EC Number | Application | Comment | Organism |
---|---|---|---|
1.10.3.2 | degradation | laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment. The enzyme alone can decolourize indigo carmine partially after 60-min incubation at 45 °C. Decolorization is much more efficient in the presence of syringaldehyde. Nearly 90 % decolorization is observed within 20 min | Streptomyces griseorubens |
1.10.3.2 | environmental protection | laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment. The enzyme alone can decolorize indigo carmine partially after 60-min incubation at 45°C. Decolorization is much more efficient in the presence of syringaldehyde. Nearly 90 % decolorization is observed within 20 min | Streptomyces griseorubens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.10.3.2 | expression in Escherichia coli | Streptomyces griseorubens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.10.3.2 | CdCl2 | 1 mM, 24.6% inhibition | Streptomyces griseorubens | |
1.10.3.2 | CoCl2 | 1 mM, 20.8% inhibition | Streptomyces griseorubens | |
1.10.3.2 | dithiothreitol | 1 mM, 10% loss of activity | Streptomyces griseorubens | |
1.10.3.2 | EDTA | 1 mM, 62% loss of activity | Streptomyces griseorubens | |
1.10.3.2 | HgCl2 | 1 mM, 33.3% inhibition | Streptomyces griseorubens | |
1.10.3.2 | NaN3 | 1 mM, 5.5% loss of activity | Streptomyces griseorubens | |
1.10.3.2 | SDS | 1 mM, 20% loss of activity | Streptomyces griseorubens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.10.3.2 | extracellular | - |
Streptomyces griseorubens | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.10.3.2 | CuCl2 | 1 mM, increases activity by 28.5% | Streptomyces griseorubens | |
1.10.3.2 | MnCl2 | 1 mM, increases activity by 11.7% | Streptomyces griseorubens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.10.3.2 | Streptomyces griseorubens | - |
- |
- |
1.10.3.2 | Streptomyces griseorubens JSD-1 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.10.3.2 | 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Streptomyces griseorubens | ? | - |
? | |
1.10.3.2 | 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Streptomyces griseorubens JSD-1 | ? | - |
? | |
1.10.3.2 | 2,6-dimethoxyphenol + O2 | - |
Streptomyces griseorubens | ? | - |
? | |
1.10.3.2 | 2,6-dimethoxyphenol + O2 | - |
Streptomyces griseorubens JSD-1 | ? | - |
? | |
1.10.3.2 | guaiacol + O2 | - |
Streptomyces griseorubens | ? | - |
? | |
1.10.3.2 | guaiacol + O2 | - |
Streptomyces griseorubens JSD-1 | ? | - |
? | |
1.10.3.2 | syringaldazine + O2 | - |
Streptomyces griseorubens | ? | - |
? | |
1.10.3.2 | syringaldazine + O2 | - |
Streptomyces griseorubens JSD-1 | ? | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 30 | - |
8 h, 40% loss of activity | Streptomyces griseorubens |
1.10.3.2 | 40 | - |
8 h, 60% loss of activity | Streptomyces griseorubens |
1.10.3.2 | 50 | - |
8 h, 70% loss of activity | Streptomyces griseorubens |
1.10.3.2 | 60 | - |
8 h, 90% loss of activity | Streptomyces griseorubens |
1.10.3.2 | 70 | - |
8 h, complete loss of activity | Streptomyces griseorubens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.10.3.2 | 0.058 | - |
2,6-dimethoxyphenol | 25°C, pH 9.0 | Streptomyces griseorubens | |
1.10.3.2 | 0.337 | - |
syringaldazine | 25°C, pH 8.0 | Streptomyces griseorubens | |
1.10.3.2 | 7.7 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | 25°C, pH 4.0 | Streptomyces griseorubens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 4 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Streptomyces griseorubens |
1.10.3.2 | 8 | - |
substrate: syringaldazine | Streptomyces griseorubens |
1.10.3.2 | 9 | - |
substrate: 2,6-dimethoxyphenol | Streptomyces griseorubens |
1.10.3.2 | 10 | - |
substrate: guaiacol | Streptomyces griseorubens |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 3 | - |
complete loss of activity within 1 day | Streptomyces griseorubens |
1.10.3.2 | 4 | 5 | enzyme loses 50% activity within 2 days | Streptomyces griseorubens |
1.10.3.2 | 7 | 11 | the enzyme retains over 50% residual activity after 5-day incubation | Streptomyces griseorubens |
1.10.3.2 | 10 | - |
highest stability is observed at pH 10.0 with an approximately residual activity of 90% after 5 days | Streptomyces griseorubens |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.10.3.2 | Streptomyces griseorubens | expression levels were are upregulated with the stimulation of rice straw or lignin. However, it iss expressed in relative low levels when induced by cellulose, xylan, pectin or glucose | up |