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Literature summary extracted from
- Identification of a dolabellane type diterpene synthase and other root-expressed diterpene synthases in Arabidopsis (2016), Front. Plant Sci., 7, 1761 .
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.3.167 | 0.0063 | - |
geranylgeranyl diphosphate | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |  |
| 4.2.3.168 | 0.0063 | - |
geranylgeranyl diphosphate | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.2.3.167 | chloroplast | - |
Arabidopsis thaliana | 9507 | - |
| 4.2.3.168 | chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.3.167 | geranylgeranyl diphosphate + H2O | Arabidopsis thaliana | - |
(3E,7E)-dolabella-3,7-dien-18-ol + diphosphate | - |
? | |
| 4.2.3.168 | geranylgeranyl diphosphate + H2O | Arabidopsis thaliana | - |
(3E,7E)-dolathalia-3,7,11-triene + diphosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.3.167 | Arabidopsis thaliana | A0A178U9Y5 | bifunctional dolabella-3,7-dien-18-ol synthase/dolathalia-3,7,11-triene synthase, reactions of EC 4.2.3.167 and EC 4.2.3.168, respectively | - |
| 4.2.3.168 | Arabidopsis thaliana | A0A178U9Y5 | bifunctional dolabella-3,7-dien-18-ol synthase/dolathalia-3,7,11-triene synthase, reactions of EC 4.2.3.167 and EC 4.2.3.168, respectively | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.2.3.167 | flower | ecotype Cvi | Arabidopsis thaliana | - |
| 4.2.3.167 | leaf | ecotype Cvi | Arabidopsis thaliana | - |
| 4.2.3.167 | additional information | TPS20 compounds occur in all tissues of ecotype Cvi but are absent in the Col ecotype because of deletion and substitution mutations in the Col TPS20 sequence | Arabidopsis thaliana | - |
| 4.2.3.167 | root | ecotype Cvi | Arabidopsis thaliana | - |
| 4.2.3.168 | flower | ecotype Cvi | Arabidopsis thaliana | - |
| 4.2.3.168 | leaf | ecotype Cvi | Arabidopsis thaliana | - |
| 4.2.3.168 | additional information | TPS20 compounds occur in all tissues of ecotype Cvi but are absent in the Col ecotype because of deletion and substitution mutations in the Col TPS20 sequence | Arabidopsis thaliana | - |
| 4.2.3.168 | root | ecotype Cvi | Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.3.167 | geranylgeranyl diphosphate + H2O | - |
Arabidopsis thaliana | (3E,7E)-dolabella-3,7-dien-18-ol + diphosphate | - |
? | |
| 4.2.3.167 | additional information | reaction presumably proceeds via dephosphorylation of geranylgeranyl diphosphate and the formation of a dolabelanyl carbocation, which is then converted to dolathaliatriene by a Wagner-Meerwein rearrangement, reaction of EC 4.2.3.168, and deprotonation or directly to dolabelladienol by quenching with water | Arabidopsis thaliana | ? | - |
? | |
| 4.2.3.168 | geranylgeranyl diphosphate + H2O | - |
Arabidopsis thaliana | (3E,7E)-dolathalia-3,7,11-triene + diphosphate | - |
? | |
| 4.2.3.168 | additional information | reaction presumably proceeds via dephosphorylation of geranylgeranyl diphosphate and the formation of a dolabelanyl carbocation, which is then converted to dolathaliatriene by a Wagner-Meerwein rearrangement and deprotonation or directly to dolabelladienol by quenching with water, reaction of EC 4.2.3.167 | Arabidopsis thaliana | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.3.167 | At5g48110 | - |
Arabidopsis thaliana |
| 4.2.3.167 | TPS20 | - |
Arabidopsis thaliana |
| 4.2.3.168 | At5g48110 | - |
Arabidopsis thaliana |
| 4.2.3.168 | TPS20 | - |
Arabidopsis thaliana |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.3.167 | 0.83 | - |
geranylgeranyl diphosphate | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 4.2.3.168 | 0.83 | - |
geranylgeranyl diphosphate | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 4.2.3.167 | physiological function | the primary TPS20 diterpene products retard the growth of the root rot pathogen Pythium irregulare but only at concentrations exceeding those in planta | Arabidopsis thaliana |
| 4.2.3.168 | physiological function | the primary TPS20 diterpene products retard the growth of the root rot pathogen Pythium irregulare but only at concentrations exceeding those in planta | Arabidopsis thaliana |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.3.167 | 130 | - |
geranylgeranyl diphosphate | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 4.2.3.168 | 130 | - |
geranylgeranyl diphosphate | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
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Release 2023.1 (January 2023)
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