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Literature summary extracted from
- Purification, characterization and application of lipoxygenase isoenzymes from Lasiodiplodia theobromae (2015), Appl. Biochem. Biotechnol., 175, 513-525 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.13.11.12 | food industry | addition of LOX1 and LOX2 altered the elasticity as well as viscosity of dough prepared from bleached wheat flour, LOX improves the dough rheology | Lasiodiplodia theobromae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.12 | additional information | enzyme LOX1 is not inhibited by jasmonic acid; enzyme LOX2 is not inhibited by jasmonic acid | Lasiodiplodia theobromae |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.12 | additional information | - |
additional information | kinetics | Lasiodiplodia theobromae |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.12 | 45000 | - |
gel filtration | Lasiodiplodia theobromae |
| 1.13.11.12 | 93000 | - |
gel filtration | Lasiodiplodia theobromae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.12 | linoleate + O2 | Lasiodiplodia theobromae | - |
9-hydroperoxy octadecadienoic acid + 13-hydroperoxy octadecadienoic acid | 9-hydroperoxy octadecadienoic acid is the product of EC 1.13.11.58 | ? |  |
| 1.13.11.12 | linoleate + O2 | Lasiodiplodia theobromae MTCC 3068 | - |
9-hydroperoxy octadecadienoic acid + 13-hydroperoxy octadecadienoic acid | 9-hydroperoxy octadecadienoic acid is the product of EC 1.13.11.58 | ? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.12 | Lasiodiplodia theobromae | - |
- |
- |
| 1.13.11.12 | Lasiodiplodia theobromae MTCC 3068 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.11.12 | native enzyme 203.5fold by gel filtration and anion exchange chromatography | Lasiodiplodia theobromae |
| 1.13.11.12 | native enzyme 578fold by gel filtration and anion exchange chromatography | Lasiodiplodia theobromae |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.13.11.12 | mycelium | - |
Lasiodiplodia theobromae | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.12 | 5538 | - |
purified native enzyme, pH 6.0, 40°C | Lasiodiplodia theobromae |
| 1.13.11.12 | 15733.3 | - |
purified native enzyme, pH 6.0, 40°C | Lasiodiplodia theobromae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.12 | linoleate + O2 | - |
Lasiodiplodia theobromae | 9-hydroperoxy octadecadienoic acid + 13-hydroperoxy octadecadienoic acid | 9-hydroperoxy octadecadienoic acid is the product of EC 1.13.11.58 | ? | |
| 1.13.11.12 | linoleate + O2 | - |
Lasiodiplodia theobromae MTCC 3068 | 9-hydroperoxy octadecadienoic acid + 13-hydroperoxy octadecadienoic acid | 9-hydroperoxy octadecadienoic acid is the product of EC 1.13.11.58 | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.13.11.12 | monomer | 1 * 45000, SDS-PAGE | Lasiodiplodia theobromae |
| 1.13.11.12 | monomer | 1 * 93000, SDS-PAGE | Lasiodiplodia theobromae |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.13.11.12 | LOX1 | - |
Lasiodiplodia theobromae |
| 1.13.11.12 | LOX2 | - |
Lasiodiplodia theobromae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.12 | 40 | - |
- |
Lasiodiplodia theobromae |
| 1.13.11.12 | 50 | - |
- |
Lasiodiplodia theobromae |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.12 | 30 | 75 | activity range | Lasiodiplodia theobromae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.12 | 6 | - |
- |
Lasiodiplodia theobromae |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.13.11.12 | physiological function | the lipoxygenase oxidizes linoleic acid into hydroperoxy octadecadienoic acid (HPOD), which is important in food and flavour industries for production of bread and flavouring compounds | Lasiodiplodia theobromae |
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