Literature summary extracted from

Sparacino-Watkins, C.E.; Tejero, J.; Sun, B.; Gauthier, M.C.; Thomas, J.; Ragireddy, V.; Merchant, B.A.; Wang, J.; Azarov, I.; Basu, P.; Gladwin, M.T.
Nitrite reductase and nitric-oxide synthase activity of the mitochondrial molybdopterin enzymes mARC1 and mARC2 (2014), J. Biol. Chem., 289, 10345-10358 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.7.2.1	expression in Escherichia coli and in HEK cell	Homo sapiens

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.7.2.1	C273A	mutation in the putative active site cysteine residue, known to coordinate molybdenum binding. NO formation is abolished by the C273A mutation	Homo sapiens

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.7.2.1	Tungsten	replacement of molybdenum by tungsten abolishes NO formation	Homo sapiens

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.7.2.1	9.5	-	nitrite	pH 7.4, temperature not specified in the publication	Homo sapiens

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.7.2.1	mitochondrion	-	Homo sapiens	5739	-

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.7.2.1	Molybdenum	nitrite reduction is catalyzed by molybdenum in the active site	Homo sapiens

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.7.2.1	Homo sapiens	Q5VT66	-	-
1.7.2.1	Homo sapiens	Q969Z3	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.7.2.1	additional information	mARC can generate nitric oxide from nitrite when forming an electron transfer chain with NADH, cytochrome b5, and NADH-dependent cytochrome b5 reductase	Homo sapiens	?	-	?
1.7.2.1	nitrite + ferrocytochrome b5 + 2 H+	-	Homo sapiens	nitric oxide + H2O + ferricytochrome b5	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.7.2.1	?	x * 35000, SDS-PAGE, recombinant His-tagged protein lacking the mitochondrial translocation motif	Homo sapiens

Synonyms

EC Number	Synonyms	Comment	Organism
1.7.2.1	mARC1	-	Homo sapiens
1.7.2.1	mARC2	-	Homo sapiens
1.7.2.1	mitochondrial amidoxime reducing component	-	Homo sapiens

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.7.2.1	0.1	-	nitrite	pH 7.4, temperature not specified in the publication	Homo sapiens

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.7.2.1	6.5	-	-	Homo sapiens

pH Range

EC Number	pH Minimum	pH Maximum	Comment	Organism
1.7.2.1	7.5	-	3fold decrease in the rate of NO formation compared to pH 6.5	Homo sapiens

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Release 2023.1 (January 2023)