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Literature summary extracted from

  • Tong, Y.; Xin, Y.; Yang, H.; Zhang, L.; Wang, W.
    Efficient improvement on stability of sarcosine oxidase via poly-lysine modification on enzyme surface (2014), Int. J. Biol. Macromol., 67, 140-146 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.3.1 expression in Escherichia coli strain BL21 Bacillus sp. BSD-8

General Stability

EC Number General Stability Organism
1.5.3.1 modified technique is successfully established for efficiently improving the stability of sarcosine oxidase via poly-Lys covalent binding onto enzymatic surface. Compared to the free enzyme, higher pH stability and thermostability of modified sarcosine oxidase is achieved with a series of characterization analysis. It is owing to that the loosening of three-dimensional configuration can be restricted by the enzymatic surface protection with poly-Lys and subsequently maintained a more stable enzyme structure. The changed microenvironment around the enzyme in themodification process can also contribute to the better stability Bacillus sp. BSD-8

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.1 47.94
-
 sarcosine 37°C, pH 8.0, poly-lysine modified enzyme Bacillus sp. BSD-8
1.5.3.1 141.65
-
 sarcosine 37°C, pH 8.0, soluble enzyme Bacillus sp. BSD-8

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.3.1 43800
-
 soluble enzyme, mass spectrum analysis Bacillus sp. BSD-8
1.5.3.1 48000
-
 and 51700, modified enzyme covalently bound with about two or three poly-Lys, mass spectrum analysis Bacillus sp. BSD-8
1.5.3.1 51700
-
 and 48000, modified enzyme covalently bound with about two or three poly-Lys, mass spectrum analysis Bacillus sp. BSD-8

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.1 sarcosine + H2O + O2 Bacillus sp. BSD-8
-
 glycine + formaldehyde + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.1 Bacillus sp. BSD-8 Q6ITC6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.1
-
 Bacillus sp. BSD-8

Storage Stability

EC Number Storage Stability Organism
1.5.3.1 37°C, pH 8.0, 7 days, residual activity of soluble enzyme is 7.9%, residual activity poly-lysine modified enzyme is 95.6% Bacillus sp. BSD-8

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.1 sarcosine + H2O + O2
-
 Bacillus sp. BSD-8 glycine + formaldehyde + H2O2
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.3.1 37
-
 pH 8.0, 7 days, residual activity of soluble enzyme is 7.9%, residual activity poly-lysine modified enzyme is 95.6% Bacillus sp. BSD-8
1.5.3.1 60
-
 10 min, residual activity of soluble enzyme is 2.3%, residual activity poly-lysine modified enzymeis 74.7% Bacillus sp. BSD-8

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.5.3.1 5 10 37°C, 2.5 h, more than 90% of initial activity of poly-lysine modified enzyme is maintained Bacillus sp. BSD-8