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Literature summary extracted from

  • Becker-Kettern, J.; Paczia, N.; Conrotte, J.F.; Kay, D.P.; Guignard, C.; Jung, P.P.; Linster, C.L.
    Saccharomyces cerevisiae forms D-2-hydroxyglutarate and couples its degradation to D-lactate formation via a cytosolic transhydrogenase (2016), J. Biol. Chem., 291, 6036-6058.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.40 0.111
-
D-2-hydroxyglutarate pH 9.5, 30°C Saccharomyces cerevisiae
1.1.99.40 0.45
-
pyruvate pH 9.5, 30°C Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.40 cytosol
-
Saccharomyces cerevisiae 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.99.40 Co2+ 0.005 mM, about 3fold stimulation Saccharomyces cerevisiae
1.1.99.40 Zn2+ 0.005 mM, about 3fold stimulation Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.40 Saccharomyces cerevisiae P39976
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.40 recombinant protein. Purified fractions show a yellow color Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.40 2-oxoglutarate + D-lactate
-
Saccharomyces cerevisiae D-2-hydroxyglutarate + pyruvate
-
r
1.1.99.40 D-2-hydroxyglutarate + oxaloacetate
-
Saccharomyces cerevisiae 2-oxoglutarate + malate
-
r
1.1.99.40 D-2-hydroxyglutarate + pyruvate
-
Saccharomyces cerevisiae 2-oxoglutarate + D-lactate
-
r
1.1.99.40 additional information no substrate: L-lactate. The enzyme displays dehydrogenase activity with artificial acceptor dichlorophenolindophenol Saccharomyces cerevisiae ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.99.40 D-2-hydroxyglutarate-pyruvate transhydrogenase
-
Saccharomyces cerevisiae
1.1.99.40 DLD3
-
Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.40 4
-
D-2-hydroxyglutarate pH 9.5, 30°C Saccharomyces cerevisiae
1.1.99.40 4.9
-
pyruvate pH 9.5, 30°C Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.40 FAD enzyme is a FAD-dependent transhydrogenase using pyruvate as a hydrogen acceptor Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
1.1.99.40 physiological function a mutant strain lacking cytosolic transhydrogenase Dld3 activity accumulates millimolar levels of D-2-hydroxyglutarate Saccharomyces cerevisiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.99.40 12
-
pyruvate pH 9.5, 30°C Saccharomyces cerevisiae
1.1.99.40 36
-
D-2-hydroxyglutarate pH 9.5, 30°C Saccharomyces cerevisiae