EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.3.192 | expression in Escherichia coli and in an engineered Streptomyces host.Recombinant SCLAV_p0490 forms insoluble proteins | Streptomyces clavuligerus |
4.2.3.193 | expression in Escherichia coli and in an engineered Streptomyces host.Recombinant SCLAV_p0490 forms insoluble proteins | Streptomyces cyslabdanicus |
5.5.1.30 | expression in Escherichia coli | Streptomyces clavuligerus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.5.1.30 | Mg2+ | assay in presence of | Streptomyces clavuligerus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.192 | (+)-copalyl diphosphate | Streptomyces clavuligerus | - |
labda-8(17),13(16),14-triene + diphosphate | - |
? | |
4.2.3.192 | (+)-copalyl diphosphate | Streptomyces clavuligerus DSM 738 | - |
labda-8(17),13(16),14-triene + diphosphate | - |
? | |
4.2.3.192 | labda-7,13-dienyl diphosphate | Streptomyces clavuligerus | - |
labda-7,13(16),14-triene + diphosphate | - |
? | |
4.2.3.192 | labda-7,13-dienyl diphosphate | Streptomyces clavuligerus DSM 738 | - |
labda-7,13(16),14-triene + diphosphate | - |
? | |
4.2.3.193 | (+)-copalyl diphosphate | Streptomyces cyslabdanicus | - |
labda-8(17),12,14-triene + diphosphate | - |
? | |
4.2.3.193 | labda-7,13-dienyl diphosphate | Streptomyces cyslabdanicus | - |
labda-7,12,14-triene + diphosphate | - |
? | |
4.2.3.193 | additional information | Streptomyces cyslabdanicus | the ionization of (+)-copalyl diphosphate or labda-7,13-dienyl diphosphate is initiated by the removal of diphosphate, and the resultant cation is then quenched by the deprotonation at H-12 | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.3.192 | Streptomyces clavuligerus | B5GRC8 | - |
- |
4.2.3.192 | Streptomyces clavuligerus DSM 738 | B5GRC8 | - |
- |
4.2.3.193 | Streptomyces cyslabdanicus | A0A0H5BN57 | - |
- |
5.5.1.30 | Streptomyces clavuligerus | D5SJ87 | - |
- |
5.5.1.30 | Streptomyces clavuligerus ATCC 27064 | D5SJ87 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.192 | (+)-copalyl diphosphate | - |
Streptomyces clavuligerus | labda-8(17),13(16),14-triene + diphosphate | - |
? | |
4.2.3.192 | (+)-copalyl diphosphate | - |
Streptomyces clavuligerus DSM 738 | labda-8(17),13(16),14-triene + diphosphate | - |
? | |
4.2.3.192 | labda-7,13-dienyl diphosphate | - |
Streptomyces clavuligerus | labda-7,13(16),14-triene + diphosphate | - |
? | |
4.2.3.192 | labda-7,13-dienyl diphosphate | - |
Streptomyces clavuligerus DSM 738 | labda-7,13(16),14-triene + diphosphate | - |
? | |
4.2.3.192 | additional information | the ionization of (+)-copalyl diphosphate or labda-7,13-dienyl diphosphate is initiated by the removal of diphosphate, and the resultant cation is then quenched by the deprotonation at H-16 | Streptomyces clavuligerus | ? | - |
? | |
4.2.3.192 | additional information | the ionization of (+)-copalyl diphosphate or labda-7,13-dienyl diphosphate is initiated by the removal of diphosphate, and the resultant cation is then quenched by the deprotonation at H-16 | Streptomyces clavuligerus DSM 738 | ? | - |
? | |
4.2.3.193 | (+)-copalyl diphosphate | - |
Streptomyces cyslabdanicus | labda-8(17),12,14-triene + diphosphate | - |
? | |
4.2.3.193 | labda-7,13-dienyl diphosphate | - |
Streptomyces cyslabdanicus | labda-7,12,14-triene + diphosphate | - |
? | |
4.2.3.193 | additional information | the ionization of (+)-copalyl diphosphate or labda-7,13-dienyl diphosphate is initiated by the removal of diphosphate, and the resultant cation is then quenched by the deprotonation at H-12 | Streptomyces cyslabdanicus | ? | - |
? | |
5.5.1.30 | geranylgeranyl diphosphate | - |
Streptomyces clavuligerus | (13E)-labda-7,13-dien-15-yl diphosphate | - |
? | |
5.5.1.30 | geranylgeranyl diphosphate | - |
Streptomyces clavuligerus ATCC 27064 | (13E)-labda-7,13-dien-15-yl diphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.3.192 | SCLAV_p0491 | - |
Streptomyces clavuligerus |
4.2.3.193 | CldD | - |
Streptomyces cyslabdanicus |
4.2.3.193 | labdatriene synthase | - |
Streptomyces cyslabdanicus |
5.5.1.30 | prenyltransferase and squalene oxidase repeat protein | - |
Streptomyces clavuligerus |
5.5.1.30 | SCLAV_p0490 | - |
Streptomyces clavuligerus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.2.3.192 | physiological function | in an engineered Streptomyces host, labda-8(17),13(16),14-triene is generated by coexpression of SCLAV_p0491 and diterpene synthase CldB | Streptomyces clavuligerus |
4.2.3.193 | physiological function | in an engineered Streptomyces host, labda-8(17),13(16),14-triene is generated by coexpression of SCLAV_p0491 and diterpene synthase CldB | Streptomyces cyslabdanicus |
5.5.1.30 | physiological function | SCLAV_p0490 catalyzes the generation of labda-8(17),13-dienyl diphosphate by the release of a proton at H-7 in the C8-carbonium ion of (+)-copalyl diphosphate | Streptomyces clavuligerus |