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Literature summary extracted from

  • Banthiya, S.; Pekarova, M.; Kuhn, H.; Heydeck, D.
    Secreted lipoxygenase from Pseudomonas aeruginosa exhibits biomembrane oxygenase activity and induces hemolysis in human red blood cells (2015), Arch. Biochem. Biophys., 584, 116-124 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.12 recombinant overexpression of His-tagged enzyme in Escherichia coli BL21(DE3) Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.12 extracellular the enzyme is secreted Pseudomonas aeruginosa
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.12 arachidonic acid + O2 Pseudomonas aeruginosa
-
 (5Z,8Z,11Z, 13E,15S)-15-hydroperoxy-5,8,11,13-eicosatetraenoic acid
-
 ?
1.13.11.12 linoleic acid + O2 Pseudomonas aeruginosa
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate
-
 ?
1.13.11.12 additional information Pseudomonas aeruginosa mitochondrial membrane oxygenase activity of the enzyme PA-LOX, overview. PA-LOX is capable of oxygenating both, arachidonic acid and linoleic acid, the two major polyenoic fatty acids found in animal biomembranes. The enzyme binds phospholipids at its active site ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.12 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.12 recombinant His-tagged enzyme from Escherichia coli BL21(DE3)by nickel affinity chromatography and Pseudomonas aeruginosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.12 arachidonic acid + O2
-
 Pseudomonas aeruginosa (5Z,8Z,11Z, 13E,15S)-15-hydroperoxy-5,8,11,13-eicosatetraenoic acid
-
 ?
1.13.11.12 linoleic acid + O2
-
 Pseudomonas aeruginosa (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate
-
 ?
1.13.11.12 additional information mitochondrial membrane oxygenase activity of the enzyme PA-LOX, overview. PA-LOX is capable of oxygenating both, arachidonic acid and linoleic acid, the two major polyenoic fatty acids found in animal biomembranes. The enzyme binds phospholipids at its active site Pseudomonas aeruginosa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.12 LOX
-
 Pseudomonas aeruginosa
1.13.11.12 PA-LOX
-
 Pseudomonas aeruginosa
1.13.11.12 secreted lipoxygenase
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.12 additional information
-
 additional information kcat of the enzyme with mitochondrial membranes as substrate is 0.3/s Pseudomonas aeruginosa
1.13.11.12 162.4
-
 linoleic acid pH and temperature not specified in the publication Pseudomonas aeruginosa

General Information

EC Number General Information Comment Organism
1.13.11.12 physiological function secreted lipoxygenase from Pseudomonas aeruginosa exhibits biomembrane oxygenase activity and induces hemolysis in human red blood cells. Secreted lipoxygenase oxygenates free arachidonic acid predominantly to 15S-hydro(peroxy)-5Z,8Z,11Z,13E-eicosatetraenoic acid. The enzyme is capable of binding phospholipids at its active site and physically interacts with model membranes Pseudomonas aeruginosa