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Literature summary extracted from

  • Puntus, I.; Vlasova, E.; Sokolov, A.; Zakharchenko, N.; Funtikova, T.
    Properties of non-homologous salicylate hydroxylases of Pseudomonas bacteria (2015), Appl. Biochem. Microbiol., 51, 215-221 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.1 additional information not activated by FMN and riboflavin Pseudomonas fluorescens
1.14.13.1 additional information not activated by FMN and riboflavin Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.1 0.0013
-
 salicylate at pH 7.5, temperature not specified in the publication Pseudomonas fluorescens
1.14.13.1 0.0031
-
 salicylate at pH 7.5, temperature not specified in the publication Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.1 salicylate + NADH + H+ + O2 Pseudomonas fluorescens
-
 catechol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 salicylate + NADH + H+ + O2 Pseudomonas putida
-
 catechol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 salicylate + NADH + H+ + O2 Pseudomonas fluorescens 142 NF
-
 catechol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 salicylate + NADH + H+ + O2 Pseudomonas putida BS3701
-
 catechol + NAD+ + H2O + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.1 Pseudomonas fluorescens
-
-
-
1.14.13.1 Pseudomonas fluorescens 142 NF
-
-
-
1.14.13.1 Pseudomonas putida
-
-
-
1.14.13.1 Pseudomonas putida BS3701
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.1 DEAE-Toyopearl 650M column chromatography, phenyl Sepharose CL6B column chromatography, and Sephacryl gel filtration Pseudomonas fluorescens
1.14.13.1 DEAE-Toyopearl 650M column chromatography, phenyl Sepharose CL6B column chromatography, and Sephacryl gel filtration Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.1 0.63
-
 after 45fold purification, at pH 7.5, temperature not specified in the publication Pseudomonas putida
1.14.13.1 7.55
-
 after 69fold purification, at pH 7.5, temperature not specified in the publication Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.1 1-hydroxy-2-naphthoate + NADH + H+ + O2 25% activity compared to salicylate Pseudomonas fluorescens ?
-
 ?
1.14.13.1 1-hydroxy-2-naphthoate + NADH + H+ + O2 83% activity compared to salicylate Pseudomonas putida ?
-
 ?
1.14.13.1 1-hydroxy-2-naphthoate + NADH + H+ + O2 25% activity compared to salicylate Pseudomonas fluorescens 142 NF ?
-
 ?
1.14.13.1 1-hydroxy-2-naphthoate + NADH + H+ + O2 83% activity compared to salicylate Pseudomonas putida BS3701 ?
-
 ?
1.14.13.1 3-aminosalicylate + NADH + H+ + O2 11% activity compared to salicylate Pseudomonas fluorescens 3-aminobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 3-aminosalicylate + NADH + H+ + O2 62% activity compared to salicylate Pseudomonas putida 3-aminobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 3-aminosalicylate + NADH + H+ + O2 11% activity compared to salicylate Pseudomonas fluorescens 142 NF 3-aminobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 3-aminosalicylate + NADH + H+ + O2 62% activity compared to salicylate Pseudomonas putida BS3701 3-aminobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 3-chlorosalicylate + NADH + H+ + O2 15% activity compared to salicylate Pseudomonas fluorescens 3-chlorobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 3-chlorosalicylate + NADH + H+ + O2 23% activity compared to salicylate Pseudomonas putida 3-chlorobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 3-chlorosalicylate + NADH + H+ + O2 15% activity compared to salicylate Pseudomonas fluorescens 142 NF 3-chlorobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 3-chlorosalicylate + NADH + H+ + O2 23% activity compared to salicylate Pseudomonas putida BS3701 3-chlorobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 3-methylsalicylate + NADH + H+ + O2 47% activity compared to salicylate Pseudomonas fluorescens 3-methylbenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 3-methylsalicylate + NADH + H+ + O2 86% activity compared to salicylate Pseudomonas putida 3-methylbenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 4-aminosalicylate + NADH + H+ + O2 130% activity compared to salicylate Pseudomonas putida 4-aminobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 4-aminosalicylate + NADH + H+ + O2 94% activity compared to salicylate Pseudomonas fluorescens 4-aminobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 4-chlorosalicylate + NADH + H+ + O2 43% activity compared to salicylate Pseudomonas fluorescens 4-chlorobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 4-chlorosalicylate + NADH + H+ + O2 78% activity compared to salicylate Pseudomonas putida 4-chlorobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 4-methylsalicylate + NADH + H+ + O2 164% activity compared to salicylate Pseudomonas putida 4-methylbenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 4-methylsalicylate + NADH + H+ + O2 166% activity compared to salicylate Pseudomonas fluorescens 4-methylbenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 5-aminosalicylate + NADH + H+ + O2 50% activity compared to salicylate Pseudomonas putida 5-aminobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 5-aminosalicylate + NADH + H+ + O2 80% activity compared to salicylate Pseudomonas fluorescens 5-aminobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 5-chlorosalicylate + NADH + H+ + O2 14% activity compared to salicylate Pseudomonas fluorescens 5-chlorobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 5-chlorosalicylate + NADH + H+ + O2 18% activity compared to salicylate Pseudomonas putida 5-chlorobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 5-hydroxysalicylate + NADH + H+ + O2 132% activity compared to salicylate Pseudomonas fluorescens 2,5-dihydroxyphenol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 5-hydroxysalicylate + NADH + H+ + O2 84% activity compared to salicylate Pseudomonas putida 2,5-dihydroxyphenol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 5-methylsalicylate + NADH + H+ + O2 40% activity compared to salicylate Pseudomonas putida 4-methylbenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 5-methylsalicylate + NADH + H+ + O2 81% activity compared to salicylate Pseudomonas fluorescens 4-methylbenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 salicylate + NADH + H+ + O2
-
 Pseudomonas fluorescens catechol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 salicylate + NADH + H+ + O2
-
 Pseudomonas putida catechol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 salicylate + NADH + H+ + O2 100% activity Pseudomonas fluorescens catechol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 salicylate + NADH + H+ + O2 100% activity Pseudomonas putida catechol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 salicylate + NADH + H+ + O2
-
 Pseudomonas fluorescens 142 NF catechol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 salicylate + NADH + H+ + O2 100% activity Pseudomonas fluorescens 142 NF catechol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 salicylate + NADH + H+ + O2
-
 Pseudomonas putida BS3701 catechol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 salicylate + NADH + H+ + O2 100% activity Pseudomonas putida BS3701 catechol + NAD+ + H2O + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.1 monomer 1 * 46000, SDS-PAGE Pseudomonas fluorescens
1.14.13.1 monomer 1 * 46000, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.14.13.1 NahG
-
 Pseudomonas fluorescens
1.14.13.1 NahG
-
 Pseudomonas putida
1.14.13.1 NahU
-
 Pseudomonas fluorescens
1.14.13.1 NahU
-
 Pseudomonas putida
1.14.13.1 salicylate hydroxylase
-
 Pseudomonas fluorescens
1.14.13.1 salicylate hydroxylase
-
 Pseudomonas putida
1.14.13.1 salicylate, NADH: oxygen oxidoreductase
-
 Pseudomonas fluorescens
1.14.13.1 salicylate, NADH: oxygen oxidoreductase
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.1 FAD
-
 Pseudomonas fluorescens
1.14.13.1 FAD
-
 Pseudomonas putida
1.14.13.1 NADH
-
 Pseudomonas fluorescens
1.14.13.1 NADH
-
 Pseudomonas putida