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Literature summary extracted from

  • Wakamatsu, T.; Higashi, C.; Ohmori, T.; Doi, K.; Ohshima, T.
    Biochemical characterization of two glutamate dehydrogenases with different cofactor specificities from a hyperthermophilic archaeon Pyrobaculum calidifontis (2013), Extremophiles, 17, 379-389 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.2 isocitrate 10 M, 112% of initial activity Pyrobaculum calidifontis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.2 malate 10 mM, 75% residual activity Pyrobaculum calidifontis
1.4.1.2 oxaloacetate 10 mM, 60% residual activity Pyrobaculum calidifontis
1.4.1.4 Hg2+ 1 mM, 76% residual activity Pyrobaculum calidifontis
1.4.1.4 isocitrate 10 M, 64% of initial activity Pyrobaculum calidifontis
1.4.1.4 KCl 100 mM, 45% residual activity Pyrobaculum calidifontis
1.4.1.4 NaCl 100 mM, 59% residual activity Pyrobaculum calidifontis
1.4.1.4 oxaloacetate 10 mM, 63% residual activity Pyrobaculum calidifontis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.2 0.01
-
 NADH pH 9.5, 50°C Pyrobaculum calidifontis
1.4.1.2 0.028
-
 NAD+ pH 10.5, 50°C Pyrobaculum calidifontis
1.4.1.2 0.92
-
 2-oxoglutarate pH 9.5, 50°C Pyrobaculum calidifontis
1.4.1.2 5.3
-
 L-glutamate pH 10.5, 50°C Pyrobaculum calidifontis
1.4.1.2 9.8
-
 NH3 pH 9.5, 50°C Pyrobaculum calidifontis
1.4.1.4 0.017
-
 NADPH pH 9.0, 50°C Pyrobaculum calidifontis
1.4.1.4 0.035
-
 NADP+ pH 9.5, 50°C Pyrobaculum calidifontis
1.4.1.4 1.7
-
 2-oxoglutarate pH 9.0, 50°C Pyrobaculum calidifontis
1.4.1.4 2.2
-
 NH3 pH 9.0, 50°C Pyrobaculum calidifontis
1.4.1.4 3.4
-
 L-glutamate pH 9.5, 50°C Pyrobaculum calidifontis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.1.2 Mn2+ 1 mM, 109% of initial activity Pyrobaculum calidifontis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.2 286000
-
 gel filtration Pyrobaculum calidifontis
1.4.1.4 270000
-
 gel filtration Pyrobaculum calidifontis

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.2 Pyrobaculum calidifontis A3MUY9
-
-
1.4.1.2 Pyrobaculum calidifontis JCM 11548 A3MUY9
-
-
1.4.1.4 Pyrobaculum calidifontis A3MWK6
-
-
1.4.1.4 Pyrobaculum calidifontis JCM 11548 A3MWK6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.2 2-oxobutanoate + NH3 + NADH + H+ 2.7% of the activity with 2-oxoglutarate Pyrobaculum calidifontis L-2-aminobutanoate + H2O + NAD+
-
 r
1.4.1.2 2-oxobutanoate + NH3 + NADH + H+ 2.7% of the activity with 2-oxoglutarate Pyrobaculum calidifontis JCM 11548 L-2-aminobutanoate + H2O + NAD+
-
 r
1.4.1.2 2-oxocaproate + NH3 + NADH + H+ 1.3% of the activity with 2-oxoglutarate Pyrobaculum calidifontis L-2-aminohexanoate + H2O + NAD+
-
 r
1.4.1.2 2-oxoglutarate + NH3 + NADH + H+
-
 Pyrobaculum calidifontis L-glutamate + H2O + NAD+
-
 r
1.4.1.2 2-oxoglutarate + NH3 + NADH + H+
-
 Pyrobaculum calidifontis JCM 11548 L-glutamate + H2O + NAD+
-
 r
1.4.1.2 2-oxovalerate + NH3 + NADH + H+ 6.6% of the activity with 2-oxoglutarate Pyrobaculum calidifontis L-valine + H2O + NAD+
-
 r
1.4.1.2 L-2-aminobutanoate + H2O + NAD+ 8.4% of the activity with L-glutamate Pyrobaculum calidifontis 2-oxobutanoate + NH3 + NADH + H+
-
 r
1.4.1.2 L-2-aminobutanoate + H2O + NAD+ 8.4% of the activity with L-glutamate Pyrobaculum calidifontis JCM 11548 2-oxobutanoate + NH3 + NADH + H+
-
 r
1.4.1.2 L-glutamate + H2O + NAD+
-
 Pyrobaculum calidifontis 2-oxoglutarate + NH3 + NADH + H+
-
 r
1.4.1.2 L-glutamate + H2O + NAD+
-
 Pyrobaculum calidifontis JCM 11548 2-oxoglutarate + NH3 + NADH + H+
-
 r
1.4.1.2 L-isoleucine + H2O + NAD+ 3.0% of the activity with L-glutamate Pyrobaculum calidifontis 2-oxo-3-methylvalerate + NH3 + NADH + H+
-
 r
1.4.1.2 L-leucine + H2O + NAD+ 1.6% of the activity with L-glutamate Pyrobaculum calidifontis 2-oxo-iso-valerate + NH3 + NADH + H+
-
 r
1.4.1.2 L-norvaline + H2O + NAD+ 35% of the activity with L-glutamate Pyrobaculum calidifontis 2-oxopentanoate + NH3 + NADH + H+
-
 r
1.4.1.2 L-norvaline + H2O + NAD+ 35% of the activity with L-glutamate Pyrobaculum calidifontis JCM 11548 2-oxopentanoate + NH3 + NADH + H+
-
 r
1.4.1.2 L-valine + H2O + NAD+ 31% of the activity with L-glutamate Pyrobaculum calidifontis 2-oxovalerate + NH3 + NADH + H+
-
 r
1.4.1.4 2-oxobutanoate + NH3 + NADPH + H+ 2.6% of the activity with 2-oxoglutarate Pyrobaculum calidifontis L-2-aminobutanoate + H2O + NADP+
-
 r
1.4.1.4 2-oxobutanoate + NH3 + NADPH + H+ 2.6% of the activity with 2-oxoglutarate Pyrobaculum calidifontis JCM 11548 L-2-aminobutanoate + H2O + NADP+
-
 r
1.4.1.4 2-oxoglutarate + NH3 + NADPH + H+
-
 Pyrobaculum calidifontis L-glutamate + H2O + NADP+
-
 r
1.4.1.4 2-oxoglutarate + NH3 + NADPH + H+
-
 Pyrobaculum calidifontis JCM 11548 L-glutamate + H2O + NADP+
-
 r
1.4.1.4 2-oxovalerate + NH3 + NADPH + H+ 4.2% of the activity with 2-oxoglutarate Pyrobaculum calidifontis L-valine + H2O + NADP+
-
 r
1.4.1.4 2-oxovalerate + NH3 + NADPH + H+ 4.2% of the activity with 2-oxoglutarate Pyrobaculum calidifontis JCM 11548 L-valine + H2O + NADP+
-
 r
1.4.1.4 L-glutamate + H2O + NADP+
-
 Pyrobaculum calidifontis 2-oxoglutarate + NH3 + NADPH + H+
-
 r
1.4.1.4 L-glutamate + H2O + NADP+
-
 Pyrobaculum calidifontis JCM 11548 2-oxoglutarate + NH3 + NADPH + H+
-
 r
1.4.1.4 L-norvaline + H2O + NADP+ 3% of the activity with L-glutamate Pyrobaculum calidifontis 2-oxopentanoate + NH3 + NADPH + H+
-
 r
1.4.1.4 L-norvaline + H2O + NADP+ 3% of the activity with L-glutamate Pyrobaculum calidifontis JCM 11548 2-oxopentanoate + NH3 + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.2 hexamer 6 * 48200, calculated Pyrobaculum calidifontis
1.4.1.4 hexamer 6 * 47700, calculated Pyrobaculum calidifontis

Synonyms

EC Number Synonyms Comment Organism
1.4.1.2 Pcal_1031
-
 Pyrobaculum calidifontis
1.4.1.4 Pcal_1606
-
 Pyrobaculum calidifontis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.1.2 14
-
 NAD+ pH 10.5, 50°C Pyrobaculum calidifontis
1.4.1.2 17
-
 L-glutamate pH 10.5, 50°C Pyrobaculum calidifontis
1.4.1.2 58
-
 NADH pH 9.5, 50°C Pyrobaculum calidifontis
1.4.1.2 58
-
 NH3 pH 9.5, 50°C Pyrobaculum calidifontis
1.4.1.2 65
-
 2-oxoglutarate pH 9.5, 50°C Pyrobaculum calidifontis
1.4.1.4 9.9
-
 NADP+ pH 9.5, 50°C Pyrobaculum calidifontis
1.4.1.4 13
-
 L-glutamate pH 9.5, 50°C Pyrobaculum calidifontis
1.4.1.4 92
-
 NADPH pH 9.0, 50°C Pyrobaculum calidifontis
1.4.1.4 95
-
 2-oxoglutarate pH 9.0, 50°C Pyrobaculum calidifontis
1.4.1.4 100
-
 NH3 pH 9.0, 50°C Pyrobaculum calidifontis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.2 additional information no cofactor: NADP+ Pyrobaculum calidifontis
1.4.1.2 NAD+
-
 Pyrobaculum calidifontis
1.4.1.2 NADH
-
 Pyrobaculum calidifontis
1.4.1.2 NADPH about 1% of th activity with NADH Pyrobaculum calidifontis
1.4.1.4 NAD+ about 17% of the activity with NADP+ Pyrobaculum calidifontis
1.4.1.4 NADH about 7% of the activity with NADPH Pyrobaculum calidifontis
1.4.1.4 NADP+
-
 Pyrobaculum calidifontis
1.4.1.4 NADPH
-
 Pyrobaculum calidifontis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.1.2 3.2
-
 L-glutamate pH 10.5, 50°C Pyrobaculum calidifontis
1.4.1.2 5.9
-
 NH3 pH 9.5, 50°C Pyrobaculum calidifontis
1.4.1.2 71
-
 2-oxoglutarate pH 9.5, 50°C Pyrobaculum calidifontis
1.4.1.2 500
-
 NAD+ pH 10.5, 50°C Pyrobaculum calidifontis
1.4.1.2 5800
-
 NADH pH 9.5, 50°C Pyrobaculum calidifontis
1.4.1.4 3.8
-
 L-glutamate pH 9.5, 50°C Pyrobaculum calidifontis
1.4.1.4 45
-
 NH3 pH 9.0, 50°C Pyrobaculum calidifontis
1.4.1.4 56
-
 2-oxoglutarate pH 9.0, 50°C Pyrobaculum calidifontis
1.4.1.4 280
-
 NADP+ pH 9.5, 50°C Pyrobaculum calidifontis
1.4.1.4 5400
-
 NADPH pH 9.0, 50°C Pyrobaculum calidifontis