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Literature summary extracted from

  • Flanagan, L.A.; Wright, J.J.; Roessler, M.M.; Moir, J.W.; Parkin, A.
    Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance (2016), Chem. Commun. (Camb.), 52, 9133-9136 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.12.99.6 expressed in Escherichia coli Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.12.99.6 E73A the catalytic activity of the mutant is comparable to native enzyme Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.12.99.6 O2
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.99.6 H2 + methylene blue Escherichia coli
-
 reduced methylene blue
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.12.99.6 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.99.6 HisTrap Ni affinity column chromatography, and Superdex 200 gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.99.6 H2 + methylene blue
-
 Escherichia coli reduced methylene blue
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.12.99.6 Hyd-1
-
 Escherichia coli
1.12.99.6 hydrogenase-1
-
 Escherichia coli
1.12.99.6 NiFe hydrogenase
-
 Escherichia coli