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Literature summary extracted from

  • Larsson, K.A.; Saheed, S.A.; Gradin, T.; Delp, G.; Karpinska, B.; Botha, C.E.; Jonsson, L.M.
    Differential regulation of 3-aminomethylindole/N-methyl-3-aminomethylindole N-methyltransferase and gramine in barley by both biotic and abiotic stress conditions (2011), Plant Physiol. Biochem., 49, 96-102.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.340 additional information gramine levels, but not NMT expression, is increased in barley due to drought and abscisic acid treatment Hordeum vulgare

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.340 expression in Escherichia coli Hordeum vulgare subsp. vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.340 0.077
-
 3-aminomethylindole pH 7.5, 30°C Hordeum vulgare subsp. vulgare
2.1.1.340 0.148
-
 S-adenosyl-L-methionine pH 7.5, 30°C Hordeum vulgare subsp. vulgare
2.1.1.340 0.184
-
 N-methyl-3-aminomethylindole pH 7.5, 30°C Hordeum vulgare subsp. vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.340 43000
-
 x * 43000, SDS-PAGE Hordeum vulgare subsp. vulgare

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.340 Hordeum vulgare
-
-
-
2.1.1.340 Hordeum vulgare subsp. vulgare Q96565
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.340 2 S-adenosyl-L-methionine + 3-aminomethylindole
-
 Hordeum vulgare subsp. vulgare 2 S-adenosyl-L-homocysteine + gramine overall reaction, two successive N-methylation reactions ?
2.1.1.340 2 S-adenosyl-L-methionine + N-methyl-3-aminomethylindole
-
 Hordeum vulgare subsp. vulgare 2 S-adenosyl-L-homocysteine + (1H-indol-3-yl)-N,N-dimethylmethanamine
-
 ?
2.1.1.340 additional information no substrate: caffeic acid Hordeum vulgare subsp. vulgare ?
-
 ?
2.1.1.340 S-adenosyl-L-methionine + 3-aminomethylindole
-
 Hordeum vulgare subsp. vulgare S-adenosyl-L-homocysteine + N-methyl-3-aminomethylindole
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.340 ? x * 43000, SDS-PAGE Hordeum vulgare subsp. vulgare

Synonyms

EC Number Synonyms Comment Organism
2.1.1.340 NMT
-
 Hordeum vulgare

Expression

EC Number Organism Comment Expression
2.1.1.340 Hordeum vulgare expression is induced by methyl jasmonate. Infestation by Rhopalosiphum padi and Metopolophium dirhodum results in higher expression in the doubled haploid line 5172-28:4. None of the aphide-plant combinations displays increased gramine levels, and the increased abundance of transcript in aphid-infested DH28:4 does not lead to higher amounts of NMT protein or NMT enzyme activity up