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Literature summary extracted from

  • Chen, Y.; Ji, W.; Zhang, H.; Zhang, X.; Yu, Y.
    Cloning, expression and characterization of a putative 2,5-diketo-D-gluconic acid reductase in Comamonas testosteroni (2015), Chem. Biol. Interact., 234, 229-235 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.274 gene CTATCC11996_22452, genetic organization, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Comamonas testosteroni
1.1.1.346 gene CTATCC11996_22452, genetic organization, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Comamonas testosteroni

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.274 additional information construction of enzyme gene knockout mutant M-AKR, that shows decreased degradation activity with testosterone, estradiol, oestrone, and methyltestosterone compared to the wild-type enzyme. Compared to the wild-type, the mutation of the endogenous 2,5DKR gene results in lower degradation of estradiol and methyltestosterone but has no effct on degradation of estrone and testosterone Comamonas testosteroni
1.1.1.346 additional information construction of enzyme gene knockout mutant M-AKR, that shows decreased degradation activity with testosterone, estradiol, oestrone, and methyltestosterone compared to the wild-type enzyme. Compared to the wild-type, the mutation of the endogenous 2,5DKR gene results in lower degradation of estradiol and methyltestosterone but has no effct on degradation of estrone and testosterone Comamonas testosteroni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.274 2-dehydro-D-gulonate + NADP+ Comamonas testosteroni
-
 2,5-didehydro-D-gluconate + NADPH + H+
-
 ?
1.1.1.274 2-dehydro-D-gulonate + NADP+ Comamonas testosteroni ATCC 11996
-
 2,5-didehydro-D-gluconate + NADPH + H+
-
 ?
1.1.1.274 additional information Comamonas testosteroni the enzyme catalyzes degradation of estradiol, estrone, testosterone, and methyltestosterone, overview ?
-
 ?
1.1.1.274 additional information Comamonas testosteroni ATCC 11996 the enzyme catalyzes degradation of estradiol, estrone, testosterone, and methyltestosterone, overview ?
-
 ?
1.1.1.346 2-dehydro-L-gulonate + NADP+ Comamonas testosteroni
-
 2,5-didehydro-D-gluconate + NADPH + H+
-
 ?
1.1.1.346 2-dehydro-L-gulonate + NADP+ Comamonas testosteroni ATCC 11996
-
 2,5-didehydro-D-gluconate + NADPH + H+
-
 ?
1.1.1.346 additional information Comamonas testosteroni the enzyme catalyzes degradation of estradiol, oestrone, testosterone, and methyltestosterone, overview ?
-
 ?
1.1.1.346 additional information Comamonas testosteroni ATCC 11996 the enzyme catalyzes degradation of estradiol, oestrone, testosterone, and methyltestosterone, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.274 Comamonas testosteroni
-
-
-
1.1.1.274 Comamonas testosteroni ATCC 11996
-
-
-
1.1.1.346 Comamonas testosteroni
-
-
-
1.1.1.346 Comamonas testosteroni ATCC 11996
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.274 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Comamonas testosteroni
1.1.1.346 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Comamonas testosteroni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.274 2-dehydro-D-gulonate + NADP+
-
 Comamonas testosteroni 2,5-didehydro-D-gluconate + NADPH + H+
-
 ?
1.1.1.274 2-dehydro-D-gulonate + NADP+
-
 Comamonas testosteroni ATCC 11996 2,5-didehydro-D-gluconate + NADPH + H+
-
 ?
1.1.1.274 additional information the enzyme catalyzes degradation of estradiol, estrone, testosterone, and methyltestosterone, overview Comamonas testosteroni ?
-
 ?
1.1.1.274 additional information the enzyme catalyzes degradation of estradiol, estrone, testosterone, and methyltestosterone, overview Comamonas testosteroni ATCC 11996 ?
-
 ?
1.1.1.346 2-dehydro-L-gulonate + NADP+
-
 Comamonas testosteroni 2,5-didehydro-D-gluconate + NADPH + H+
-
 ?
1.1.1.346 2-dehydro-L-gulonate + NADP+
-
 Comamonas testosteroni ATCC 11996 2,5-didehydro-D-gluconate + NADPH + H+
-
 ?
1.1.1.346 additional information the enzyme catalyzes degradation of estradiol, oestrone, testosterone, and methyltestosterone, overview Comamonas testosteroni ?
-
 ?
1.1.1.346 additional information the enzyme catalyzes degradation of estradiol, oestrone, testosterone, and methyltestosterone, overview Comamonas testosteroni ATCC 11996 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.274 ? x * 39400, about, sequence calculation, x * 41600, recombinant His-tagged enzyme, SDS-PAGE Comamonas testosteroni
1.1.1.346 ? x * 39400, about, sequence calculation, x * 41600, recombinant His-tagged enzyme, SDS-PAGE Comamonas testosteroni

Synonyms

EC Number Synonyms Comment Organism
1.1.1.274 2,5-diketo-D-gluconic acid reductase
-
 Comamonas testosteroni
1.1.1.274 2,5DKGR
-
 Comamonas testosteroni
1.1.1.274 CTATCC11996_22452
-
 Comamonas testosteroni
1.1.1.346 2,5-diketo-D-gluconic acid reductase
-
 Comamonas testosteroni
1.1.1.346 2,5DKGR
-
 Comamonas testosteroni
1.1.1.346 CTATCC11996_22452
-
 Comamonas testosteroni

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.274 NADP+
-
 Comamonas testosteroni
1.1.1.346 NADP+
-
 Comamonas testosteroni

Expression

EC Number Organism Comment Expression
1.1.1.274 Comamonas testosteroni the enzyme expression is significantly induced by estrone, estradiol, and methyltestosterone, and slightly by progesterone, but not by testosterone up
1.1.1.346 Comamonas testosteroni the enzyme expression is significantly induced by estrone, estradiol, and methyltestosterone, and slightly by progesterone, but not by testosterone up

General Information

EC Number General Information Comment Organism
1.1.1.274 evolution the enzyme belongs to the AKR superfamily, monomeric (alpha/beta) 8-barrel proteins which bind NAD(P)(H) to metabolize an array of substrates Comamonas testosteroni
1.1.1.274 malfunction compared to the wild-type, the knockout mutation of the endogenous 2,5DKR gene results in lower degradation of estradiol and methyltestosterone but has no effct on degradation of estrone and testosterone. Cell growth on ethanol, oestrone, estradiol, testosterone or methyltestosterone is reduced in the mutant strain compared to the wild-type Comamonas testosteroni
1.1.1.274 additional information three consensus sequences of the AKR superfamily are found as GxxxxDxAxxY, LxxxGxxxPxxGxG and LxxxxxxxxxDxxxxH. GxxxxDxAxxY is the active site, LxxxGxxxPxxGxG is the cofactor-binding site for NAD(P)(H), and LxxxxxxxxxDxxxxH is required for supporting the 3D structure Comamonas testosteroni
1.1.1.346 evolution the enzyme belongs to the AKR superfamily, monomeric (alpha/beta) 8-barrel proteins which bind NAD(P)(H) to metabolize an array of substrates Comamonas testosteroni
1.1.1.346 malfunction compared to the wild-type, the knockout mutation of the endogenous 2,5DKR gene results in lower degradation of estradiol and methyltestosterone but has no effct on degradation of estrone and testosterone. Cell growth on ethanol, oestrone, estradiol, testosterone or methyltestosterone is reduced in the mutant strain compared to the wild-type Comamonas testosteroni
1.1.1.346 additional information three consensus sequences of the AKR superfamily are found as GxxxxDxAxxY, LxxxGxxxPxxGxG and LxxxxxxxxxDxxxxH. GxxxxDxAxxY is the active site, LxxxGxxxPxxGxG is the cofactor-binding site for NAD(P)(H), and LxxxxxxxxxDxxxxH is required for supporting the 3D structure Comamonas testosteroni