Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Park, S.A.; Park, Y.S.; Lee, K.S.
    Kinetic characterization and molecular modeling of NAD(P)(+)-dependent succinic semialdehyde dehydrogenase from Bacillus subtilis as an ortholog YneI (2014), J. Microbiol. Biotechnol., 24, 954-958.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.16 expression in Escherichia coli Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.16 Succinic semialdehyde complete uncompetitive substrate inhibition Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.16 0.21
-
 NAD+ pH not specified in the publication, 30°C Bacillus subtilis
1.2.1.16 0.39
-
 NADP+ pH not specified in the publication, 30°C Bacillus subtilis
1.2.1.16 10.36
-
 succinate semialdehyde cofactor NAD+, pH not specified in the publication, 30°C Bacillus subtilis
1.2.1.16 20.5
-
 succinate semialdehyde cofactor NADP+, pH not specified in the publication, 30°C Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.16 Bacillus subtilis P94428
-
-
1.2.1.16 Bacillus subtilis 168 P94428
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.16 succinate semialdehyde + NAD+ + H2O
-
 Bacillus subtilis succinate + NADH + 2 H+
-
 ?
1.2.1.16 succinate semialdehyde + NAD+ + H2O
-
 Bacillus subtilis 168 succinate + NADH + 2 H+
-
 ?
1.2.1.16 succinate semialdehyde + NADP+ + H2O
-
 Bacillus subtilis succinate + NADPH + 2 H+
-
 ?
1.2.1.16 succinate semialdehyde + NADP+ + H2O
-
 Bacillus subtilis 168 succinate + NADPH + 2 H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.16 SSADH
-
 Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.16 5.26
-
 NAD+ pH not specified in the publication, 30°C Bacillus subtilis
1.2.1.16 5.61
-
 succinate semialdehyde cofactor NAD+, pH not specified in the publication, 30°C Bacillus subtilis
1.2.1.16 7.24
-
 succinate semialdehyde cofactor NADP+, pH not specified in the publication, 30°C Bacillus subtilis
1.2.1.16 9.93
-
 NADP+ pH not specified in the publication, 30°C Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.16 NAD+ similar values of the catalytic efficiency (kcat/Km) with both NAD+ and NADP+ as cofactors Bacillus subtilis
1.2.1.16 NADP+ similar values of the catalytic efficiency (kcat/Km) with both NAD+ and NADP+ as cofactors Bacillus subtilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.16 0.36
-
 succinate semialdehyde cofactor NADP+, pH not specified in the publication, 30°C Bacillus subtilis
1.2.1.16 0.6
-
 succinate semialdehyde cofactor NAD+, pH not specified in the publication, 30°C Bacillus subtilis
1.2.1.16 25.1
-
 NAD+ pH not specified in the publication, 30°C Bacillus subtilis
1.2.1.16 25.4
-
 NADP+ pH not specified in the publication, 30°C Bacillus subtilis