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Literature summary extracted from

  • Choi, S.K.; Schurig-Briccio, L.; Ding, Z.; Hong, S.; Sun, C.; Gennis, R.B.
    Location of the substrate binding site of the cytochrome bo3 ubiquinol oxidase from Escherichia coli (2017), J. Am. Chem. Soc., 139, 8346-8354 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.1.3 expressed in Escherichia coli C43(DE3) cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
7.1.1.3 D75E the mutant shows 48% activity with ubiquinol-1 and 88% activity with ubiquinol-2 compared to the wild type enzyme Escherichia coli
7.1.1.3 F93A the mutant shows 37% activity with ubiquinol-1 and 102% activity with ubiquinol-2 compared to the wild type enzyme Escherichia coli
7.1.1.3 F93Y the mutant shows 81% activity with ubiquinol-1 and 107% activity with ubiquinol-2 compared to the wild type enzyme Escherichia coli
7.1.1.3 H98N the mutant shows 3% activity with ubiquinol-1 and 10% activity with ubiquinol-2 compared to the wild type enzyme Escherichia coli
7.1.1.3 H98T the mutant shows 4% activity with ubiquinol-1 and 9% activity with ubiquinol-2 compared to the wild type enzyme Escherichia coli
7.1.1.3 L160W the mutant shows 58% activity with ubiquinol-1 and no activity with ubiquinol-2 compared to the wild type enzyme Escherichia coli
7.1.1.3 N157V the mutant shows 23% activity with ubiquinol-1 and no activity with ubiquinol-2 compared to the wild type enzyme Escherichia coli
7.1.1.3 Q101A the mutant shows 26% activity with ubiquinol-1 and 82% activity with ubiquinol-2 compared to the wild type enzyme Escherichia coli
7.1.1.3 Q101E the mutant shows 55% activity with ubiquinol-1 and no activity with ubiquinol-2 compared to the wild type enzyme Escherichia coli
7.1.1.3 Q101L the mutant shows 11% activity with ubiquinol-1 and 58% activity with ubiquinol-2 compared to the wild type enzyme Escherichia coli
7.1.1.3 Q101M the mutant shows 51% activity with ubiquinol-1 and 72% activity with ubiquinol-2 compared to the wild type enzyme Escherichia coli
7.1.1.3 Q101N the mutant shows 10% activity with ubiquinol-1 and 61% activity with ubiquinol-2 compared to the wild type enzyme Escherichia coli
7.1.1.3 Q101T the mutant shows 27% activity with ubiquinol-1 and 62% activity with ubiquinol-2 compared to the wild type enzyme Escherichia coli
7.1.1.3 Q82A the mutant shows 88% activity with ubiquinol-1 and no activity with ubiquinol-2 compared to the wild type enzyme Escherichia coli
7.1.1.3 R71Q the mutant shows 3% activity with ubiquinol-1 and 8% activity with ubiquinol-2 compared to the wild type enzyme Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.3 2-n-heptyl-4-hydroxyquinoline N-oxide
-
 Escherichia coli
7.1.1.3 aurachin C1-10
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.1.3 0.016
-
 ubiquinol-1 mutant enzyme D75E, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.024
-
 ubiquinol-1 mutant enzyme Q101M, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.03
-
 ubiquinol-1 mutant enzyme L160W, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.03
-
 ubiquinol-1 mutant enzyme Q101A, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.045
-
 ubiquinol-1 mutant enzyme Q82A, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.05
-
 ubiquinol-1 wild type enzyme, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.072
-
 ubiquinol-1 mutant enzyme F93Y, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.072
-
 ubiquinol-1 mutant enzyme Q101N, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.091
-
 ubiquinol-1 mutant enzyme F93A, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.1
-
 ubiquinol-1 mutant enzyme N157V, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.124
-
 ubiquinol-1 mutant enzyme Q101E, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.141
-
 ubiquinol-1 mutant enzyme Q101L, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.2
-
 ubiquinol-1 mutant enzyme Q101T, at pH 7.0 and 25°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.1.3 Cu2+ contains copper Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.1.1.3 ubiquinol-8 + O2 + H+[side 1] Escherichia coli
-
 ubiquinone-8 + H2O + H+[side 2]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.3 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.3 ubiquinol-1 + O2 + H+[side 1]
-
 Escherichia coli ubiquinone-1 + H2O + H+[side 2]
-
 ?
7.1.1.3 ubiquinol-2 + O2 + H+[side 1]
-
 Escherichia coli ubiquinone-2 + H2O + H+[side 2]
-
 ?
7.1.1.3 ubiquinol-8 + O2 + H+[side 1]
-
 Escherichia coli ubiquinone-8 + H2O + H+[side 2]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.1.1.3 cytochrome bo3 ubiquinol oxidase
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.3 heme
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
7.1.1.3 0.000005
-
 aurachin C1-10 wild type enzyme, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.0007
-
 2-n-heptyl-4-hydroxyquinoline N-oxide wild type enzyme, at pH 7.0 and 25°C Escherichia coli