EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.83 | additional information | the enzyme is not stimulated by addition of 2-oxoglutarate | Streptomyces roseochromogenus | |
1.13.12.23 | additional information | the enzyme is not stimulated by addition of 2-oxoglutarate | Streptomyces roseochromogenus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.83 | expressed in Escherichia coli BL21(DE3) cells | Streptomyces roseochromogenus |
1.13.12.23 | expressed in Escherichia coli BL21(DE3) cells | Streptomyces roseochromogenus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.83 | Fe2+ | a non-heme iron enzyme, Fe2+ is required for enzyme activity. Replacement of Fe2+ with other monovalent or divalent metal ions (1 mM Cu+, Zn2+, Mg2+, or Mn2+) results in almost complete (95-98%) loss of enzymatic activity | Streptomyces roseochromogenus | |
1.13.12.23 | Fe2+ | a non-heme iron enzyme, Fe2+ is required for enzyme activity. Replacement of Fe2+ with other monovalent or divalent metal ions (1 mM Cu+, Zn2+, Mg2+, or Mn2+) results in almost complete (95-98%) loss of enzymatic activity | Streptomyces roseochromogenus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.83 | 3-dimethylallyl-4-hydroxyphenylpyruvate + O2 | Streptomyces roseochromogenus | - |
3-dimethylallyl-4-hydroxymandelate + CO2 | - |
? | |
1.13.11.83 | 3-dimethylallyl-4-hydroxyphenylpyruvate + O2 | Streptomyces roseochromogenus DS 12976 | - |
3-dimethylallyl-4-hydroxymandelate + CO2 | - |
? | |
1.13.12.23 | 3-dimethylallyl-4-hydroxymandelate + O2 | Streptomyces roseochromogenus | - |
3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O | - |
? | |
1.13.12.23 | 3-dimethylallyl-4-hydroxymandelate + O2 | Streptomyces roseochromogenus DS 12976 | - |
3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.83 | Streptomyces roseochromogenus | Q8GHB1 | - |
- |
1.13.11.83 | Streptomyces roseochromogenus DS 12976 | Q8GHB1 | - |
- |
1.13.12.23 | Streptomyces roseochromogenus | Q8GHB1 | - |
- |
1.13.12.23 | Streptomyces roseochromogenus DS 12976 | Q8GHB1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.83 | glutathione-Sepharose column chromatography | Streptomyces roseochromogenus |
1.13.12.23 | glutathione-Sepharose column chromatography | Streptomyces roseochromogenus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.83 | 3-dimethylallyl-4-hydroxyphenylpyruvate + O2 | - |
Streptomyces roseochromogenus | 3-dimethylallyl-4-hydroxymandelate + CO2 | - |
? | |
1.13.11.83 | 3-dimethylallyl-4-hydroxyphenylpyruvate + O2 | - |
Streptomyces roseochromogenus DS 12976 | 3-dimethylallyl-4-hydroxymandelate + CO2 | - |
? | |
1.13.12.23 | 3-dimethylallyl-4-hydroxymandelate + O2 | - |
Streptomyces roseochromogenus | 3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O | - |
? | |
1.13.12.23 | 3-dimethylallyl-4-hydroxymandelate + O2 | - |
Streptomyces roseochromogenus DS 12976 | 3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.11.83 | ? | x * 30500, calculated from amino acid sequence | Streptomyces roseochromogenus |
1.13.12.23 | ? | x * 30500, calculated from amino acid sequence | Streptomyces roseochromogenus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.83 | CloR | - |
Streptomyces roseochromogenus |
1.13.12.23 | CloR | - |
Streptomyces roseochromogenus |