EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.9 | NADH | product inhibition | Aspergillus carbonarius |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.9 | xylitol + NAD+ | Aspergillus carbonarius | - |
D-xylulose + NADH + H+ | - |
? | |
1.1.1.9 | xylitol + NAD+ | Aspergillus carbonarius ITEM 5010 | - |
D-xylulose + NADH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.9 | Aspergillus carbonarius | - |
- |
- |
1.1.1.9 | Aspergillus carbonarius ITEM 5010 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.9 | xylitol + NAD+ | - |
Aspergillus carbonarius | D-xylulose + NADH + H+ | - |
? | |
1.1.1.9 | xylitol + NAD+ | - |
Aspergillus carbonarius ITEM 5010 | D-xylulose + NADH + H+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.9 | XDH | - |
Aspergillus carbonarius |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.9 | NAD+ | - |
Aspergillus carbonarius |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.9 | metabolism | in order to overcome xylitol accumulation in Aspergillus carbonarius, a K274R point mutation is introduced into the xylose reductase with the aim of changing the specificity toward NADH. Fermentation with the mutant strain (grown on D-xylose as the sole carbon source) shows a 2.8fold reduction in xylitol accumulation and 4.5fold increase in citric acid production compared to the wild-type strain. The fact that the mutant strain shows decreased xylitol levels is assumed to be associated with the capability of the mutated xylose reductase to use NADH generated by the xylitol dehydrogenase XDH, thus preventing the inhibition of XDH by the high levels of NADH and ensuring the flux of D-xylose through the pathway | Aspergillus carbonarius |