EC Number | Cloned (Comment) | Organism |
---|---|---|
5.5.1.B7 | expressed in Nicotiana benthamiana and Escherichia coli BL21DE3-C41 cells | Salvia divinorum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.5.1.B7 | F255A/N313A | the mutation directs product specificity completely towards formation of ent-8alpha/betalambda-13E-en-8,15-diol | Salvia divinorum |
5.5.1.B7 | F255H | the mutation alters the product outcome from (-)-kolavenol to ent-copalol as the sole product | Salvia divinorum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.5.1.B7 | Salvia divinorum | A0A1L3THX5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.5.1.B7 | Ni2+-affinity column chromatography and Superdex 200 gel filtration | Salvia divinorum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
5.5.1.B7 | trichome | specific expression in trichomes | Salvia divinorum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.5.1.B7 | geranylgeranyl diphosphate | - |
Salvia divinorum | (-)-kolavenol | - |
? | |
5.5.1.B7 | geranylgeranyl diphosphate | the reaction proceeds through conversion of the precursor geranylgeranyl diphosphate into the clerodienyl diphosphate intermediate via a series of 1,2-hydride and methyl shifts of the intermediary labda-13-en-8-yl diphosphate intermediate | Salvia divinorum | clerodienyl diphosphate + H+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.5.1.B7 | clerodienyl diphosphate synthase | - |
Salvia divinorum |
5.5.1.B7 | CLPP synthase | - |
Salvia divinorum |
5.5.1.B7 | CPS2 | - |
Salvia divinorum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
5.5.1.B7 | metabolism | the enzyme catalyzes the committed step in salvinorin A biosynthesis | Salvia divinorum |