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Literature summary extracted from

  • Qi, X.H.; Zhu, J.F.; Yun, J.H.; Lin, J.; Qi, Y.L.; Guo, Q.; Xu, H.
    Enhanced xylitol production expression of xylitol dehydrogenase from Gluconobacter oxydans and mixed culture of resting cell (2016), J. Biosci. Bioeng., 122, 257-262 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.9 gene xdh, cloning from strain CGMCC 1.49, DNA and amino acid sequence determination and analysis, functional recombinant overexpression of His6-tagged enzyme in Escherichia coli strain BL21 Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.9 xylitol + NAD+ Gluconobacter oxydans
-
 D-xylulose + NADH + H+
-
 ?
1.1.1.9 xylitol + NAD+ Gluconobacter oxydans CGMCC 1.49
-
 D-xylulose + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.9 Gluconobacter oxydans A0A141BGH5
-
-
1.1.1.9 Gluconobacter oxydans CGMCC 1.49 A0A141BGH5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.9 recombinant His6-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration Gluconobacter oxydans

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.9 2.1
-
 purified native enzyme, substrate xylitol, pH 7.0, 30°C Gluconobacter oxydans
1.1.1.9 14.2
-
 purified recombinant His6-tagged enzyme, substrate D-mannitol, pH 11.0, 30°C Gluconobacter oxydans
1.1.1.9 14.2
-
 purified recombinant His6-tagged enzyme, substrate xylitol, pH 7.0, 30°C Gluconobacter oxydans
1.1.1.9 87.8
-
 purified recombinant His6-tagged enzyme, substrate xylitol, pH 11.0, 30°C Gluconobacter oxydans
1.1.1.9 135.2
-
 purified recombinant His6-tagged enzyme, substrate D-sorbitol, pH 11.0, 30°C Gluconobacter oxydans
1.1.1.9 151.4
-
 purified recombinant His6-tagged enzyme, substrate D-fructose, pH 11.0, 30°C Gluconobacter oxydans
1.1.1.9 326.3
-
 purified recombinant His6-tagged enzyme, substrate D-xylulose, pH 11.0, 30°C Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.9 D-mannitol + NAD+ low activity compared to xylitol Gluconobacter oxydans D-fructose + NADH + H+
-
 r
1.1.1.9 D-mannitol + NAD+ low activity compared to xylitol Gluconobacter oxydans CGMCC 1.49 D-fructose + NADH + H+
-
 r
1.1.1.9 D-sorbitol + NAD+ 51.3% increased activity compared to xylitol Gluconobacter oxydans L-sorbose + NADH + H+
-
 ?
1.1.1.9 D-sorbitol + NAD+ 51.3% increased activity compared to xylitol Gluconobacter oxydans CGMCC 1.49 L-sorbose + NADH + H+
-
 ?
1.1.1.9 xylitol + NAD+
-
 Gluconobacter oxydans D-xylulose + NADH + H+
-
 ?
1.1.1.9 xylitol + NAD+
-
 Gluconobacter oxydans D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+
-
 Gluconobacter oxydans CGMCC 1.49 D-xylulose + NADH + H+
-
 ?
1.1.1.9 xylitol + NAD+
-
 Gluconobacter oxydans CGMCC 1.49 D-xylulose + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.9 ? x * 28000, yes recombinant His6-tagged enzyme, SDS-PAGE Gluconobacter oxydans

Synonyms

EC Number Synonyms Comment Organism
1.1.1.9 XDH
-
 Gluconobacter oxydans
1.1.1.9 xylitol dehydrogenase
-
 Gluconobacter oxydans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.9 30
-
 oxidation of xylitol Gluconobacter oxydans
1.1.1.9 35
-
 reduction of D-xylulose Gluconobacter oxydans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.9 25 40 over 80% of maximal activity within this range for both reaction directions Gluconobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.9 5
-
 reduction of D-xylulose Gluconobacter oxydans
1.1.1.9 11
-
 oxidation of xylitol Gluconobacter oxydans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.9 4 8 over 60% of maximal activity within this range for oxidation of xylitol, profile overview Gluconobacter oxydans
1.1.1.9 10 12 over 60% of maximal activity within this range for reduction of D-xylulose, profile overview Gluconobacter oxydans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.9 NAD+
-
 Gluconobacter oxydans

General Information

EC Number General Information Comment Organism
1.1.1.9 evolution XDH has a TGXXGXXG NAD(H)-binding motif and a YXXXK active site motif, and belongs to the short-chain dehydrogenase/ reductase family Gluconobacter oxydans