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Literature summary extracted from
- Boost in bioethanol production using recombinant Saccharomyces cerevisiae with mutated strictly NADPH-dependent xylose reductase and NADP+-dependent xylitol dehydrogenase (2013), J. Biotechnol., 165, 153-156.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.10 | recombinant overexpression of strictly NADPH-dependent XR with mutated strictly NADP+-dependent XDH leading to increased bioethanol production and decreased xylitol accumulation in Saccharomyces cerevisiae strain D452-2 resulting in mutant strains SK-N1 to SK-N4 | Scheffersomyces stipitis |
| 1.1.1.431 | recombinant overexpression of strictly NADPH-dependent xylose reductase with mutated strictly NADP+-dependent xylitol dehydrogenase leading to increased bioethanol production and decreased xylitol accumulation in Saccharomyces cerevisiae strain D452-2 resulting in mutant strains SK-N1 to SK-N4 | Scheffersomyces stipitis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.1.1.9 | additional information | enzyme XDH is changed from NAD+-dependent to NADP+-dependent, xylitol accumulation is reduced and ethanol production improved using protein engineering for reversing the dependency of XDH from NAD+ to NADP+. Construction of a set of recombinant Saccharomyces cerevisiae carrying a mutated strictly NADPH-dependent XR and NADP+-dependent XDH genes with overexpression of endogenous xylulokinase (XK), effects of complete NADPH/NADP+ recycling on ethanol fermentation and xylitol accumulation, overview. The mutated strains demonstrate 0% and 10% improvement in ethanol production, and reduced xylitol accumulation, ranging 34.4-54.7% compared with the control strain | Scheffersomyces stipitis |
| 1.1.1.10 | additional information | enzyme XDH is changed from NAD+-dependent to NADP+-dependent, xylitol accumulation is reduced and ethanol production improved using protein engineering for reversing the dependency of XDH from NAD+ to NADP+. Construction of a set of recombinant Saccharomyces cerevisiae carrying a mutated strictly NADPH-dependent XR and NADP+-dependent XDH genes with overexpression of endogenous xylulokinase (XK), effects of complete NADPH/NADP+ recycling on ethanol fermentation and xylitol accumulation, overview. The mutated strains demonstrate 0% and 10% improvement in ethanol production, and reduced xylitol accumulation, ranging 34.4-54.7% compared with the control strain | Scheffersomyces stipitis |
| 1.1.1.431 | additional information | construction of a set of recombinant Saccharomyces cerevisiae carrying a mutated strictly NADPH-dependent xylose reductase and NADP+-dependent xylitol dehydrogenase genes with overexpression of endogenous xylulokinase (XK), effects of complete NADPH/NADP+ recycling on ethanol fermentation and xylitol accumulation, overview. The mutated strains demonstrate 0% and 10% improvement in ethanol production, and reduced xylitol accumulation, ranging 34.4-54.7% compared with the control strain | Scheffersomyces stipitis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.431 | xylitol + NAD+ | Scheffersomyces stipitis | - |
D-xylose + NADH + H+ | - |
r |  |
| 1.1.1.431 | xylitol + NADP+ | Scheffersomyces stipitis | - |
D-xylose + NADPH + H+ | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.9 | Scheffersomyces stipitis | P22144 | i.e Pichia stipitis | - |
| 1.1.1.10 | Scheffersomyces stipitis | - |
i.e Pichia stipitis | - |
| 1.1.1.431 | Scheffersomyces stipitis | P31867 | i.e Pichia stipitis | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.10 | 14.11 | 15.29 | recombinant strains harboring genes Xr and mutated XDH, pH and temperature not specified in the publication | Scheffersomyces stipitis |
| 1.1.1.431 | 1.11 | 2.52 | recombinant strains harboring genes xyl1 and mutated XDH, pH and temperature not specified in the publication, substrates D-xylosse and NADPH | Scheffersomyces stipitis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.9 | xylitol + NAD+ | - |
Scheffersomyces stipitis | L-xylulose + NADH + H+ | - |
? | |
| 1.1.1.10 | xylitol + NADP+ | engineered enzyme with altered cofactor specificity | Scheffersomyces stipitis | L-xylulose + NADPH + H+ | - |
? | |
| 1.1.1.431 | xylitol + NAD+ | - |
Scheffersomyces stipitis | D-xylose + NADH + H+ | - |
r | |
| 1.1.1.431 | xylitol + NADP+ | - |
Scheffersomyces stipitis | D-xylose + NADPH + H+ | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.9 | NAD+-dependent xylitol dehydrogenase | - |
Scheffersomyces stipitis |
| 1.1.1.9 | XDH | - |
Scheffersomyces stipitis |
| 1.1.1.10 | NADP+-dependent xylitol dehydrogenase | - |
Scheffersomyces stipitis |
| 1.1.1.10 | XDH | - |
Scheffersomyces stipitis |
| 1.1.1.431 | NAD(P)H-dependent D-xylose reductase | - |
Scheffersomyces stipitis |
| 1.1.1.431 | NADPH-dependent xylose reductase | - |
Scheffersomyces stipitis |
| 1.1.1.431 | XYL1 | - |
Scheffersomyces stipitis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.9 | NAD+ | specific for | Scheffersomyces stipitis | |
| 1.1.1.10 | NADP+ | engineered enzyme with altered cofactor specificity | Scheffersomyces stipitis | |
| 1.1.1.431 | NAD+ | - |
Scheffersomyces stipitis | |
| 1.1.1.431 | NADH | - |
Scheffersomyces stipitis | |
| 1.1.1.431 | NADP+ | - |
Scheffersomyces stipitis | |
| 1.1.1.431 | NADPH | - |
Scheffersomyces stipitis | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.1.1.9 | metabolism | the redox balance between xylose reductase (XR) and xylitol dehydrogenase (XDH) is thought to be an important factor in effective xylose fermentation | Scheffersomyces stipitis |
| 1.1.1.9 | physiological function | strictly NADPH-dependent XR with mutated strict NADP+-dependent XDH are more effective in increasing bioethanol production and decreasing xylitol accumulation than the NAD+-dependent wild-type XDH, overview | Scheffersomyces stipitis |
| 1.1.1.10 | metabolism | the redox balance between xylose reductase (XR) and xylitol dehydrogenase (XDH) is thought to be an important factor in effective xylose fermentation | Scheffersomyces stipitis |
| 1.1.1.10 | physiological function | strictly NADPH-dependent XR with mutated strict NADP+-dependent XDH are more effective in increasing bioethanol production and decreasing xylitol accumulation than the wild-type, overview | Scheffersomyces stipitis |
| 1.1.1.431 | metabolism | the redox balance between xylose reductase (XR) and xylitol dehydrogenase (XDH, EC 1.1.1.10) is thought to be an important factor in effective xylose fermentation | Scheffersomyces stipitis |
| 1.1.1.431 | physiological function | strictly NADPH-dependent xylose reductase with mutated strict NADP+-dependent xylitol dehydrogenase, EC 1.1.1.10, are more effective in increasing bioethanol production and decreasing xylitol accumulation than the wild-type, overview | Scheffersomyces stipitis |
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