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Literature summary extracted from

  • Knop, M.; Lemnaru, R.; Seebeck, F.P.
    Mutation of conserved residues increases in vitro activity of the formylglycine-generating enzyme (2017), ChemBioChem, 18, 1755-1761 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.8.3.7 C187A the mutation leads to an increase of enzyme activity Thermomonospora curvata
1.8.3.7 C187A/C231A/C284S/C298A the mutations lead to an increase of enzyme activity Thermomonospora curvata
1.8.3.7 C187A/C231A/Y273F/C284S/C298A the mutations lead to an increase of enzyme activity Thermomonospora curvata
1.8.3.7 C187A/C284S/C298A the mutations lead to an increase of enzyme activity Thermomonospora curvata
1.8.3.7 C298A the in vitro activity is not affected by the mutation Thermomonospora curvata
1.8.3.7 Y273F the mutation leads to an increase of enzyme activity Thermomonospora curvata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.3.7 0.1
-
 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 mutant C187A/C231A/Y273F/C284S/C298A, at pH 8.0 and 25°C Thermomonospora curvata
1.8.3.7 0.21
-
 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 mutant C187A/C231A/C284S/C298A, at pH 8.0 and 25°C Thermomonospora curvata
1.8.3.7 0.28
-
 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 mutant C187A/C284S/C298A, at pH 8.0 and 25°C Thermomonospora curvata
1.8.3.7 0.5
-
 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 mutant Y273F, at pH 8.0 and 25°C Thermomonospora curvata
1.8.3.7 0.55
-
 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 wild type enzyme, at pH 8.0 and 25°C Thermomonospora curvata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.3.7 Cu2+ 0.002 mM used in assay conditions Thermomonospora curvata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.3.7 a [sulfatase]-L-cysteine + O2 + a thiol Thermomonospora curvata
-
 a [sulfatase]-3-oxo-L-alanine + hydrogen sulfide + a disulfide + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.3.7 Thermomonospora curvata
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.3.7 a [sulfatase]-L-cysteine + O2 + a thiol
-
 Thermomonospora curvata a [sulfatase]-3-oxo-L-alanine + hydrogen sulfide + a disulfide + H2O
-
 ?
1.8.3.7 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 + O2 + hydrogen sulfide + oxidized dithiothreitol
-
 Thermomonospora curvata ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.3.7 FGE
-
 Thermomonospora curvata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.3.7 0.027
-
 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 wild type enzyme, at pH 8.0 and 25°C Thermomonospora curvata
1.8.3.7 0.065
-
 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 mutant C187A/C284S/C298A, at pH 8.0 and 25°C Thermomonospora curvata
1.8.3.7 0.07
-
 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 mutant C187A/C231A/C284S/C298A, at pH 8.0 and 25°C Thermomonospora curvata
1.8.3.7 0.17
-
 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 mutant Y273F, at pH 8.0 and 25°C Thermomonospora curvata
1.8.3.7 0.18
-
 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 mutant C187A/C231A/Y273F/C284S/C298A, at pH 8.0 and 25°C Thermomonospora curvata

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.3.7 0.0483
-
 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 wild type enzyme, at pH 8.0 and 25°C Thermomonospora curvata
1.8.3.7 0.233
-
 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 mutant C187A/C284S/C298A, at pH 8.0 and 25°C Thermomonospora curvata
1.8.3.7 0.316
-
 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 mutant Y273F, at pH 8.0 and 25°C Thermomonospora curvata
1.8.3.7 0.333
-
 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 mutant C187A/C231A/C284S/C298A, at pH 8.0 and 25°C Thermomonospora curvata
1.8.3.7 1.833
-
 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 mutant C187A/C231A/Y273F/C284S/C298A, at pH 8.0 and 25°C Thermomonospora curvata