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Literature summary extracted from

  • Gudmundsson, M.; Hansson, H.; Karkehabadi, S.; Larsson, A.; Stals, I.; Kim, S.; Sunux, S.; Fujdala, M.; Larenas, E.; Kaper, T.; Sandgren, M.
    Structural and functional studies of the glycoside hydrolase family 3 beta-glucosidase Cel3A from the moderately thermophilic fungus Rasamsonia emersonii (2016), Acta Crystallogr. Sect. D, 72, 860-870 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.21 degradation enzyme shows increased thermal stability and saccharification yield on pretreated corn stover compared with Hypocrea jecorina Cel3A Rasamsonia emersonii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 expression in Hypocrea jecorina Rasamsonia emersonii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.21 to 0.98 A resolution. Enzyme is a highly N-glycosylated three-domain dimeric protein Rasamsonia emersonii
3.2.1.21 to 2.2 A resolution. Enzyme is a highly N-glycosylated three-domain dimeric protein Rasamsonia emersonii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.39
-
 cellotriose pH 5.0, 37°C Rasamsonia emersonii
3.2.1.21 0.4
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 37°C Rasamsonia emersonii
3.2.1.21 0.4
-
 2-chloro-4-nitrophenyl-beta-D-glucopyranoside pH 5.0, 37°C Rasamsonia emersonii
3.2.1.21 0.4
-
 2-chloro-4-nitrophenyl beta-D-glucopyranoside pH 5.0, 37°C Rasamsonia emersonii
3.2.1.21 0.78
-
 cellobiose pH 5.0, 37°C Rasamsonia emersonii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Rasamsonia emersonii Q8TGI8
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.21 glycoprotein protein is highly N-glycosylated Rasamsonia emersonii
3.2.1.21 glycoprotein crystallization data Rasamsonia emersonii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 2-chloro-4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Rasamsonia emersonii 2-chloro-4-nitrophenol + D-glucose
-
 ?
3.2.1.21 2-chloro-4-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Rasamsonia emersonii 2-chloro-4-nitrophenol + D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Rasamsonia emersonii 4-nitrophenol + D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Rasamsonia emersonii 2 D-glucose
-
 ?
3.2.1.21 cellotriose + 2 H2O
-
 Rasamsonia emersonii 3 D-glucose
-
 ?
3.2.1.21 additional information enzyme shows a preference for disaccharide substrates over longer glucooligosaccharides Rasamsonia emersonii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 dimer crystallization data Rasamsonia emersonii
3.2.1.21 dimer gel filtration and crystallization data Rasamsonia emersonii

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 Cel3A
-
 Rasamsonia emersonii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 87.3
-
 melting temperature Rasamsonia emersonii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 0.72
-
 cellotriose pH 5.0, 37°C Rasamsonia emersonii
3.2.1.21 5.4
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 37°C Rasamsonia emersonii
3.2.1.21 5.5
-
 cellobiose pH 5.0, 37°C Rasamsonia emersonii
3.2.1.21 14
-
 2-chloro-4-nitrophenyl-beta-D-glucopyranoside pH 5.0, 37°C Rasamsonia emersonii
3.2.1.21 14
-
 2-chloro-4-nitrophenyl beta-D-glucopyranoside pH 5.0, 37°C Rasamsonia emersonii
3.2.1.21 55
-
 cellobiose pH 5.0, 37°C Rasamsonia emersonii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.21 1.9
-
 cellotriose pH 5.0, 37°C Rasamsonia emersonii
3.2.1.21 7.1
-
 cellobiose pH 5.0, 37°C Rasamsonia emersonii
3.2.1.21 14
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 37°C Rasamsonia emersonii
3.2.1.21 36
-
 2-chloro-4-nitrophenyl-beta-D-glucopyranoside pH 5.0, 37°C Rasamsonia emersonii
3.2.1.21 36
-
 2-chloro-4-nitrophenyl beta-D-glucopyranoside pH 5.0, 37°C Rasamsonia emersonii