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Literature summary extracted from

  • Chen, X.; Berim, A.; Dayan, F.E.; Gang, D.R.
    A (-)-kolavenyl diphosphate synthase catalyzes the first step of salvinorin A biosynthesis in Salvia divinorum (2017), J. Exp. Bot., 68, 1109-1122 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
5.5.1.28 A314V mutation does not change the product outcome Salvia divinorum
5.5.1.28 A314V/F255H mutation leads to the recovery of the ability to produce (-)-kolavenyl diphosphate of wild-type while retaining the acquired function of forming ent-copalyl diphosphate of mutant F255H Salvia divinorum
5.5.1.28 F255H mutation in direction to copalyl diphosphate synthase, mutant shows a complete switch of product specificity from (-)-kolavenyl diphosphate to ent-copalyl diphosphate Salvia divinorum
5.5.1.28 V200I mutation does not change the product outcome Salvia divinorum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.5.1.28 0.0019
-
 geranylgeranyl diphosphate pH 7.0, 30°C Salvia divinorum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.5.1.28 geranylgeranyl diphosphate Salvia divinorum
-
 (-)-kolavenyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.28 Salvia divinorum A0A1S5RW73
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.5.1.28 leaf peltate glandular trichome at abaxial surface and vein Salvia divinorum
-
5.5.1.28 stem peltate glandular trichome at abaxial surface Salvia divinorum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.28 geranylgeranyl diphosphate
-
 Salvia divinorum (-)-kolavenyl diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.5.1.28 KPS
-
 Salvia divinorum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.5.1.28 0.88
-
 geranylgeranyl diphosphate pH 7.0, 30°C Salvia divinorum

General Information

EC Number General Information Comment Organism
5.5.1.28 physiological function enzyme catalyzes the first step of salvinorin A biosynthesis in Salvia divinorum. Transient expression in leaves of Nicotiana benthamiana leads to accumulation of (-)-kolavenol Salvia divinorum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.5.1.28 470
-
 geranylgeranyl diphosphate pH 7.0, 30°C Salvia divinorum