AKR1C35 efficiently oxidizes various 3beta- and 17beta-hydroxysteroids, including biologically active 3beta-hydroxy-5alpha/beta-dihydro-C19/C21-steroids, dehydroepiandrosterone, and 17beta-estradiol, substrate specificity, detailed overview. Val54 plays a critical role in non-/recognition of the steroidal substrates. Reaction products identification by thin-layer chromatography (TLC) and liquid chromatography/mass spectrometry (LC/MS). No activity with 5beta-pregnan-3alpha-ol-20-one, 3alpha-hydroxyprogesterone, 5beta-androstan-3alpha-ol-17-one, 20alpha-hydroxyprogesterone, and 5alpha-pregnan-20alpha-ol-3-one Mesocricetus auratus ?
-
 ?
1.1.1.51 nerol + NAD+
-
 Mesocricetus auratus neral + NADH + H+
-
 ?
1.1.1.51 testosterone + NAD+
-
 Mesocricetus auratus 4-androstenedione + NADH + H+
-
 ?
1.1.1.51 trans-benzene dihydrodiol + NAD+
-
 Mesocricetus auratus ?
-
 ?
1.1.1.218 additional information the enzyme AKR1C34 shows 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase activities, EC 1.1.1.239, detailed overview. Ala54 plays a critical role in non-/recognition of the steroidal substrates. Reaction products identification by thin-layer chromatography (TLC) and liquid chromatography/mass spectrometry (LC/MS) Mesocricetus auratus ?
-
 ?
1.1.1.218 morphine + NAD+
-
 Mesocricetus auratus ?
-
 ?
1.1.1.218 trans-naphthalene dihydrodiol + NAD+
-
 Mesocricetus auratus ?
-
 ?
1.1.1.239 (R)-1,2,3,4-tetrahydro-1-naphthol + NAD+ i.e. (R)-tetralol Mesocricetus auratus 3,4-dihydronaphthalen-1(2H)-one + NADH + H+
-
 ?
1.1.1.239 (S)-1,2,3,4-tetrahydro-1-naphthol + NAD+ i.e. (S)-tetralol Mesocricetus auratus 3,4-dihydronaphthalen-1(2H)-one + NADH + H+
-
 ?
1.1.1.239 (S)-1,2,3,4-tetrahydro-1-naphthol + NADP+ i.e. (S)-tetralol Mesocricetus auratus 3,4-dihydronaphthalen-1(2H)-one + NADPH + H+
-
 ?
1.1.1.239 (S)-1-indanol + NAD+
-
 Mesocricetus auratus 2,3-dihydro-1H-inden-1-one + NADH + H+
-
 ?
1.1.1.239 (S)-camphorquinone + NADH + H+
-
 Mesocricetus auratus ? + NAD+
-
 ?
1.1.1.239 (S)-camphorquinone + NADPH + H+
-
 Mesocricetus auratus ? + NADP+
-
 ?
1.1.1.239 1-acenaphthenol + NAD+
-
 Mesocricetus auratus acenaphthylen-1(2H)-one + NADH + H+
-
 ?
1.1.1.239 17beta-estradiol + NAD+
-
 Mesocricetus auratus estrone + NADH + H+
-
 ?
1.1.1.239 2-cyclohexen-1-ol + NAD+
-
 Mesocricetus auratus 2-cyclohexen-1-one + NADH + H+
-
 ?
1.1.1.239 20alpha-hydroxyprogesterone + NADH + H+
-
 Mesocricetus auratus ? + NAD+
-
 ?
1.1.1.239 3alpha-hydroxyprogesterone + NADH + H+
-
 Mesocricetus auratus ? + NAD+
-
 ?
1.1.1.239 3beta-hydroxyprogesterone + NADH + H+
-
 Mesocricetus auratus ? + NAD+
-
 ?
1.1.1.239 5alpha-androstan-3alpha-ol-17-one + NAD+
-
 Mesocricetus auratus 5alpha-androstan-3,17-dione + NADH + H+
-
 ?
1.1.1.239 5alpha-androstan-3beta-ol-17-one + NAD+
-
 Mesocricetus auratus 5alpha-androstan-3,17-dione + NADH + H+
-
 ?
1.1.1.239 5alpha-androstane-3alpha,17beta-diol + NAD+
-
 Mesocricetus auratus ?
-
 ?
1.1.1.239 5alpha-androstane-3beta,17beta-diol + NAD+
-
 Mesocricetus auratus ?
-
 ?
1.1.1.239 5alpha-dihydrotestosterone + NAD+
-
 Mesocricetus auratus 5alpha-androstane-3,17-dione + NADH + H+
-
 ?
1.1.1.239 5alpha-pregnan-20alpha-ol-3-one + NAD+
-
 Mesocricetus auratus 5alpha-pregnan-3,20-dione + NADH + H+
-
 ?
1.1.1.239 5alpha-pregnan-3alpha-ol-20-one + NAD+
-
 Mesocricetus auratus 5alpha-pregnan-3,20-dione + NADH + H+
-
 ?
1.1.1.239 5alpha-pregnan-3beta-ol-20-one + NAD+
-
 Mesocricetus auratus 5alpha-pregnan-3,20-dione + NADH + H+
-
 ?
1.1.1.239 5beta-androstan-17beta-ol-3-one + NAD+
-
 Mesocricetus auratus 5beta-androstan-3,17-dione + NADH + H+
-
 ?
1.1.1.239 5beta-androstan-3alpha-ol-17-one + NAD+
-
 Mesocricetus auratus 5beta-androstan-3,17-dione + NADH + H+
-
 ?
1.1.1.239 5beta-androstane-3alpha,17beta-diol + NAD+
-
 Mesocricetus auratus ?
-
 ?
1.1.1.239 5beta-androstane-3beta,17beta-diol + NAD+
-
 Mesocricetus auratus ?
-
 ?
1.1.1.239 5beta-dihydrotestosterone + NAD+
-
 Mesocricetus auratus androstanedione + NADH + H+
-
 ?
1.1.1.239 5beta-pregnan-20alpha-ol-3-one + NAD+
-
 Mesocricetus auratus 5beta-pregnan-3,20-dione + NADH + H+
-
 ?
1.1.1.239 5beta-pregnan-3alpha-ol-20-one + NAD+
-
 Mesocricetus auratus 5beta-pregnan-3,20-dione + NADH + H+
-
 ?
1.1.1.239 5beta-pregnan-3beta,21-diol-20-one + NAD+
-
 Mesocricetus auratus ?
-
 ?
1.1.1.239 5beta-pregnan-3beta-ol-20-one + NAD+
-
 Mesocricetus auratus 5beta-pregnan-3,20-dione + NADH + H+
-
 ?
1.1.1.239 alpha-3-hydroxyhexobarbital + NAD+
-
 Mesocricetus auratus ?
-
 ?
1.1.1.239 beta-3-hydroxyhexobarbital + NAD+
-
 Mesocricetus auratus ?
-
 ?
1.1.1.239 beta-ionol + NAD+
-
 Mesocricetus auratus (3E)-4-(2,6,6-trimethylcyclohex-1-en-1-yl)but-3-en-2-one + NADH + H+
-
 ?
1.1.1.239 cis-benzene dihydrodiol + NAD+
-
 Mesocricetus auratus ?
-
 ?
1.1.1.239 dehydroepiandrosterone + NAD+
-
 Mesocricetus auratus ? + NADH + H+
-
 ?
1.1.1.239 farnesol + NAD+
-
 Mesocricetus auratus farnesal + NADH + H+
-
 ?
1.1.1.239 geraniol + NAD+
-
 Mesocricetus auratus geranial + NADH + H+
-
 ?
1.1.1.239 geranylgeraniol + NAD+
-
 Mesocricetus auratus geranylgeranial + NADH + H+
-
 ?
1.1.1.239 additional information the enzyme AKR1C34 shows 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase activities, substrate specificity, detailed overview. Ala54 plays a critical role in non-/recognition of the steroidal substrates. Reaction products identification by thin-layer chromatography (TLC) and liquid chromatography/mass spectrometry (LC/MS). AKR1C34 also has morphine dehydrogenase activity, EC 1.1.1.218. No activity with cyclohexanol Mesocricetus auratus ?
-
 ?
1.1.1.239 nerol + NAD+
-
 Mesocricetus auratus neral + NADH + H+
-
 ?
1.1.1.239 testosterone + NAD+
-
 Mesocricetus auratus 4-androstenedione + NADH + H+
-
 ?
1.1.1.239 trans-benzene dihydrodiol + NAD+
-
 Mesocricetus auratus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.51 ? x * 37000, recombinant His-tagged enzyme, SDS-PAGE Mesocricetus auratus
1.1.1.239 ? x * 37000, recombinant His-tagged enzyme, SDS-PAGE Mesocricetus auratus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.51 AKR1C35
-
 Mesocricetus auratus
1.1.1.51 LOC101842245
-
 Mesocricetus auratus
1.1.1.218 AKR1C34
-
 Mesocricetus auratus
1.1.1.218 morphine-6-dehydrogenase UniProt Mesocricetus auratus
1.1.1.239 AKR1C34
-
 Mesocricetus auratus
1.1.1.239 More cf. EC 1.1.1.218, morphine 6-dehydrogenase Mesocricetus auratus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.51 25
-
 assay at Mesocricetus auratus
1.1.1.218 25
-
 assay at Mesocricetus auratus
1.1.1.239 25
-
 assay at Mesocricetus auratus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.51 0.0028
-
 5beta-Pregnan-3beta-ol-20-one pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.0043
-
 5beta-pregnan-3beta,21-diol-20-one pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.0078
-
 testosterone pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.008
-
 geranylgeraniol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.009
-
 Cyclohexanol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.01
-
 dehydroepiandrosterone pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.012
-
 5beta-androstane-3alpha,17beta-diol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.013
-
 5alpha-pregnan-3beta-ol-20-one pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.018
-
 farnesol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.018
-
 alpha-3-hydroxyhexobarbital pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.022
-
 17beta-estradiol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.022
-
 1-Acenaphthenol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.022
-
 5beta-androstan-3beta-ol-17-one pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.022
-
 beta-ionol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.022
-
 (R)-1,2,3,4-tetrahydro-1-naphthol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.023
-
 geraniol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.023
-
 nerol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.025
-
 cis-benzene dihydrodiol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.025
-
 (S)-1,2,3,4-tetrahydro-1-naphthol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.025
-
 beta-3-hydroxyhexobarbital pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.027
-
 5alpha-Androstan-3beta-ol-17-one pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.028
-
 (S)-1-indanol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.032
-
 5beta-dihydrotestosterone pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.033
-
 trans-benzene dihydrodiol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.036
-
 5beta-androstane-3beta,17beta-diol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.04
-
 2-Cyclohexen-1-ol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.043
-
 5-androstene-3beta,17beta-diol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.043
-
 4-androstene-3alpha,17beta-diol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.056
-
 3beta-hydroxyprogesterone pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.057
-
 5alpha-dihydrotestosterone pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.1
-
 NADH pH 7.4, 25°C, with substrate S-camphorquinone Mesocricetus auratus
1.1.1.51 0.11
-
 NAD+ pH 7.4, 25°C, with substrate S-tetralol Mesocricetus auratus
1.1.1.51 0.15
-
 NADP+ pH 7.4, 25°C, with substrate S-camphorquinone Mesocricetus auratus
1.1.1.239 0.0045
-
 farnesol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.0075
-
 (R)-1,2,3,4-tetrahydro-1-naphthol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.0153
-
 geraniol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.025
-
 NAD+ pH 7.4, 25°C, with substrate S-tetralol Mesocricetus auratus
1.1.1.239 0.028
-
 2-Cyclohexen-1-ol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.032
-
 cis-benzene dihydrodiol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.038
-
 geranylgeraniol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.04
-
 alpha-3-hydroxyhexobarbital pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.045
-
 trans-benzene dihydrodiol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.053
-
 beta-ionol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.0917
-
 NADP+ pH 7.4, 25°C, with substrate S-camphorquinone Mesocricetus auratus
1.1.1.239 0.0967
-
 beta-3-hydroxyhexobarbital pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.1
-
 (S)-1-indanol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.105
-
 (S)-1,2,3,4-tetrahydro-1-naphthol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.115
-
 nerol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.167
-
 NADH pH 7.4, 25°C, with substrate S-camphorquinone Mesocricetus auratus
1.1.1.239 0.283
-
 1-Acenaphthenol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.51 9
-
 substrate oxidation Mesocricetus auratus
1.1.1.218 7.4
-
 assay at Mesocricetus auratus
1.1.1.239 10
-
 substrate oxidation Mesocricetus auratus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.51 additional information the enzyme shows high preference for NAD+ compared with NADP+ Mesocricetus auratus
1.1.1.51 NAD+ conserved residues Gln270 and Glu276 are critical residues for the NAD+ specificity Mesocricetus auratus
1.1.1.51 NADH
-
 Mesocricetus auratus
1.1.1.51 NADP+
-
 Mesocricetus auratus
1.1.1.51 NADPH
-
 Mesocricetus auratus
1.1.1.239 additional information the enzyme shows high preference for NAD+ compared with NADP+ Mesocricetus auratus
1.1.1.239 NAD+ conserved residues Gln270 and Glu276 are critical residues for the NAD+ specificity Mesocricetus auratus
1.1.1.239 NADH
-
 Mesocricetus auratus
1.1.1.239 NADP+
-
 Mesocricetus auratus
1.1.1.239 NADPH
-
 Mesocricetus auratus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.51 0.00011
-
 chrysin pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.00011
-
 7-hydroxyflavone pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.00039
-
 lithocholic acid pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.00046
-
 quercetin pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.00054
-
 Hexestrol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.00055
-
 genistein pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.00069
-
 galangin pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.0033
-
 zearalenone pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.51 0.00092
-
 pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus 7-hydroxyflavone
1.1.1.51 0.001
-
 pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus chrysin
1.1.1.51 0.0026
-
 pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus quercetin
1.1.1.51 0.0042
-
 pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus genistein
1.1.1.51 0.0044
-
 pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus lithocholic acid
1.1.1.51 0.005
-
 pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus galangin
1.1.1.51 0.0065
-
 pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus Hexestrol
1.1.1.51 0.015
-
 pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus zearalenone
1.1.1.51 0.05
-
 above, pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus Phenolphthalein
1.1.1.51 0.05
-
 above, pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus hinokitiol
1.1.1.218 0.00065
-
 pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus Phenolphthalein
1.1.1.218 0.0013
-
 pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus Hexestrol
1.1.1.218 0.0032
-
 pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus hinokitiol
1.1.1.239 0.00065
-
 pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus Phenolphthalein
1.1.1.239 0.0032
-
 pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus hinokitiol
1.1.1.239 0.013
-
 pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus Hexestrol

General Information

EC Number General Information Comment Organism
1.1.1.51 evolution enzyme AKR1C35 is a member of the aldo–keto reductase (AKR) 1C subfamily in the AKR superfamily Mesocricetus auratus
1.1.1.51 malfunction mutation of Val54Ala, but not Cys310Phe, significantly impairs the 3beta-hydroxysteroid dehydrogenase enzyme activity, suggesting that Val54 plays a critical role in recognition of the steroidal substrate Mesocricetus auratus
1.1.1.51 physiological function enzyme AKR1C35 oxidizes various xenobiotic alicyclic alcohols using NAD+ Mesocricetus auratus
1.1.1.218 evolution enzyme M6DH is a member of the aldo–keto reductase (AKR) 1C subfamily in the AKR superfamily Mesocricetus auratus
1.1.1.239 evolution enzyme AKR1C34 is a member of the aldo–keto reductase (AKR) 1C subfamily in the AKR superfamily Mesocricetus auratus
1.1.1.239 physiological function enzyme AKR1C34 oxidizes various xenobiotic alicyclic alcohols using NAD+ Mesocricetus auratus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.51 0.063
-
 NADP+ pH 7.4, 25°C, with substrate S-camphorquinone Mesocricetus auratus
1.1.1.51 0.117
-
 Cyclohexanol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.28
-
 5beta-pregnan-3beta,21-diol-20-one pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.42
-
 5beta-Pregnan-3beta-ol-20-one pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.47
-
 5beta-androstan-3beta-ol-17-one pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.6
-
 5beta-dihydrotestosterone pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.63
-
 beta-3-hydroxyhexobarbital pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.78
-
 (R)-1,2,3,4-tetrahydro-1-naphthol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 0.95
-
 1-Acenaphthenol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 1.3
-
 alpha-3-hydroxyhexobarbital pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 1.55
-
 2-Cyclohexen-1-ol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 2.17
-
 nerol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 2.33
-
 dehydroepiandrosterone pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 2.5
-
 cis-benzene dihydrodiol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 2.5
-
 5beta-androstane-3beta,17beta-diol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 2.83
-
 5alpha-dihydrotestosterone pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 3
-
 trans-benzene dihydrodiol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 3
-
 5beta-androstane-3alpha,17beta-diol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 3.33
-
 5alpha-pregnan-3beta-ol-20-one pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 3.5
-
 testosterone pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 5
-
 NAD+ pH 7.4, 25°C, with substrate S-tetralol Mesocricetus auratus
1.1.1.51 5.5
-
 4-androstene-3alpha,17beta-diol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 5.67
-
 geraniol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 6
-
 NADH pH 7.4, 25°C, with substrate S-camphorquinone Mesocricetus auratus
1.1.1.51 7.67
-
 geranylgeraniol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 9.67
-
 5-androstene-3beta,17beta-diol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 10.67
-
 5alpha-Androstan-3beta-ol-17-one pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 14.17
-
 farnesol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 16.67
-
 (S)-1,2,3,4-tetrahydro-1-naphthol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 21.67
-
 beta-ionol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 23.3
-
 (S)-1-indanol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 63.33
-
 3beta-hydroxyprogesterone pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.51 116.7
-
 17beta-estradiol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.05
-
 trans-benzene dihydrodiol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.05
-
 2-Cyclohexen-1-ol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.05
-
 cis-benzene dihydrodiol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.067
-
 nerol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.067
-
 NADP+ pH 7.4, 25°C, with substrate S-camphorquinone Mesocricetus auratus
1.1.1.239 0.083
-
 (R)-1,2,3,4-tetrahydro-1-naphthol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.2
-
 geraniol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.42
-
 farnesol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 0.55
-
 (S)-1-indanol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 1.67
-
 (S)-1,2,3,4-tetrahydro-1-naphthol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 1.83
-
 1-Acenaphthenol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 2
-
 NAD+ pH 7.4, 25°C, with substrate S-tetralol Mesocricetus auratus
1.1.1.239 2.17
-
 alpha-3-hydroxyhexobarbital pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 5.3
-
 beta-ionol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 7.5
-
 beta-3-hydroxyhexobarbital pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus
1.1.1.239 12.67
-
 NADH pH 7.4, 25°C, with substrate S-camphorquinone Mesocricetus auratus
1.1.1.239 33.3
-
 geranylgeraniol pH 7.4, 25°C, recombinant enzyme Mesocricetus auratus