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Literature summary extracted from

  • Peek, J.; Shi, T.; Christendat, D.
    Identification of novel polyphenolic inhibitors of shikimate dehydrogenase (AroE) (2014), J. Biomol. Screen., 19, 1090-1098.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.25 gene aroE, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 CodonPlus Arabidopsis thaliana
1.1.1.25 gene aroE, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 CodonPlus Pseudomonas putida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.25 crystal structure analysis, PDB ID 2O7S Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.25 2,2-bisepigallocatechin gallate about 50% inhibition at 0.0025 mM Pseudomonas putida
1.1.1.25 aurintricarboxylic acid lower inhibitry potency, about 25% inhibition at 0.0025 mM Pseudomonas putida
1.1.1.25 baicalein about 25% inhibition at 0.0025 mM Pseudomonas putida
1.1.1.25 cardiolipin lower inhibitry potency, about 25% inhibition at 0.0025 mM Pseudomonas putida
1.1.1.25 dianthrol about 50% inhibition at 0.0025 mM Pseudomonas putida
1.1.1.25 diethylenetriamine pentaacetic acid lower inhibitry potency, about 25% inhibition at 0.0025 mM Pseudomonas putida
1.1.1.25 ebselen lower inhibitry potency, about 25% inhibition at 0.0025 mM Pseudomonas putida
1.1.1.25 ellagic acid about 50% inhibition at 0.0025 mM Pseudomonas putida
1.1.1.25 epicatechin gallate inhibits the AroE domain of the bifunctional dehydroquinate dehydratase-shikimate dehydrogenase (DHQ-SDH) from Arabidopsis thaliana Arabidopsis thaliana
1.1.1.25 epicatechin gallate over 75% inhibition at 0.0025 mM Pseudomonas putida
1.1.1.25 epigallocatechin gallate inhibits the AroE domain of the bifunctional dehydroquinate dehydratase-shikimate dehydrogenase (DHQ-SDH) from Arabidopsis thaliana Arabidopsis thaliana
1.1.1.25 epigallocatechin gallate over 75% inhibition at 0.0025 mM Pseudomonas putida
1.1.1.25 epigallocatechin-3,5-digallate about 50% inhibition at 0.0025 mM Pseudomonas putida
1.1.1.25 epitheaflavin monogallate about 50% inhibition at 0.0025 mM Pseudomonas putida
1.1.1.25 hydroquinone lower inhibitry potency, about 25% inhibition at 0.0025 mM Pseudomonas putida
1.1.1.25 merbromin lower inhibitry potency, about 25% inhibition at 0.0025 mM Pseudomonas putida
1.1.1.25 additional information screening for polyphenolc enzyme inhibitors, overview Arabidopsis thaliana
1.1.1.25 additional information screening for polyphenolc enzyme inhibitors, overview. No inhibition by gallic acid, epigallocatechin and epicatechin Pseudomonas putida
1.1.1.25 nordihydroguaiaretic acid lower inhibitry potency, about 25% inhibition at 0.0025 mM Pseudomonas putida
1.1.1.25 purpurogallin about 50% inhibition at 0.0025 mM Pseudomonas putida
1.1.1.25 pyridoxine lower inhibitry potency, about 25% inhibition at 0.0025 mM Pseudomonas putida
1.1.1.25 pyrogallin about 50% inhibition at 0.0025 mM Pseudomonas putida
1.1.1.25 quercetin about 25% inhibition at 0.0025 mM Pseudomonas putida
1.1.1.25 taxifolin about 25% inhibition at 0.0025 mM Pseudomonas putida
1.1.1.25 theaflavanin about 25% inhibition at 0.0025 mM Pseudomonas putida
1.1.1.25 theaflavin monogallate about 50% inhibition at 0.0025 mM Pseudomonas putida
1.1.1.25 theaflavin-3,3-digallate about 25% inhibition at 0.0025 mM Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.25 3-dehydroshikimate + NADPH Arabidopsis thaliana
-
shikimate + NADP+
-
r
1.1.1.25 shikimate + NADP+ Pseudomonas putida
-
3-dehydroshikimate + NADPH + H+
-
r
1.1.1.25 shikimate + NADP+ Pseudomonas putida KT 2240
-
3-dehydroshikimate + NADPH + H+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.25 Arabidopsis thaliana Q9SQT8
-
-
1.1.1.25 Pseudomonas putida Q88RQ5
-
-
1.1.1.25 Pseudomonas putida KT 2240 Q88RQ5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.25 recombinant His6-tagged enzyme from Escherichia coli strain BL21 CodonPlus by nickel affinity chromatography and dialysis Arabidopsis thaliana
1.1.1.25 recombinant His6-tagged enzyme from Escherichia coli strain BL21 CodonPlus by nickel affinity chromatography and dialysis Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.25 3-dehydroshikimate + NADPH
-
Arabidopsis thaliana shikimate + NADP+
-
r
1.1.1.25 additional information The bifunctional enzyme also catalyzes dehydration of 3-dehydroquinate to 3-dehydroshikimate Arabidopsis thaliana ?
-
?
1.1.1.25 shikimate + NADP+
-
Pseudomonas putida 3-dehydroshikimate + NADPH + H+
-
r
1.1.1.25 shikimate + NADP+
-
Pseudomonas putida KT 2240 3-dehydroshikimate + NADPH + H+
-
r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.25 AroE
-
Arabidopsis thaliana
1.1.1.25 AroE
-
Pseudomonas putida
1.1.1.25 bifunctional dehydroquinate dehydratase-shikimate dehydrogenase
-
Arabidopsis thaliana
1.1.1.25 DHQ-SDH
-
Arabidopsis thaliana
1.1.1.25 SDH
-
Arabidopsis thaliana
1.1.1.25 SDH
-
Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.25 25
-
assay at Arabidopsis thaliana
1.1.1.25 25
-
assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.25 8.8
-
assay at Arabidopsis thaliana
1.1.1.25 8.8
-
assay at Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.25 NADP+
-
Arabidopsis thaliana
1.1.1.25 NADP+
-
Pseudomonas putida
1.1.1.25 NADPH
-
Arabidopsis thaliana
1.1.1.25 NADPH
-
Pseudomonas putida

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.25 0.002
-
pH 8.8, 25°C, recombinant enzyme, AroE domain of the DQD-SDH enzyme Arabidopsis thaliana epicatechin gallate
1.1.1.25 0.0021
-
pH 8.8, 25°C, recombinant enzyme, AroE domain of the DQD-SDH enzyme Arabidopsis thaliana epigallocatechin gallate
1.1.1.25 0.003
-
pH 8.8, 25°C, recombinant enzyme Pseudomonas putida epigallocatechin gallate
1.1.1.25 0.0037
-
pH 8.8, 25°C, recombinant enzyme Pseudomonas putida epicatechin gallate