EC Number | Application | Comment | Organism |
---|---|---|---|
1.14.99.63 | synthesis | construction of an astaxanthin biosynthesis pathway in Saccharomyces cerevisiae by introducing heterologous beta-carotene hydroxylase (CrtZ) and beta-carotene ketolase (CrtW) into an existing high beta-carotene producing strain. Astaxanthin yield of 3.1 mg/g dry cell weight are achieved. After change of promoter and hrough high cell density fed-batch fermentation using a carbon source restriction strategy, the production of astaxanthin in a 5-L bioreactor reaches to 81.0 mg/l | Brevundimonas vesicularis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.11.B16 | expressed in Saccharomyces cerevisiae strain Sc118030 | Brevundimonas vesicularis |
1.14.15.24 | astaxanthin biosynthesis pathway is constructed in Saccharomyces cerevisiae by introducing heterologous CrtZ and CrtW into an existing high beta-carotene producing strain. Both genes crtZ and crtW are codon optimized and expressed under the control of constitutive promoters. Strain SyBE_Sc118060 with CrtW from Brevundimonas vesicularis DC263 and CrtZ from Alcaligenes sp. strain PC-1 achieves the highest astaxanthin yield of 3.1 mg/g dry cell weight | Alcaligenes sp. PC-1 |
1.14.15.24 | expressed in Saccharomyces cerevisiae | Alcaligenes sp. PC-1 |
1.14.99.63 | astaxanthin biosynthesis pathway is constructed in Saccharomyces cerevisiae by introducing heterologous CrtZ and CrtW into an existing high beta-carotene producing strain. Both genes crtZ and crtW are codon optimized and expressed under the control of constitutive promoters. Strain SyBE_Sc118060 with CrtW from Brevundimonas vesicularis DC263 and CrtZ from Alcaligenes sp. strain PC-1 achieves the highest astaxanthin yield of 3.1 mg/g dry cell weight | Brevundimonas vesicularis |
1.14.99.63 | expression in Saccharomyces cerevisiae | Brevundimonas vesicularis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.11.B16 | beta-carotene + 2-oxoglutarate + O2 + H2O | Brevundimonas vesicularis | - |
canthaxanthin + succinate + CO2 + 4 H+ | - |
? | |
1.14.11.B16 | beta-carotene + 2-oxoglutarate + O2 + H2O | Brevundimonas vesicularis DC263 | - |
canthaxanthin + succinate + CO2 + 4 H+ | - |
? | |
1.14.15.24 | beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | Alcaligenes sp. PC-1 | - |
beta-cryptoxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
1.14.15.24 | beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | Alcaligenes sp. PC-1 | overall reaction | zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
1.14.15.24 | beta-cryptoxanthin + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | Alcaligenes sp. PC-1 | - |
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.11.B16 | Brevundimonas vesicularis | - |
- |
- |
1.14.11.B16 | Brevundimonas vesicularis DC263 | - |
- |
- |
1.14.15.24 | Alcaligenes sp. PC-1 | - |
- |
- |
1.14.15.24 | Alcaligenes sp. PC-1 | Q44262 | - |
- |
1.14.99.63 | Brevundimonas vesicularis | Q0H2C9 | - |
- |
1.14.99.63 | Brevundimonas vesicularis DC263 | Q0H2C9 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.11.B16 | beta-carotene + 2-oxoglutarate + O2 + H2O | - |
Brevundimonas vesicularis | canthaxanthin + succinate + CO2 + 4 H+ | - |
? | |
1.14.11.B16 | beta-carotene + 2-oxoglutarate + O2 + H2O | - |
Brevundimonas vesicularis DC263 | canthaxanthin + succinate + CO2 + 4 H+ | - |
? | |
1.14.15.24 | beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | - |
Alcaligenes sp. PC-1 | beta-cryptoxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
1.14.15.24 | beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | overall reaction | Alcaligenes sp. PC-1 | zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
1.14.15.24 | beta-cryptoxanthin + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | - |
Alcaligenes sp. PC-1 | zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.11.B16 | beta-carotene ketolase | - |
Brevundimonas vesicularis |
1.14.11.B16 | CrtW | - |
Brevundimonas vesicularis |
1.14.15.24 | beta-carotene hydroxylase | - |
Alcaligenes sp. PC-1 |
1.14.15.24 | CrtZ | - |
Alcaligenes sp. PC-1 |
1.14.99.63 | CrtW | - |
Brevundimonas vesicularis |