Any feedback?
Literature summary extracted from
- Biochemical and spectroscopic characterization of the non-heme Fe(II)- and 2-oxoglutarate-dependent ethylene-forming enzyme from Pseudomonas syringae pv. phaseolicola PK2 (2016), Biochemistry, 55, 5989-5999 .
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.12.19 | ascorbate | required | Pseudomonas savastanoi pv. phaseolicola |  |
| 1.14.20.7 | ascorbate | required | Pseudomonas savastanoi pv. phaseolicola | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.13.12.19 | gene efe, large scale expression of His6-tagged enzyme in Escherichia coli strain BL21 Gold (DE3), method optimization and evaluation | Pseudomonas savastanoi pv. phaseolicola |
| 1.14.20.7 | gene efe, large scale expression of His6-tagged enzyme in Escherichia coli strain BL21 Gold (DE3), method optimization and evaluation | Pseudomonas savastanoi pv. phaseolicola |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.20.7 | 0.031 | - |
2-oxoadipate | pH 7.5, 25°C, recombinant enzyme | Pseudomonas savastanoi pv. phaseolicola | |
| 1.14.20.7 | 0.05 | - |
L-arginine | with 2-oxoglutarate as cosubstrate, at pH 7.5 and 25°C | Pseudomonas savastanoi pv. phaseolicola | |
| 1.14.20.7 | 0.057 | - |
2-oxoglutarate | pH 7.5, 25°C, recombinant enzyme | Pseudomonas savastanoi pv. phaseolicola | |
| 1.14.20.7 | 0.071 | - |
L-arginine | with 2-oxoadipate as cosubstrate, at pH 7.5 and 25°C | Pseudomonas savastanoi pv. phaseolicola | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.12.19 | Fe2+ | dependent on, required for catalysis | Pseudomonas savastanoi pv. phaseolicola | |
| 1.14.20.7 | Fe2+ | dependent on, required for catalysis | Pseudomonas savastanoi pv. phaseolicola | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.19 | 39370 | - |
recombinant detagged enzyme, gel filtration | Pseudomonas savastanoi pv. phaseolicola |
| 1.14.20.7 | 39370 | - |
recombinant detagged enzyme, gel filtration | Pseudomonas savastanoi pv. phaseolicola |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.12.19 | 2-oxoglutarate + O2 | Pseudomonas savastanoi pv. phaseolicola | - |
ethylene + 3 CO2 + H2O | - |
? | |
| 1.13.12.19 | 2-oxoglutarate + O2 | Pseudomonas savastanoi pv. phaseolicola PK2 | - |
ethylene + 3 CO2 + H2O | - |
? | |
| 1.14.20.7 | 2-oxoglutarate + L-arginine + O2 | Pseudomonas savastanoi pv. phaseolicola | - |
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O | - |
? | |
| 1.14.20.7 | 2-oxoglutarate + L-arginine + O2 | Pseudomonas savastanoi pv. phaseolicola PK2 | - |
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.12.19 | Pseudomonas savastanoi pv. phaseolicola | P32021 | - |
- |
| 1.13.12.19 | Pseudomonas savastanoi pv. phaseolicola PK2 | P32021 | - |
- |
| 1.14.20.7 | Pseudomonas savastanoi pv. phaseolicola | P32021 | i.e. Kudzu strain | - |
| 1.14.20.7 | Pseudomonas savastanoi pv. phaseolicola PK2 | P32021 | i.e. Kudzu strain | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.12.19 | recombinant His-tagged enzyme from Escherichia coli strain BL21 Gold (DE3) by nickel affinity chromatography, tag cleavage by a TEV protease mutant, and another step of nickel affinity chromatography, and dialysis of the flow through | Pseudomonas savastanoi pv. phaseolicola |
| 1.14.20.7 | recombinant His-tagged enzyme from Escherichia coli strain BL21 Gold (DE3) by nickel affinity chromatography, tag cleavage by a TEV protease mutant, and another step of nickel affinity chromatography, and dialysis of the flow through | Pseudomonas savastanoi pv. phaseolicola |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.14.20.7 | L-arginine + 2-oxoglutarate + O2 = succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O | reaction mechanism | Pseudomonas savastanoi pv. phaseolicola |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.12.19 | 2-oxoglutarate + O2 | - |
Pseudomonas savastanoi pv. phaseolicola | ethylene + 3 CO2 + H2O | - |
? | |
| 1.13.12.19 | 2-oxoglutarate + O2 | - |
Pseudomonas savastanoi pv. phaseolicola PK2 | ethylene + 3 CO2 + H2O | - |
? | |
| 1.13.12.19 | additional information | cf. EC 1.14.11.34, reaction via 5-hydroxyarginine. Selected L-Arg derivatives induce ethylene formation without undergoing hydroxylation, demonstrating that ethylene production and L-Arg hydroxylation activities are not linked. Enzyme EFE utilizes the alternative 2-oxo acid 2-oxoadipate as a cosubstrate (forming glutaric acid) during the hydroxylation of L-Arg, with this reaction unlinked from ethylene formation. The amount of ethylene produced is more than twice the levels of succinate, L-DELTA1-pyrroline-5-carboxylate, or guanidine generated | Pseudomonas savastanoi pv. phaseolicola | ? | - |
? | |
| 1.13.12.19 | additional information | cf. EC 1.14.11.34, reaction via 5-hydroxyarginine. Selected L-Arg derivatives induce ethylene formation without undergoing hydroxylation, demonstrating that ethylene production and L-Arg hydroxylation activities are not linked. Enzyme EFE utilizes the alternative 2-oxo acid 2-oxoadipate as a cosubstrate (forming glutaric acid) during the hydroxylation of L-Arg, with this reaction unlinked from ethylene formation. The amount of ethylene produced is more than twice the levels of succinate, L-DELTA1-pyrroline-5-carboxylate, or guanidine generated | Pseudomonas savastanoi pv. phaseolicola PK2 | ? | - |
? | |
| 1.14.20.7 | 2-oxoadipate + L-arginine + O2 | - |
Pseudomonas savastanoi pv. phaseolicola | glutarate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O | - |
? | |
| 1.14.20.7 | 2-oxoadipate + L-arginine + O2 | - |
Pseudomonas savastanoi pv. phaseolicola PK2 | glutarate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O | - |
? | |
| 1.14.20.7 | 2-oxoglutarate + L-arginine + O2 | - |
Pseudomonas savastanoi pv. phaseolicola | succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O | - |
? | |
| 1.14.20.7 | 2-oxoglutarate + L-arginine + O2 | - |
Pseudomonas savastanoi pv. phaseolicola PK2 | succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O | - |
? | |
| 1.14.20.7 | additional information | cf. EC 1.13.12.19, ethylene production. Selected L-Arg derivatives induce ethylene formation without undergoing hydroxylation, demonstrating that ethylen production and L-Arg hydroxylation activities are not linked. Enzyme EFE utilizes the alternative 2-oxo acid 2-oxoadipate as a cosubstrate (forming glutaric acid) during the hydroxylation of L-Arg, with this reaction unlinked from ethylene formation. The amount of ethylene produced is more than twice the levels of succinate, L-DELTA1-pyrroline-5-carboxylate, or guanidine generated | Pseudomonas savastanoi pv. phaseolicola | ? | - |
? | |
| 1.14.20.7 | additional information | cf. EC 1.13.12.19, ethylene production. Selected L-Arg derivatives induce ethylene formation without undergoing hydroxylation, demonstrating that ethylen production and L-Arg hydroxylation activities are not linked. Enzyme EFE utilizes the alternative 2-oxo acid 2-oxoadipate as a cosubstrate (forming glutaric acid) during the hydroxylation of L-Arg, with this reaction unlinked from ethylene formation. The amount of ethylene produced is more than twice the levels of succinate, L-DELTA1-pyrroline-5-carboxylate, or guanidine generated | Pseudomonas savastanoi pv. phaseolicola PK2 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.13.12.19 | monomer | 1 * 40000, recombinant detagged enzyme, SDS-PAGE, 1 * 39670, recombinant detagged enzyme, mass spectrometry | Pseudomonas savastanoi pv. phaseolicola |
| 1.14.20.7 | monomer | 1 * 40000, recombinant detagged enzyme, SDS-PAGE, 1 * 39670, recombinant detagged enzyme, mass spectrometry | Pseudomonas savastanoi pv. phaseolicola |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.13.12.19 | ethylene-forming enzyme | - |
Pseudomonas savastanoi pv. phaseolicola |
| 1.13.12.19 | More | cf. EC 1.14.11.34 | Pseudomonas savastanoi pv. phaseolicola |
| 1.14.20.7 | 2-oxoglutarate-Fe(II) oxygenase | - |
Pseudomonas savastanoi pv. phaseolicola |
| 1.14.20.7 | 2OG-Fe(II) oxygenase | - |
Pseudomonas savastanoi pv. phaseolicola |
| 1.14.20.7 | EFE | - |
Pseudomonas savastanoi pv. phaseolicola |
| 1.14.20.7 | ethylene-forming enzyme | - |
Pseudomonas savastanoi pv. phaseolicola |
| 1.14.20.7 | More | cf. EC 1.13.12.19 | Pseudomonas savastanoi pv. phaseolicola |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.19 | 25 | - |
assay at | Pseudomonas savastanoi pv. phaseolicola |
| 1.14.20.7 | 25 | - |
assay at | Pseudomonas savastanoi pv. phaseolicola |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.20.7 | 0.0042 | - |
2-oxoadipate | pH 7.5, 25°C, recombinant enzyme | Pseudomonas savastanoi pv. phaseolicola | |
| 1.14.20.7 | 0.0045 | - |
L-arginine | with 2-oxoadipate as cosubstrate, at pH 7.5 and 25°C | Pseudomonas savastanoi pv. phaseolicola | |
| 1.14.20.7 | 0.048 | - |
L-arginine | at pH 7.5 and 25°C | Pseudomonas savastanoi pv. phaseolicola | |
| 1.14.20.7 | 2.067 | - |
2-oxoglutarate | pH 7.5, 25°C, recombinant enzyme | Pseudomonas savastanoi pv. phaseolicola | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.19 | 6 | 7.5 | broad optimum | Pseudomonas savastanoi pv. phaseolicola |
| 1.14.20.7 | 6 | 7.5 | broad optimum | Pseudomonas savastanoi pv. phaseolicola |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.19 | 6 | 9 | over 50% of maximal activity within this range | Pseudomonas savastanoi pv. phaseolicola |
| 1.14.20.7 | 6 | 9 | over 50% of maximal activity within this range | Pseudomonas savastanoi pv. phaseolicola |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.13.12.19 | evolution | ethylene-forming enzyme (EFE) is a member of the mononuclear non-heme Fe(II)- and 2-oxoglutarate (2OG)-dependent oxygenase superfamily | Pseudomonas savastanoi pv. phaseolicola |
| 1.13.12.19 | physiological function | the enzyme is reported to simultaneously catalyze the conversion of 2OG into ethylene plus three CO2 and the Cdelta hydroxylation of L-arginine (L-Arg) while oxidatively decarboxylating 2OG to form succinate and carbon dioxide. The enzyme produces ethylene, a gas that is widely used as a building block in the production of various plastics, detergents, surfactants, antifreeze, solvents, and other important industrial materials. And ethylene is a plant hormone that plays an important role in growth and development. The ethylene-forming reaction is not intrinsically linked to L-Arg hydroxylation | Pseudomonas savastanoi pv. phaseolicola |
| 1.14.20.7 | evolution | ethylene-forming enzyme (EFE) is a member of the mononuclear non-heme Fe(II)- and 2-oxoglutarate (2OG)-dependent oxygenase superfamily | Pseudomonas savastanoi pv. phaseolicola |
| 1.14.20.7 | physiological function | the enzyme is reported to simultaneously catalyze the conversion of 2OG into ethylene plus three CO2 and the Cdelta hydroxylation of L-arginine (L-Arg) while oxidatively decarboxylating 2-oxoglutarate to form succinate and carbon dioxide. The enzyme produces ethylene, a gas that is widely used as a building block in the production of various plastics, detergents, surfactants, antifreeze, solvents, and other important industrial materials. And ethylene is a plant hormone that plays an important role in growth and development. The ethylene-forming reaction is not intrinsically linked to L-Arg hydroxylation | Pseudomonas savastanoi pv. phaseolicola |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.20.7 | 0.134 | - |
2-oxoadipate | pH 7.5, 25°C, recombinant enzyme | Pseudomonas savastanoi pv. phaseolicola | |
| 1.14.20.7 | 0.97 | - |
L-arginine | at pH 7.5 and 25°C | Pseudomonas savastanoi pv. phaseolicola | |
| 1.14.20.7 | 36.26 | - |
2-oxoglutarate | pH 7.5, 25°C, recombinant enzyme | Pseudomonas savastanoi pv. phaseolicola | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
